Protein Supplementation and Recovery of Muscle Function

September 25, 2017 updated by: Phil Jakeman, University of Limerick

The Effect of Milk Derived Protein Supplementation on Muscle Recovery Following Resistance Exercise

The focus is performance nutrition. Resistance exercise can induce low level muscle damage in conjunction with impaired contractile function. Milk-derived proteins contain, or induce, bioactive properties that assist muscle recovery and restore/improve muscle function. The aim of the research is the recovery of muscle function following resistance exercise.

In this study, the investigators propose to undertake a comparison of the ingestion of two milk-derived protein-based recovery drinks on muscle function after resistance exercise compares to an isonitrogenous, non-essential amino acid control.

Study Overview

Detailed Description

Study Design: A block randomised design of 3 groups of resistance-exercise trained (RET) men

Participants: 24 young, healthy, resistance exercise trained, males aged 18 - 35 years.

Recovery drinks; CONTROL - an isonitrogenous, non-essential amino acid solution; PROTEIN - a milk-based whey protein concentrate (WPC), WPC-80; HYDROLYSATE - a hydrolysed derivative of WPC-80.

Day 1, Pre-screening consisting of:

  • medical history and examination by a clinician
  • blood sample to be evaluated for health-related contraindications
  • body composition measurement by dual energy x-ray absorptiometry (DXA) to determine whole body and segmental lean tissue mass;
  • exercise training log (6 month recall);
  • dietary consultation;
  • habitual physical activity level assessed by European Physical Activity Questionaire (EPAQ)-2

Day 2 - 8: Participants will be "free-living" during this time. Participants will record dietary intake under the guidance of a qualified dietitian and sports nutritionist.

Day 9 and 10: Participants will complete two familiarisation sessions to the resistance exercise and muscle function testing procedures.

A single blood sample (5ml) will be drawn following o/n fast on each day. On day 2 participants will drink a metered dose of deuterated water (around 200ml) containing deuterium. A saliva sample will be taken before and after consuming this bolus.

Day 11-17. During this experimental phase diet is prescribed and provided. The diet is based on habitual food intake standardized to body mass.

Day 11: O/n fasted blood, saliva and 1st micro-biopsy of muscle obtained; Muscle function measured prior to and upon completion of a prescribed bout of resistance exercise training (RET); The recovery drink (0.33 g protein/kg body mass) is ingested; 3 hours following completion of RET a 2nd micro-biopsy of muscle is obtained.

Day 12: O/n fasted blood and saliva is obtained Muscle function measured and recovery drink (0.33 g protein/kg body mass) consumed.

Day 13: O/n fasted blood and saliva is obtained Muscle function measured followed by the 2nd RET bout and recovery drink (0.33 g protein/kg body mass) consumed.

Day 14: O/n fasted blood and saliva is obtained Muscle function measured and recovery drink (0.33 g protein/kg body mass) consumed.

Day 15: O/n fasted blood and saliva is obtained Muscle function measured followed by the 3rd RET bout and recovery drink (0.33 g protein/kg body mass) consumed.

Day 16 O/n fasted blood, saliva and 3rd micro-biopsy of muscle obtained Muscle function measured and recovery drink (0.33 g protein/kg body mass) consumed.

Day 17: O/n fasted blood and saliva is obtained Muscle function measured

Study Type

Interventional

Enrollment (Anticipated)

24

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

    • Co Limerick
      • Limerick, Co Limerick, Ireland
        • Recruiting
        • University of Limerick
        • Contact:
        • Contact:
        • Sub-Investigator:
          • Dick Fitzgerald, PhD

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

18 years to 35 years (ADULT)

Accepts Healthy Volunteers

Yes

Genders Eligible for Study

Male

Description

Inclusion Criteria:

  • risk
  • no known current illness or physically active
  • resistance-exercise trained
  • no known intolerance to milk-based products
  • no known blood born disease or medication that would adversely affect participation
  • no adverse risk to venepuncture

Exclusion Criteria:

• recent musculoskeletal injury

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: BASIC_SCIENCE
  • Allocation: RANDOMIZED
  • Interventional Model: FACTORIAL
  • Masking: DOUBLE

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
SHAM_COMPARATOR: CONTROL

Intervention: Dietary Supplement: Non-essential amino acids A group of subjects ingesting a liquid beverage (per kilogram body mass: 0.33g non-essential amino acids dissolved in water ) prior to completion of a prescribed resistance exercise training (RET) session.

Muscle Contractile Function to be conducted prior to and following the RET session by laboratory-based ergometry.

Muscle Protein Synthesis induced by RET to be measured by deuterium incorporation in to skeletal muscle sampled by microbiopsy.

Food grade mixture of non-essential amino acids in powdered form, flavoured and instantised to be dissolved in water.

Supplied by Carbery Food Ingredients Limited, Ballineen, Ireland. Product Reference Number 10955

ACTIVE_COMPARATOR: PROTEIN

Intervention: Dietary Supplement: Whey Protein Concentrate A group of subjects ingesting a liquid beverage (per kilogram body mass: 0.33g of Whey Protein Concentrate dissolved in water ) prior to completion of a prescribed resistance exercise training (RET) session.

Muscle Contractile Function to be conducted prior to and following the RET session by laboratory-based ergometry. Muscle Protein Synthesis induced by RET to be measured by deuterium incorporation in to skeletal muscle sampled by microbiopsy.

Whey protein concentrate in powdered form, flavoured and instantised to be dissolved in water.

Supplied by Carbery Food Ingredients Limited, Ballineen, Ireland. Trade name of product is Carbelac80 Instant Product Reference Number 10431

Other Names:
  • Carbelac80 Instant
ACTIVE_COMPARATOR: HYDROLYSATE

Intervention: Dietary Supplement: Whey Protein Hydrolysate A group of subjects ingesting a liquid beverage (per kilogram body mass: 0.33g of Whey Protein Hydrolysate dissolved in water ) prior to completion of a prescribed resistance exercise training (RET) session.

Muscle Contractile Function to be conducted prior to and following the RET session by laboratory-based ergometry. Muscle Protein Synthesis induced by RET to be measured by deuterium incorporation in to skeletal muscle sampled by microbiopsy.

Whey protein concentrate hydrolysate (degree of hydrolysate 27%) in powdered form, flavoured and instantised to be dissolved in water.

Supplied by Carbery Food Ingredients Limited, Ballineen, Ireland. Product Reference Number 10468

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Change in Muscle Contractile Function
Time Frame: Day 9, 10, 11, 12, 13, 14, 15, 16 and 17
Maximal Voluntary Contract (MVC) of Knee Extensor Muscle measured by laboratory-based ergometry
Day 9, 10, 11, 12, 13, 14, 15, 16 and 17

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Change in Muscle Protein Synthesis
Time Frame: Day 11 and 16
The fractional rate of muscle protein synthesis (MPS) measured by deuterium incorporation into skeletal muscle sampled by microbiopsy
Day 11 and 16

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (ACTUAL)

July 1, 2017

Primary Completion (ANTICIPATED)

September 30, 2017

Study Completion (ANTICIPATED)

December 30, 2017

Study Registration Dates

First Submitted

August 17, 2017

First Submitted That Met QC Criteria

September 25, 2017

First Posted (ACTUAL)

September 29, 2017

Study Record Updates

Last Update Posted (ACTUAL)

September 29, 2017

Last Update Submitted That Met QC Criteria

September 25, 2017

Last Verified

September 1, 2017

More Information

Terms related to this study

Other Study ID Numbers

  • FHIHAPN3.3RX
  • TC20130001 (OTHER_GRANT: Enterprise Ireland)

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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