Exercise Induced Changes in Fat Metabolism (BC)

Exercise-mediated Changes in Adipocyte Differentiation and Metabolic Function

If eligible to participate in the research study after completing the physical screening procedures, participants will be asked to return to the Substrate Metabolism Laboratory on another occasion after a short fast (no food or beverages - besides water) for at least 6 hours. After participants arrive, participants will rest quietly for approximately 30 min. The investigators will then collect a blood sample from a vein in participants hand, or forearm. After obtaining a baseline blood sample, participants will exercise at a moderate intensity (on a treadmill or a stationary bike) for one hour. This one hour moderate exercise session will feel like a slow jog during which participants would be able to carry a conversation. In order for the investigators to measure energy expended and the amount of fat participants are burning during exercise, the investigators will periodically ask participants to breathe through a mouthpiece so the investigators can collect participants expired breath. After the exercise session the investigators will collect another blood sample from a vein in participants hand, or forearm.

Study Overview

• Status: Completed
• Conditions: Healthy Volunteers
• Intervention / Treatment: Other: Exercise session

Detailed Description

General study design

• Subjects who exercise regularly will be instructed to exercise exactly 3 days before the experimental trial, and then abstain from their exercise training program until after the experiment is complete.
• Subjects will arrive to the Substrate Metabolism Laboratory (1210 CCRB) after at least a 6h fast.
• After resting quietly in the laboratory for approximately 30min, Dr. Horowitz will collect a resting blood sample by venipuncture, as he has done in our other IRB approved protocols (e.g., HUM00029179). Briefly, Dr. Horowitz will wash his hands and put on gloves. The participant's skin will be disinfected at the venipuncture site with isopropyl alcohol. Single-use needles will be used for each participant and immediately discarded into a biohazard sharps container after use. After the blood draw, direct pressure will be applied to the site of needle insertion to avoid/reduce bruising and discomfort.

• Subjects will exercise (bike or treadmill) for 1h at a moderate exercise intensity (approximately 65% of their predicted VO2peak). This moderate exercise intensity has been used extensively by Dr. Horowitz's laboratory in previous exercise testing and training studies in both lean and obese individuals, and is well tolerated by both populations.

  • During the exercise session, subjects will wear a heart rate monitor to ensure participants are exercising at the correct exercise intensity.
  • Subjects will also use the mouthpiece and nose clip (described above) for ~5min at the beginning, middle, and end of exercise to verify they are exercising at approximately 65% of their predicted VO2peak.
  • A study team member will be present with the participant at all times during exercise to ensure the comfort and safety.
• Immediately after exercise another blood sample will be collected.

• Study Type: Interventional
• Enrollment (Actual): 29
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Michigan
    • Ann Arbor, Michigan, United States, 48109
      • University of Michigan

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Age: 18-40
• Body Mass Index: 20-35 kg/m2
• Women must have regularly occurring menses and must be premenopausal

Exclusion Criteria:

• Pregnant or lactating
• Evidence/history of cardiovascular or metabolic disease
• Medications known to affect lipid or glucose metabolism

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Other: Exercise session; All subjects will exercise for 1 hour at a moderate intensity. There are no different arms in this study	Other: Exercise session; There is no drug or device intervention in this study. Subjects will exercise at a moderate intensity for 60 minutes and a blood sample will be obtained before and after the exercise session

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
changes in plasma concentrations of epinephrine after a session of exercise	Plasma levels will be measured using appropriate commercially available assays.	1 day
changes in plasma concentrations of lactate after a session of exercise	Plasma levels will be measured using appropriate commercially available assays.	1 day
changes in plasma concentrations of fatty acids after a session of exercise	Plasma levels will be measured using appropriate commercially available assays.	1 day
changes in plasma concentrations of IL-6 after a session of exercise	Plasma levels will be measured using appropriate commercially available assays.	1 day
changes in plasma concentrations of irisin after a session of exercise	Plasma levels will be measured using appropriate commercially available assays.	1 day
changes in plasma concentrations of growth hormone after a session of exercise	Plasma levels will be measured using appropriate commercially available assays.	1 day

Collaborators and Investigators

• Sponsor: University of Michigan

Study record dates

Study Major Dates

• Study Start: October 1, 2015
• Primary Completion (Actual): May 11, 2016
• Study Completion (Actual): May 11, 2016

Study Registration Dates

• First Submitted: October 13, 2015
• First Submitted That Met QC Criteria: October 16, 2015
• First Posted (Estimate): October 20, 2015

Study Record Updates

• Last Update Posted (Actual): March 21, 2022
• Last Update Submitted That Met QC Criteria: March 18, 2022
• Last Verified: March 1, 2022

More Information

Terms related to this study

• Other Study ID Numbers: HUM00103937