Bifidobacteria In Children With Abdominal Pain-Associated Functional Gastrointestinal Disorders

October 1, 2015 updated by: Erasmo Miele, Federico II University

A Multicentre, Randomised, Double-Blind, Placebo Controlled, Crossover Trial On The Efficacy Of A Mixture Of Three Bifidobacteria In Children With Abdominal Pain-Associated Functional Gastrointestinal Disorders

Abdominal pain (AP)-associated functional gastrointestinal disorders (FGIDs), particularly Irritable Bowel Syndrome (IBS) and Functional Dyspepsia (FD), are common in pediatrics, and no safe and effective treatment is available. Although probiotics have shown promising results in adults, few studies have been published in children. The Bifidobacterium Infantis, Bifidobacterium Breve and Bifidobacterium Longum are the most important beneficial bacteria in children and represent 95% of the total bacterial population in the intestine of breastfed infant.

Objectives: 1) To evaluate the effect of oral administration of a mixture of Bifidobacteria on the improvement of frequency and intensity of AP in children with FD and IBS. 2) To evaluate the effect of oral administration of a mixture of Bifidobacteria on quality of life in children with FD and IBS.

Study Overview

Status

Completed

Detailed Description

The study was a randomized, double blind, placebo-controlled, crossover trial conducted at two pediatric tertiary care centers in Naples and Foggia. All children aged 8-17 years referred for IBS or FD to the Pediatric Clinics of the two participating centers between January and December 2014 were eligible for the study. IBS and FD were diagnosed using the Rome III criteria for pediatric FGIDs. The main exclusion criterion was the presence of chronic organic gastrointestinal diseases, assessed by full clinical history and examination, and initial laboratory investigation including complete blood cell count, erythrocyte sedimentation rate, C-reactive protein, serum amylase and lipase, tissue transglutaminase antibodies, total serum immunoglubulins A, and fecal calprotectin. Abnormalities in any of these tests resulted in the patient's exclusion from the study. Further exclusion criteria were previous abdominal surgery, diseases affecting bowel motility, or concomitant psychiatric, neurological, metabolic, renal, hepatic, infectious, hematological, cardiovascular or pulmonary disorders. Finally, patients who had been using any commercial preparation of probiotics during the previous 3 months were also excluded.

The study was articulated in 16 weeks. After recruitment, patients entered a 2 week-run-in phase during which evacuative frequency, stool features and gastrointestinal symptoms were recorded on a daily basis using a questionnaire/diary provided at the study entry by the physician. At the end of the baseline period, patients returned to the center where information regarding AP characteristics, bowel habits and associated symptoms were recorded using a previously validated interviewer-administered questionnaire for pediatric FGIDs. The "Functional Disability Inventory" (FDI), a second interviewer-administered validated questionnaire was used to assess physical and psychosocial functions and investigate patients' QoL. The instrument consists of 15 items concerning perceptions of activity limitations during the past 2 weeks; total scores are computed by summing the ratings for each item. Total available score ranges from 0 to 60 and higher scores indicate greater disability. After completing these questionnaires, patients were assigned in a double-blinded fashion to the placebo or intervention group according to a computer-generated randomization allocation table. Participants were randomized to receive either 1 sachet per day of a mixture of three Bifidobacteria (namely, 3 billions of Bifidobacterium longum BB536®, 1 billion of Bifidobacterium infantis M-63®, and 1 billion of Bifidobacterium breve M-16V®), or an identical looking and tasting placebo for six weeks. No further medication other than analgesics was allowed for the whole duration of the study.

After completing the six weeks of treatment, no preparation was administered for a 2-week-''washout'' period. Afterwards, each patient was switched to the other group and treated with placebo or probiotics for a period of six further weeks.

At each follow-up visit subjects underwent a complete physical examination, data recorded on the daily diaries were collected and compliance to treatment was verified. Furthermore, the FGIDs symptoms questionnaire and the FDI were administered by the physician and answers were recorded.

The main outcome parameters considered for the assessment of the efficacy of the administered treatment were abdominal pain and QoL. The investigators considered a decrease in FDI score of at least 75% of the baseline score to define a relevant improvement in QoL. Secondary outcome parameters were changes in bowel habit for IBS patients, and the effect of the tested treatment on nausea for FD subjects.

The investigators involved in the recruitment and follow-up of patients, those coordinating the study and analyzing the data, patients themselves and their caregivers were all unaware of the randomization group at each phase of the study.

The institutional ethical review boards of both participating centers approved the study protocol. Written informed consent was obtained from the parents or legal guardians before enrollment.

Study Type

Interventional

Enrollment (Actual)

73

Phase

  • Phase 4

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

      • Naples, Italy, 80131
        • University of Naples "Federico II" Italy

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

4 years to 18 years (ADULT, CHILD)

Accepts Healthy Volunteers

No

Genders Eligible for Study

All

Description

Inclusion Criteria:

  • Age 4-18years;
  • Diagnosis of FD and IBS pain according to the Rome III criteria
  • All parents or legal tutors must sign an informed consent document indicating their awareness of the investigational nature of this study.

Exclusion Criteria:

  • Taking any other type of probiotic in the 2 months prior to enrollment
  • Presence of intestinal motility disorders
  • Presence of any other significant medical condition
  • Presence of previous abdominal surgery
  • Inability or unwillingness to give informed consent

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: TREATMENT
  • Allocation: RANDOMIZED
  • Interventional Model: CROSSOVER
  • Masking: DOUBLE

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Active Comparator: Mixture of three Bifidobacteria
Patients were administered 1 sachet per day of a mixture of three Bifidobacteria (namely, 3 billions of Bifidobacterium longum BB536®, 1 billion of Bifidobacterium infantis M-63®, and 1 billion of Bifidobacterium breve M-16V®) for six weeks. Subsequently, no preparation was administered for a 2-week-''washout'' period. At each follow-up visit subjects underwent a complete physical examination, data recorded on the daily diaries were collected and compliance to treatment was verified.
This investigation will be a randomized, double-blind, placebo-controlled, cross-over trial. The study will include 70 children with FD or IBS and will be articulated in 16 weeks as follows. Fifty-nine children (median age 11.2 years, range 5.2-17.9) with IBS and FD were randomized to receive either a mixture of three Bifidobacteria or a placebo for 6 weeks. At the end, after a 2-week-''washout'' period, each patient was switched to the other group and followed for 6 further weeks. At baseline and at follow-up, patients and/or their parents completed a dairy for bowel habit and gastrointestinal symptoms, and a quality of life (QoL) questionnaire.
Other Names:
  • Bifidobacterium infantis M-63® breve M-16V® longum BB536®
Placebo Comparator: Placebo
Patients were administered 1 sachet per day of placebo for six weeks. Subsequently, no preparation was administered for a 2-week-''washout'' period. At each follow-up visit subjects underwent a complete physical examination, data recorded on the daily diaries were collected and compliance to treatment was verified.
This investigation will be a randomized, double-blind, placebo-controlled, cross-over trial. The study will include 70 children with FD or IBS and will be articulated in 16 weeks as follows. Fifty-nine children (median age 11.2 years, range 5.2-17.9) with IBS and FD were randomized to receive either a mixture of three Bifidobacteria or a placebo for 6 weeks. At the end, after a 2-week-''washout'' period, each patient was switched to the other group and followed for 6 further weeks. At baseline and at follow-up, patients and/or their parents completed a dairy for bowel habit and gastrointestinal symptoms, and a quality of life (QoL) questionnaire.
Other Names:
  • Bifidobacterium infantis M-63® breve M-16V® longum BB536®

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Abdominal Pain Frequency
Time Frame: 16 weeks from the enrollment
Score ranges from 0 (never) to 5 (every day)
16 weeks from the enrollment

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Functional Disability
Time Frame: 16 weeks from the enrollment
Functional Disability Inventory questionnaire consisting of 15 items concerning perceptions of activity limitations during the previous 2 weeks. Score for each item ranges from 0 (no trouble) to 4 (impossible), for a total score ranging from 0 (no quality of life impairment) to 60 (maximum impairment in quality of life).
16 weeks from the enrollment

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Investigators

  • Principal Investigator: Eleonora Giannetti, MD, Department of Translational Medical Sciences, Federico II University

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start

December 1, 2013

Primary Completion (Actual)

July 1, 2015

Study Completion (Actual)

September 1, 2015

Study Registration Dates

First Submitted

February 25, 2014

First Submitted That Met QC Criteria

October 1, 2015

First Posted (Estimate)

October 2, 2015

Study Record Updates

Last Update Posted (Estimate)

October 2, 2015

Last Update Submitted That Met QC Criteria

October 1, 2015

Last Verified

September 1, 2015

More Information

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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