Investigation of the Effects of Sedentary Behaviour and Moderate Exercise on Glucose Tolerance and Insulin Sensitivity

Individuals with pre-diabetes or diabetes would benefit from low impact methods that would improve their insulin sensitivity and aid in maintaining glucose homeostasis. Physical activity helps the body decrease its insulin resistance and burn excess sugar. Many diabetics also suffer from obesity and specific forms or durations of physical exercise may not be viable options for these individuals. Determining whether short bursts of moderate exercise improve blood glucose levels in healthy humans may identify a further method for diabetics to improve their glucose homeostasis.

The aim of this study is to determine the most effective moderate exercise that can be completed in 3 minutes and its effectiveness on improving glucose handling, in response to a single day of standardized high-fat and high-carbohydrate feeding in comparison to periods of prolonged sitting.

Study Overview

• Status: Terminated
• Conditions: Sedentary Lifestyle; Insulin Sensitivity; Glucose Metabolism
• Intervention / Treatment: Other: Activity/Moderate Exercise

• Study Type: Interventional
• Enrollment (Actual): 6
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Canada
  • Prince Edward Island
    • Charlottetown, Prince Edward Island, Canada, C1A 4P3
      • University of Prince Edward Island

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 50 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• healthy
• BMI between 18-30 kg/m²
• non-smoker

Exclusion Criteria:

• pregnant
• diabetes
• kidney disease
• liver disease
• cardiovascular disease
• cancer

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
No Intervention: Control; No intervention. Study participants will be seated during the entire sedentary 3-hour time period and wheeled to phlebotomy (and exercise) stations when required. During the sedentary period, participants will eat the food according to the study protocol and be seated at desks and allowed to read and use computers.
Experimental: Intervention; Will disrupt their sedentary time with 3 minute exercise sessions every 30 minutes	Other: Activity/Moderate Exercise; 3-hour sedentary period with 3 minute activity breaks of moderate exercise every 30 minutes

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in Insulin levels	The change in blood insulin levels from baseline (12 hours fasted) to 3-hours post-prandial will be determined from blood serum using a human metabolic hormone multiplex panel (Luminex xMAP technology).	Pre-prandial baseline (12 hours fasted) and 3 hours post-prandial on each of two testing days
Change in concentration of C-peptide	The change in blood C-peptide levels from baseline (12 hours fasted) to 3-hours post-prandial will be determined from blood serum using a human metabolic hormone multiplex panel (Luminex xMAP technology).	Pre-prandial baseline (12 hours fasted) and 3 hours post-prandial on each of two testing days
Changes in blood glucose levels	Blood glucose levels will be determined at pre-prandial baseline and over the following 3-hour testing period using hand-held blood glucose meters.	Baseline and every 30-60 minutes over 3 hours (6 timepoints per test day)
Change in metabolomic response to a test meal	Targeted metabolomics analysis of approximately 139 metabolites in blood serum by LC-MS/MS to generate a metabolomic profile in response to a high-fat and high-carbohydrate test meal. Metabolites to be determined are categorized as biogenic amines, amino acids, histidines, carboxylic acids, acylcarnitines, glycerophospholipids and organic acids.	Pre-prandial and 3-hours post test meal through study completion

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Immediate effects of short-term sedentary behaviour on cognitive function	Assessing cognitive abilities using the Stroop Test.	3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Immediate effects of short-term sedentary behaviour on cognitive function	Assessing cognitive abilities using the Iowa Trail Making Test.	3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Immediate effects of short-term sedentary behaviour on cognitive function	Assessing cognitive abilities using the Digit-Span test.	3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Immediate effects of short-term sedentary behaviour on cognitive function	Assessing cognitive abilities using the N-back test.	3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Immediate effects of short-term sedentary behaviour on cognitive function	Assessing cognitive abilities using the Flankers test.	3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Gene expression levels in isolated PBMC populations	Determining changes in gene expression in pathways associated with RAGE signalling.	Pre-prandial and 3 hours post-prandial on on test days through study completion

Collaborators and Investigators

• Sponsor: University of Prince Edward Island
• Investigators: Study Director: Magdalena Mahlstedt, PhD, University of Prince Edward Island

Study record dates

Study Major Dates

• Study Start (Actual): January 17, 2019
• Primary Completion (Actual): October 31, 2019
• Study Completion (Actual): December 31, 2019

Study Registration Dates

• First Submitted: January 22, 2019
• First Submitted That Met QC Criteria: January 31, 2019
• First Posted (Actual): February 1, 2019

Study Record Updates

• Last Update Posted (Actual): January 29, 2021
• Last Update Submitted That Met QC Criteria: January 26, 2021
• Last Verified: January 1, 2019

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Glucose Metabolism Disorders; Metabolic Diseases; Immune System Diseases; Hyperinsulinism; Hypersensitivity; Insulin Resistance
• Other Study ID Numbers: 6007895

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No