Dental Fluorosis Risk by the Use of Acidulated Fluoride Dentifrice

Dental Fluorosis Risk by the Use of Acidulated Fluoride Dentifrice: Clinical Trial - Epidemiological Study With Children Residing in an área Without Public Water Fluoridation

This research aimed to evaluate the risk of dental fluorosis by using an experimental fluoridated acidulated dentifrice compared to conventional dentifrices in two-to-four-year-old children residing in a non fluoridated area by the concentration of fluoride incorporated in childrens' nails, as well as the incorporation of F into the biofilm. Two hundred and ten children participated on this study. They were randomly allocated into three groups differing according to the type of dentifrice used over 12 months: G1:1100 ppm F, pH 4.5; G2:750 ppm F, pH 4.5; G3:1100 ppm F, pH 7.0. The dentifrice was placed on the toothbrush using the "drop" technique. The toenails of the biggest toe were collected in three moments, corresponding to the experimental period of use of the dentifrices (T1, T2 and T3), as well as the biofilm which was collected twice, 5 and 60 minutes after brushing. The nails and biofilm fluoride concentration were analyzed on a specific electrode using the HMDS diffusion facilitated technique.

Study Overview

• Status: Completed
• Conditions: Caries, Dental; Fluorosis, Dental
• Intervention / Treatment: Other: Use of Dentifrice Fluoride

Detailed Description

This is a randomized, double-blind, interventional trial, adopting a strategy for collecting data, collecting data from the children's biofilm. This study was carried out in three Child Education Reference Centers (CREIs) located in different points of the city of João Pessoa PB.

The city is located in the Northeast region of Brazil, on the coast of the state of Paraíba and has about 600 thousand inhabitants (IBGE, 2013). João Pessoa has already had fluoridated water for a period of two years until the mid-1980s, but so far the project has been deactivated.

The 452 children participating in the study were divided into three groups according to the dentifrice used in a 12 - month period: G1: 1,100 ppm F - pH 4.5; G2: 750 ppm F-pH 4.5; and G3: 1100 ppm F-pH 7.0. The toothpaste was supplied by Oralls (São Jose dos Campos-SP, Brazil) and dental brushes by Bitufo (Hypermarcas, São Paulo, SP-, Brazil) and there was no conflict of interest. For the random distribution of the groups, the CREIs were considered as units, so that only one type of toothpaste was distributed in each CREI, thus facilitating the control of the dentifrices delivery by the teachers, as well as the supervised brushing in CREI itself.

The toenails of the biggest toe were collected in three moments, corresponding to the experimental period of use of the dentifrices (T1, T2 and T3), as well as the biofilm which was collected twice, 5 and 60 minutes after brushing. The nails and biofilm fluoride concentration were analyzed on a specific electrode using the HMDS diffusion facilitated technique.

Nails of the large toes were collected because they provide a sufficient nail mass for analysis and because there has been a reported possibility of contamination of the toenails (Buzalaf, Pessan et al., 2006). The nail samples of each child were cleaned with deionized water using a dental brush, taken to the ultrasound apparatus with deionized water for 10 min, dried at 60 ± 5 ° C and weighed on the analytical balance The presence of F was analyzed as described in the previous item, according to the method of Taves (1968), as modified by Whitford (1996). F patterns (0.00475, 0.0095, 0.019, 0.095, 0.190 and 0.95 mg F) were prepared by serial dilution of a stock solution of 0.1 MF (Orion) in triplicate and diffused in the same manner as the samples. The average repeatability of the readings based on duplicate samples was 95%. Whenever the sample weight was> 20 mg, the analysis was performed in duplicate.

• Study Type: Interventional
• Enrollment (Actual): 452
• Phase: Phase 4

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 2 years to 4 years (CHILD)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Age group of 2 to 4 years of age of both sexes
• Who were enrolled in one of the 3 CREIs that participated in the research
• Presence of deciduos teeth

Exclusion Criteria:

• Presented high mobility of the children (incompatible with the planning of a longitudinal study)
• Children who did not allow the clinical examination in the school
• Children using orthodontic appliances or children with extensive caries lesions with dentin sensitivity
• Children not residing in the city of João Pessoa-PB from birth to 4 years due to the fluoridation of water from public supply.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: TREATMENT
• Allocation: RANDOMIZED
• Interventional Model: PARALLEL
• Masking: QUADRUPLE

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
EXPERIMENTAL: Dentifrice 750 ppm of NaF acidulated; Use of Dentifrice Fluoride Use of few of dentifrice on brushing for 12 months	Other: Use of Dentifrice Fluoride; Use of few of dentifrice on brushing during a year
ACTIVE_COMPARATOR: Dentifrice 1.100 ppm of NaF neutral; Use of Dentifrice Fluoride Use of few of dentifrice on brushing for 12 months	Other: Use of Dentifrice Fluoride; Use of few of dentifrice on brushing during a year
ACTIVE_COMPARATOR: Dentifrice 1.100 ppm of NaF acidulated; Use of Dentifrice Fluoride Use of few of dentifrice on brushing for 12 months	Other: Use of Dentifrice Fluoride; Use of few of dentifrice on brushing during a year

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Concentration of intraoral fluoride	Effective of capacity to concentration of fluoride in biofilm (ppm)	1 year
Effect of intraoral fluoride - software	Reduction of initial lesions carie give by mm² analysed by software	1 year
Effect of intraoral fluoride	Reduction of initial lesions carie give by visual scale (Nyvad scale)	1 year
Concentration of nail fluorid	Concentration of fluoride in nail (ppm)	1 year

Collaborators and Investigators

• Sponsor: Federal University of Paraíba

Study record dates

Study Major Dates

• Study Start (ACTUAL): January 15, 2015
• Primary Completion (ACTUAL): January 15, 2018
• Study Completion (ACTUAL): January 15, 2018

Study Registration Dates

• First Submitted: August 25, 2018
• First Submitted That Met QC Criteria: November 30, 2018
• First Posted (ACTUAL): December 3, 2018

Study Record Updates

• Last Update Posted (ACTUAL): December 3, 2018
• Last Update Submitted That Met QC Criteria: November 30, 2018
• Last Verified: November 1, 2018

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Chemically-Induced Disorders; Stomatognathic Diseases; Tooth Demineralization; Tooth Diseases; Poisoning; Dental Caries; Fluorosis, Dental; Fluoride Poisoning; Physiological Effects of Drugs; Protective Agents; Cariostatic Agents; Fluorides
• Other Study ID Numbers: Acidulated fluoride dentifrice

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No