- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT01956084
Cytotoxic T Cells to Treat Relapsed EBV-positive Lymphoma (ALCI2)
ADMINISTRATION OF LMP-SPECIFIC CYTOTOXIC T-LYMPHOCYTES TO PATIENTS WITH RELAPSED EBV-POSITIVE LYMPHOMA
In this study, investigators are trying to see if LMP specific cytotoxic T lymphocytes (CTLs) will prevent or treat disease called Epstein Barr Virus (EBV) Disorder including either Hodgkin Lymphoma or non-Hodgkin Lymphoma or Lymphoepithelioma or severe chronic active EBV infection syndrome (SCAEBV) or Leiomyosarcoma which has come back or has not gone away after treatment, including the best treatment. Investigators are using special immune system cells called third party LMP specific cytotoxic T lymphocytes (CTLs), a new experimental therapy.
Some patients with Lymphoma or SCAEBV or Leiomyosarcoma show evidence of infection with the virus that causes infectious mononucleosis Epstein Barr virus (EBV) before or at the time of their diagnosis. EBV is found in the cancer cells of up to half the patients with Hodgkin's and non-Hodgkin Lymphoma, suggesting that it may play a role in causing Lymphoma. The cancer cells (in lymphoma) and some B cells (in SCAEBV) infected by EBV are able to hide from the body's immune system and escape destruction. The investigators want to see if special white blood cells, called T cells, that have been trained to kill EBV infected cells can survive in patient's blood and affect the tumor or infection.
Investigators used this sort of therapy to treat a different type of cancer that occurs after bone marrow or solid organ transplant called post transplant lymphoma. In this type of cancer the tumor cells have 9 proteins made by EBV on their surface. They grew T cells in the laboratory that recognized all 9 proteins and were able to successfully prevent and treat post transplant lymphoma. However in Hodgkin Lymphoma, the tumor cells and B cells only express 2 EBV proteins. In a previous study they made T cells that recognized all 9 proteins and gave them to patients with Hodgkin Lymphoma. Some patients had a partial response to this therapy but no patients had a complete response. They think one reason may be that many of the T cells reacted with proteins that were not on the tumor cells. In this present study the investigators are trying to find out if the investigators can improve this treatment by growing T cells that recognize proteins expressed on EBV infected Lymphoma cells and B cells called LMP-1 and LMP2. These special T cells are called third party LMP 1/2 -specific cytotoxic T-lymphocytes (CTLs). These LMP-specific cytotoxic
T cells are an investigational product not approved by the Food and Drug Administration.
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
Investigators will first test a biopsy of the tumor or lymph node that has already been done to see if the tumor or tissue cells are EBV positive. If the patient is eligible, investigators will then take 60 mL (about 12 teaspoons) of blood from the patient or their donor on one or two occasions. They will use this blood to grow T cells. First they will grow a special type of cells called dendritic cells or monocytes which will stimulate the T cells. Next they will put a specially produced human virus that carries the LMP genes into the dendritic cells or monocytes. They will then be used to stimulate T cells. This stimulation will train the T cells to kill cells with LMP on their surface. Investigators will then grow these LMP specific CTLs by more stimulation with EBV infected cells. These EBV infected cells will be treated with radiation so they cannot grow.
Once sufficient numbers of T cells have been made, investigators will test them to make sure they kill cells with LMP on their surface. If the counts are low they may need to obtain additional blood samples to make these cells. Prior to giving the patient the CTLs, the cells will be tested to make sure they don't attack the tissue.
The cells will then be thawed and injected into the patient over 10 minutes. Initially, two doses of T cells will be given two weeks apart. If after the second infusion there is a reduction in the size of the lymphoma on CT or MRI scan as assessed by a radiologist, the patient can receive up to six additional doses of the T cells if the patient wishes. This is a dose escalation study which means that for some patients the second dose may be larger than the first. All of the treatments will be given by the Center for Cell and Gene Therapy at Texas Children's Hospital or the Methodist Hospital.
For follow-up after the CTL infusions, the patient will be seen every 3 months for the first year. Then the patient will either be seen in the clinic or they will be contacted by a research nurse yearly for 5 years.
Study Type
Enrollment (Estimated)
Phase
- Phase 1
Contacts and Locations
Study Locations
-
-
District of Columbia
-
Washington, District of Columbia, United States, 20010
- Childrens National Medical Center
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
- Child
- Adult
- Older Adult
Accepts Healthy Volunteers
Description
Inclusion Criteria:
- Any patient, regardless of age or sex, with a diagnosis of EBV positive Hodgkin's or non-Hodgkin's Lymphoma or EBV (associated)-T/NK-LPD lymphoproliferative disease or Lymphoepithelioma/leiomyosarcoma regardless of histological subtype or Severe Chronic EBV and in remission (group A) or with detectable disease (group B) after allogeneic SCT
- Patients with life expectancy > 6 weeks.
- Tumor tissue EBV positive
- Patients with a Karnofsky/Lansky score of > 50
- Donor HIV negative
- must not have less than 50% donor chimerism in either peripheral blood or bone marrow
- Patients with bilirubin <2x normal, AST <5x normal, and Hgb >8.0
- Patients with a creatinine <2x normal for age
- Patients should have been off other investigational therapy for one month prior to entry in this study.
- Patient, parent/guardian able to give informed consent.
Exclusion Criteria:
- Donors who are HIV positive
- Patients with GVHD > Grade II
- Due to unknown effects of this therapy on a fetus, pregnant women are excluded from this research.
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Treatment
- Allocation: Non-Randomized
- Interventional Model: Single Group Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: LMP1/2 CTLs (Group A)
Patients receiving CTLs as adjunctive therapy following allogeneic stem transplant
|
Each patient will receive 2 injections, 14 days apart, according to the following dosing schedules: Dose Level One: Day 0: 1 x 107 cells/m2 Day 14: 1 x 107 cells/m2 Dose Level Two: Day 0: 2 x 107 cells/m2 Day 14: 2 x 107 cells/m2 Dose Level Three: Day 0: 5 x 107 cells/m2 Day 14: 5 x 107 cells/m2 |
Experimental: LMP1/2 CTLs (Group B)
Patients receiving CTLs in relapse following allogeneic stem cell transplant
|
Each patient will receive 2 injections, 14 days apart, according to the following dosing schedules: Dose Level One: Day 0: 1 x 107 cells/m2 Day 14: 1 x 107 cells/m2 Dose Level Two: Day 0: 2 x 107 cells/m2 Day 14: 2 x 107 cells/m2 Dose Level Three: Day 0: 5 x 107 cells/m2 Day 14: 5 x 107 cells/m2 |
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Number of patients with dose limiting toxicity (DLT)
Time Frame: 6 weeks
|
Toxicity will be evaluated according to NCI Common Terminology Criteria for Adverse Events scale, version 2.0.
DLT will be defined as development of any toxicity scored as Grade 3 or 4 and primarily related to the CTL infusion.
|
6 weeks
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Survival and Immune Function of LMP-specific CTLs
Time Frame: 5 years
|
Survival and function will be measured by frequencies and immunological parameters from (enzyme-linked immunospot) ELISPOT and cytotoxicity assays.
|
5 years
|
Other Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
To obtain preliminary information on the safety and response to an extended dosage regimen.
Time Frame: 6 weeks
|
Comparison of diagnostic imaging studies from pre-infusion to 6 weeks after the second infusion will be summarized.
Frequencies and proportions of responders will be summarized overall and by dose levels if there are enough patients per dose level.
|
6 weeks
|
Collaborators and Investigators
Sponsor
Investigators
- Principal Investigator: Catherine Bollard, MD, CNMC
Publications and helpful links
Study record dates
Study Major Dates
Study Start
Primary Completion (Estimated)
Study Completion (Estimated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Estimated)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
- Virus Diseases
- Infections
- Immune System Diseases
- Neoplasms, Connective and Soft Tissue
- Neoplasms by Histologic Type
- Neoplasms
- Lymphoproliferative Disorders
- Lymphatic Diseases
- Immunoproliferative Disorders
- DNA Virus Infections
- Tumor Virus Infections
- Herpesviridae Infections
- Sarcoma
- Neoplasms, Muscle Tissue
- Lymphoma
- Hodgkin Disease
- Leiomyosarcoma
- Epstein-Barr Virus Infections
Other Study ID Numbers
- ALCI2
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
Clinical Trials on Hodgkin Disease
-
Tessa TherapeuticsActive, not recruitingHodgkin Lymphoma, Adult | Hodgkin Disease Recurrent | Hodgkin Disease Refractory | Hodgkin Disease, PediatricUnited States
-
Tessa TherapeuticsBristol-Myers SquibbActive, not recruitingClassical Hodgkin Lymphoma | Hodgkin Disease Recurrent | Hodgkin Disease RefractoryUnited States
-
Shandong Provincial HospitalUnknownLymphoma, Non-Hodgkin;Hodgkin DiseaseChina
-
AkesoPeking University Cancer Hospital & InstituteRecruiting
-
Stanford UniversityCompletedLymphoma | Hodgkin Disease | Lymphoma, Hodgkin Disease | Lymphoma: HodgkinUnited States
-
Grupo Argentino de Tratamiento de la Leucemia AgudaTakedaRecruitingRefractory Hodgkin Lymphoma | Hodgkin Lymphoma, Adult | Relapsed Hodgkin's Disease, AdultArgentina
-
University of WashingtonRecruitingRecurrent Hodgkin Lymphoma | Refractory Hodgkin Lymphoma | Recurrent Non-Hodgkin Lymphoma | Refractory Non-Hodgkin LymphomaUnited States
-
Seagen Inc.Millennium Pharmaceuticals, Inc.CompletedDisease, HodgkinUnited States, United Kingdom, France, Romania, Poland, Italy, Bulgaria, Hungary, Russian Federation, Czechia, Spain, Germany, Serbia
-
Seagen Inc.Millennium Pharmaceuticals, Inc.CompletedDisease, HodgkinUnited States, France, Canada, Belgium, Italy
-
Seagen Inc.Millennium Pharmaceuticals, Inc.CompletedDisease, HodgkinUnited States, Canada
Clinical Trials on LMP1/2 CTLs (Group A)
-
Baylor College of MedicineThe Methodist Hospital Research Institute; Center for Cell and Gene Therapy...CompletedHodgkin Disease | Non Hodgkin Lymphoma | Leiomyosarcoma | LymphoepitheliomaUnited States
-
Beijing Biostar Pharmaceuticals Co., Ltd.RecruitingAdvanced Solid TumorChina
-
Cairo UniversityCompleted
-
Bukwang PharmaceuticalActive, not recruitingParkinson Disease | DyskinesiasUnited States
-
First Affiliated Hospital of Harbin Medical UniversityCompletedDiabetic Angiopathies
-
ThinkWellUniversity of Oxford; Queen's University, Belfast; The BMJCompleted
-
Mahidol UniversityUnknown
-
Actimed Therapeutics LtdQuotient SciencesCompleted
-
ResMedCompleted
-
M.D. Anderson Cancer CenterActive, not recruitingHematopoietic and Lymphoid Cell Neoplasm | Malignant Solid Neoplasm | Symptomatic COVID-19 Infection Laboratory-ConfirmedUnited States