- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT03334201
Age-associated Arterial Dysfunction, Western Diet, and Aerobic Exercise: Role of the Gut Microbiome
October 27, 2021 updated by: Douglas Seals, University of Colorado, Boulder
The risk of developing cardiovascular diseases increases with aging largely due to age-related declines in the function of arteries (arterial dysfunction), which are influenced by common lifestyle factors such as consumption of a "Western" diet and lack of sufficient physical activity; thus, it is important to study how these factors interact to affect artery function.
This study will determine whether changes to the gut microbiome (the collection of bacteria and other "microbes" living in the intestinal tract) with aging, Western diet consumption and aerobic exercise influence arterial function, and the biological pathways (mechanisms) involved.
Specifically, the investigators will perform a randomized, single-blind, controlled feeding crossover study design (comparing Western diet to non-Western diet consumption) in young and older, exercising and non-exercising healthy adults to determine the time course effects (temporal association) of diet on the gut microbiome and arterial function.
Overall, this research has potential to establish the gut microbiome as a possible target for treating/preventing age-related arterial dysfunction and reducing the risk of age-associated cardiovascular diseases.
Study Overview
Status
Recruiting
Conditions
Intervention / Treatment
Detailed Description
In order to investigate how age-related changes to the gut microbiome, as modulated by Western diet and exercise, influences arterial function in humans, the investigators will capitalize on the plasticity of the gut microbiome to dietary perturbations.
A randomized, single-blind, 2x1-week dietary intervention crossover study (with 3 week washout) will be conducted in young (18-29 years) and old (60-79 years), exercising and non-exercising, male and female adults from all races and ethnic backgrounds.
Testing will take place on the University of Colorado Boulder main campus at the Clinical and Translational Research Center (CTRC - located in the Wardenburg Health Center).
Subjects will undergo telephone and in-person CTRC screening, with those eligible assigned to either Group A or Group B using block randomization stratified by age and exercise status.
Group A will undergo isocaloric WD feeding (high fat [40%], high sugar [25%], low fiber [15g], low nutrient) during Phase 1 (first 1-week treatment period) and non-WD feeding (low-fat [25%], low sugar [15%], high fiber [22g], high nutrient) during Phase 2 (second 1-week treatment period).
Group B will undergo the dietary interventions in the opposite order.
Phases 1 and 2 will be separated by a 3-week washout period to prevent carry over effects.
During each 1-week intervention period, fecal swabs will be collected daily to characterize the gut microbiome and vascular function, will be measured at baseline and after 2 and 7 days of controlled feeding to gain insight into the temporal relation between these outcomes.
Study Type
Interventional
Enrollment (Anticipated)
120
Phase
- Not Applicable
Contacts and Locations
This section provides the contact details for those conducting the study, and information on where this study is being conducted.
Study Contact
- Name: Kara Lubieniecki, MS
- Phone Number: 303-735-6410
- Email: ipalab@colorado.edu
Study Contact Backup
- Name: Vienna E Brunt, PhD
- Phone Number: 303-735-4936
- Email: vienna.brunt@colorado.edu
Study Locations
-
-
Colorado
-
Boulder, Colorado, United States, 80309
- Recruiting
- University of Colorado Boulder
-
Contact:
- Kara Lubieniecki, MS
- Phone Number: 303-735-6410
- Email: ipalab@colorado.edu
-
Contact:
- Vienna E Brunt, PhD
- Phone Number: 303-735-4936
- Email: vienna.brunt@colorado.edu
-
-
Participation Criteria
Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.
Eligibility Criteria
Ages Eligible for Study
18 years to 79 years (Adult, Older Adult)
Accepts Healthy Volunteers
Yes
Genders Eligible for Study
All
Description
Inclusion Criteria:
- Age 18-29 or 60-79 years
- Participating in < 30 min aerobic exercise < 2x/wk for >3 years OR > 45 min vigorous aerobic exercise > 4x/wk for > 3 yrs
- Currently consuming levels of dietary fat intake with the 40th-60th percentile of Americans based on the NHANES survey (29-37% calories from fat)
- Healthy (no clinical disease)
- Body mass index < 30 kg/m2
- Ankle-brachial blood pressure index > 0.9
- Total cholesterol < 240 mg/dl
- Fasting plasma glucose < 110 mg/dl
- Weight stable in the past 6 months
Exclusion Criteria:
- Current or past (within 3 months of screening) use of dietary supplements, anti-inflammatory medications, drugs known to affect the gut microbiome (antibiotics, pre/probiotics, antifungals, antivirals, antiparasitics), or gastrointestinal-targeted drugs (proton pump inhibitors, H2 receptor antagonists, laxatives, antidiarrheal medications)
- Current of past (within 5 years) smoking
- Major change in health status in the last 6 months
- Chronic clinical diseases (e.g., inflammatory bowl diseases, cardiovascular disease, diabetes, neurological disorders, dementia or other brain diseases of aging)
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Design Details
- Primary Purpose: Basic Science
- Allocation: Randomized
- Interventional Model: Crossover Assignment
- Masking: Single
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Other: Western diet first
To receive Western diet controlled feeding first, followed by non-Western diet controlled feeding
|
high fat (40%), high sugar (25%) and low fiber (15g/day)
low fat (25%), low sugar (15%), high fiber (30g/day)
|
Other: Non-Western diet first
To receive non-Western diet controlled feeding first, followed by Western diet controlled feeding
|
high fat (40%), high sugar (25%) and low fiber (15g/day)
low fat (25%), low sugar (15%), high fiber (30g/day)
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change in the gut microbiome
Time Frame: 7 days
|
Sterile fecal swabs will be collected and analyzed using 16S rRNA sequencing
|
7 days
|
Change in endothelial function
Time Frame: 7 days
|
Brachial artery flow-mediated dilation
|
7 days
|
Change in arterial stiffness
Time Frame: 7 days
|
Carotid-femoral pulse wave velocity
|
7 days
|
Collaborators and Investigators
This is where you will find people and organizations involved with this study.
Sponsor
Collaborators
Study record dates
These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.
Study Major Dates
Study Start (Actual)
March 15, 2018
Primary Completion (Anticipated)
December 31, 2021
Study Completion (Anticipated)
December 31, 2021
Study Registration Dates
First Submitted
November 1, 2017
First Submitted That Met QC Criteria
November 2, 2017
First Posted (Actual)
November 7, 2017
Study Record Updates
Last Update Posted (Actual)
October 28, 2021
Last Update Submitted That Met QC Criteria
October 27, 2021
Last Verified
October 1, 2021
More Information
Terms related to this study
Other Study ID Numbers
- 16-0633
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
UNDECIDED
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
No
Studies a U.S. FDA-regulated device product
No
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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