Circulatory Levels of Irisin in Response to Chronic Resistance Training

Study the responses of circulatory irisin to chronic high-intensity resistance training

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Other: Exercise Training

Detailed Description

Objective: The purpose of this study is to study the circulating levels of irisin in response to chronic high-intensity resistance training in a group of healthy young adults.

Sample: The sample will be comprised 44 of adult men and women unpaid volunteers between the ages of 18 and 30.

Pre-screening assessments:

Informed consent, PAR-Q, Medical History and Exercise History will be used to determine health status and prior exercise experience. Menstrual cycle log will be required for female participants.

Pre-Intervention assessments:

• Anthropometric measurements and Body Composition: Height, triceps, thigh and calf skinfolds, biceps, thigh, hip, waist, and calf circumferences will be measured. Dual-energy x-ray absorptiometry (DEXA) will be performed to obtain body composition.
• Cardiopulmonary and Strength Fitness Testing. Cardiopulmonary fitness will be determined via maximum oxygen uptake testing. One maximum repetition (1-RM) will be used to assess the muscular fitness.
• Spontaneous Physical Activity: Daily activity level for 7 days will be collected.
• Familiarization. Participant will be instructed on the muscle conditioning techniques used for strength training so that the techniques can be mastered. The rate of perceived exertion (RPE) scale will also be used for familiarization during these sessions.

Intervention: The intervention will last 3 weeks with a frequency of 3 training session per week. We will collect blood samples levels at session 1 (at the beginning of week 1), session 3 (at the end of week 1), at session 6 (at the end of week 2) and session 9 (at the end of week 3). Blood draws will occur at the beginning of each session evaluated (pre-session), at the 45 min mark during each session and immediately after each session (post-session).

The sessions will consist on a circuit training of 3 sets of 7 exercises focused on major muscle groups. Ten repetitions on each circuit at 70% of the maximum capacity will be performed.

During the intervention:

- Blood-related variables. Baseline glucose, lactate and hematocrit will be obtained from all participants prior to intervention. Lactate and Hematocrit will be obtained pre-intervention and post-intervention, and within 1 min post workout at sessions 1, 3, 6 and 9.

Irisin will be obtained from blood samples at baseline, during and after training sessions 1, 3, 6 and 9. Irisin will be measured via a commercial enzyme-linked immunosorbent assay.

- Dietary Controls. Each participant will complete the Food Preference Questionnaire. In addition, the Automated Self-Administered 24-hour Recall (ASA24) will be collected for the days prior to sessions 1, 3, 6 and 9.

Post-Intervention assessments:

• Anthropometric measurements and Body Composition: Height, triceps, thigh and calf skinfolds, biceps, thigh, hip, waist, and calf circumferences will be measured. Dual-energy x-ray absorptiometry (DEXA) will be performed to obtain body composition.
• Caridorespiratory capacity determined via maximum oxygen uptake testing. One maximum repetition (1-RM) will be used to assess the muscular fitness.
• Spontaneous Physical Activity: Daily activity level for 7 days will be collected.

• Study Type: Interventional
• Enrollment (Actual): 28
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Texas
    • Lubbock, Texas, United States, 79423
      • Exercise and Sport Sciences Building

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Exclusion Criteria:

• Known health problems such as cardiovascular, pulmonary or metabolic disease will be excluded.
• Pregnant females.
• Any adults found taking medications that affect endocrine or cardiovascular function will also be excluded from the study.
• Individuals found to be less than 110 pounds.
• Participants that exercise more than three times per week or strength train more than twice per week.
• Individuals that have poor venous access or have expressed a fear of needles or having blood taken will also be excluded.
• Cigarette smoking (current or those who quit within the previous 6 months
• Hypertension
• Overweight and obese
• Pre-diabetics

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
No Intervention: Control
Experimental: Intervention; The acute intervention will consist of 1 session of training following the program: Resistance Training Exercises. Each participant in the intervention group will perform resistance-training exercises in the form of a circuit. The circuit will consist of 10 repetitions per exercise of 7 exercises: leg press, bent-over row, bench press, squats, dumbbell jump squats with raises, dead-lifts and weighted abdominal crunches, with approximately 30 sec of rest in between each exercise (based on the estimated time needed to move from one position to the next). Initial intensity 6-7 of RPE and ending the set at 9-10. Each participant will move through the circuit 3 times, with 2 to 3 minutes of rest between each round.	Other: Exercise Training; Chronic High-Intensity Resistance Training

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in Serum irisin levels		Baseline and 3 weeks
Blood Lactate levels	Intensity of exercise on sessions 1, 3, 6 and 9	3 weeks
Changes in physical fitness levels as meassured by 1RM (repetition maximum) and Cardio-respiratory maximal testing		Baseline and 3 weeks
Changes in Body composition assessment: DEXA and anthropometry		Baseline and 3 weeks
Changes in Blood glucose levels		Baseline and 3 weeks
Changes in Hematocrit concentration		Baseline and 3 weeks
Diet assessment: 24 hour recall and Food Preference Questionnaire		3 weeks
Physical Activity assessment: accelerometry		3 weeks

Collaborators and Investigators

• Sponsor: Texas Tech University
• Investigators: Principal Investigator: Maria Fernandez del Valle, PhD, Texas Tech University

Study record dates

Study Major Dates

• Study Start: February 1, 2015
• Primary Completion (Actual): October 1, 2015

Study Registration Dates

• First Submitted: February 3, 2015
• First Submitted That Met QC Criteria: June 17, 2015
• First Posted (Estimate): June 18, 2015

Study Record Updates

• Last Update Posted (Estimate): October 20, 2015
• Last Update Submitted That Met QC Criteria: October 19, 2015
• Last Verified: September 1, 2015

More Information

Terms related to this study

• Keywords: Volunteers
• Other Study ID Numbers: 504792B