Serum Amyloid A Protein And Fetuin A Levels

Serum Amyloid A Protein And Fetuin A Protein Levels In Periodontal Health And Disease

The purpose of this study was to evaluate acute phase proteins (APPs) Fetuin-A and Serum Amyloid A (SAA) levels in gingival crevicular fluid (GCF) and serum samples in periodontal health and disease.

Study Overview

• Status: Completed
• Conditions: Periodontal Diseases
• Intervention / Treatment: Biological: Fetuin-A; Biological: Serum Amyloid A

Detailed Description

The study population consisted totally 60 subjects consulted to Bulent Ecevit University, Faculty of Dentistry, Department of Periodontology whose age and sex were matched.

According to study design, subjects were divided into three groups based on their periodontal parameters. Patients suffering from chronic periodontitis formed the group 1 (n: 20), patients with plaque induced gingivitis formed the group 2 (n:20) and periodontal healthy patients were inserted in group 3 (n:20).

After gently drying the area, GCF was sampled with filter paper using the intracrevicular method (Periopaper, ProFlow, Inc., Amityville, New York, USA).Two milliliters (ml) of peripheral blood was collected from each individual with sterile syringe from the antecubital fossa by venipuncture, and to separate serum component sample centrifuged at 3000g for 5 min, and immediately stored at -40°C until assayed.

The quantity of Fetuin-A and SAA protein concentration in the samples were analyzed by sandwich enzyme-linked immunosorbent assay (ELISA) procedures using commercially available kits.

The Spearman's rank correlation test was used to detect the relationship between GCF and serum SAA and Fetuin-A with GI and CAL. All tests were performed using statistical software (SPSS Inc., version 22.0, Chicago, IL, USA). The mean ± standard deviation with mean rank values were calculated for each parameter, based on the patients as the statistical unit. P < 0.05 was considered to be statistically significant.

• Study Type: Observational
• Enrollment (Actual): 60

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 28 years to 45 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

The study population consisted totally 60 subjects whose age (28-45) and sex (33 men, 27 female) were matched.

Description

Group 1 Inclusion Criteria:

• GI>1, PPD≥5mm, CAL≥5mm with alveolar bone loss radiographically.
• Systematically healthy patients

Group 2 Inclusion Criteria:

• No alveolar bone and attachment loss
• Inflammation signs such as redness, edema and increased BOP levels,
• GI≥2, PPD≤3mm, CAL≤3mm.
• Systematically healthy patients

Group 3 Inclusion Criteria:

• No bone and attachment loss,
• GI=0, PPD≤3mm, CAL≤3mm
• Systematically healthy patients

Exclusion Criteria for all groups:

• Aggressive Periodontitis,
• Oral pathologies,
• Patients with any other systemic diseases,
• Pregnant women and those in the lactation period,
• Patients with smoking habit and taking medication
• Patients received periodontal therapy in last 6 months

Study Plan

How is the study designed?

Design Details

• Observational Models: Case-Control
• Time Perspectives: Cross-Sectional

Number of groups / cohorts

3

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Group 1; Chronic Periodontitis-serum amyloid A, Fetuin-A	Biological: Fetuin-A; Acute phase protein; Biological: Serum Amyloid A; Acute phase protein
Group 2; Plaque induced Gingivitis-serum amyloid A, Fetuin-A	Biological: Fetuin-A; Acute phase protein; Biological: Serum Amyloid A; Acute phase protein
Group 3; Control Group-serum amyloid A, Fetuin-A	Biological: Fetuin-A; Acute phase protein; Biological: Serum Amyloid A; Acute phase protein

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Serum Amyloid A (SAA) levels as an inflammatory marker in periodontal disease both in serum and GCF	Day 0

Secondary Outcome Measures

Outcome Measure	Time Frame
Fetuin-A levels as an inflammatory marker in periodontal disease both in serum and GCF	Day 0

Collaborators and Investigators

• Sponsor: Bulent Ecevit University
• Investigators: Principal Investigator: ÇİĞDEM COŞKUN TÜRER, PhD, BULENT ECEVIT UNIVERSITY FACULTY OF DENTISTRY DEPARTMENT OF PERIODONTOLOGY

Study record dates

Study Major Dates

• Study Start: December 1, 2013
• Primary Completion (Actual): April 1, 2014
• Study Completion (Actual): November 1, 2014

Study Registration Dates

• First Submitted: April 24, 2015
• First Submitted That Met QC Criteria: April 29, 2015
• First Posted (Estimate): May 5, 2015

Study Record Updates

• Last Update Posted (Estimate): May 5, 2015
• Last Update Submitted That Met QC Criteria: April 29, 2015
• Last Verified: April 1, 2015

More Information

Terms related to this study

• Keywords: serum; periodontal diseases; gingival crevicular fluid; Serum Amyloid A; Fetuin-A
• Additional Relevant MeSH Terms: Stomatognathic Diseases; Mouth Diseases; Periodontal Diseases
• Other Study ID Numbers: SAAFA1