Microarchitecture of the Augmented Bone Following Sinus Elevation (MAB)

Biomaterials in the Surgical Rehabilitation of Oral and Maxillofacial Hard and Soft Tissue Defects

In this prospective randomized clinical study sinus floor elevation procedures are preformed using different biomaterials with the lateral window technique. After a 6-month healing, at the time of dental implant placement bone core biopsy samples are harvested. The samples undergo micro-Ct and histomorphometric analysis.

Study Overview

• Status: Completed
• Conditions: Alveolar Bone Loss; Edentulous; Alveolar Process, Atrophy; Sinus Pneumatization
• Intervention / Treatment: Procedure: sinus floor elevation; Procedure: bone core biopsy; Device: dental implant placement; Diagnostic test: Cone Beam Computed Tomography scans (CBCT)

Detailed Description

Aim of the study The aim of this prospective randomized study is to evaluate the remodeling capacity of albumin impregnated freeze-dried bone allograft in sinus floor augmentation by histomorphometry and micro-CT analysis and to compare it to anorganic bovine xenograft material.

Surgical methods Patients performed oral rinse with 0.2% chlorhexidine solution for 1 min before surgery. Under local anesthesia a full thickness flap was raised from a midcrestal incision along with two relieving incisions. Lateral window osteotomies were carried out with diamond burs. The Schneiderian membrane was carefully elevated. Bone graft material was packed in the defect with light force. Patients were randomly assigned to 2 groups based on the type of bone filler: albumin impregnated allograft (BoneAlbumin, OrthoSera GmbH, Krems an der Donau, Austria) or anorganic bovine bone mineral (ABBM), (Bio-Oss Geistlich Wolhusen, Switzerland). A porcine collagen membrane (Bio Gide, Geistlich GmbH, Wolhusen, Switzerland) was placed over the lateral window and fixed by titanium pins (Titan Pin Set, Ustomed Instruments Ulrich Storz GmbH & Co. KG, Tuttlingen, Germany). The buccal flap was mobilized to allow tension-free primary closure. The margins were stabilized with single interrupted sutures. Antibiotics (1 g amoxicillin-clavulanate twice a day for 5 days, or in case of side effects or known allergy to penicillin, 300 mg clindamycin 4 times a day for 4 days), anti-inflammatory drugs (50 mg diclofenac 3 times a day for 3 days), and chlorhexidine mouthwash (twice a day for 21 days from the first day after surgery) were prescribed. Sutures were removed after 10 days. After 6 months healing surgical re-entry procedure took place under local anesthesia. A bone core biopsy was taken with a trephine with an external diameter of 3.5 mm and an internal diameter of 2.5 mm (330 205 486 001 025 Hager & Meisinger GmbH, Neuss, Germany) and implants of at least 4.0 mm of diameter were placed into the grafted alveolus nonsubmerged.

Histomorphometry Bone core biopsy material was fixed in 10% buffered formaldehyde solution. Following decalcination and dehydration, the biopsy material was embedded in paraffin and 20 µm sections were prepared. The sections were stained with routine haematoxylin eosin stain. Sections were evaluated under a light microscope in magnification 40×-400× .

Histomorphometric measurements were completed on sections with Panoramic Viewer 1.15 (3DHISTECH Ltd., Budapest, Hungary) using a combination of Adobe PhotoShop (Adobe System Inc., San Jose, CA, USA) and ImageJ, the public domain NIH Image program (US National Institutes of Health' http://rsb.info.nih.gov/nih-image/) at 150× magnification. Two slides of the augmented areas in each bone core biopsy sample were evaluated to record area percentage of newly formed bone, residual particles of the bone graft material, and the bone marrow space according to published protocols. Histomorphometric data consisted of the area percentage of newly formed bone, residual particles of the graft material and bone marrow were identified in each section.

Micro-CT analysis The bone core biopsy samples were scanned using a microcomputed tomography (μCT) scanner (Skyscan 1172 X-ray microtomograph, Bruker µCT, Kontich, Belgium). After the acquisition, raw images were reconstructed using NRecon software (v.1.7.1.6., Bruker µCT, Kontich, Belgium). The morphometric variables relevant to our study calculated by CTAn software (v.1.17.7.2, Bruker µCT, Kontich, Belgium). On the reconstructed images of each sample the demarcation plane of the host and the augmented area was identified and the complete available tissue (maxilla or augmented bone, respectively) were selected as regions of interest (ROIs) for quantitative analysis.

Micromorphometric data was collected by evaluation of the augmented bone of the test or control group. Additional micromorphometric data was recorded by analyzing the native bone of the alveolar ridge within the bone core biopsy samples of both test and control group. The microarchitectural parameters of the augmented bone of the BoneAlbumin group and the ABBM group; the augmented bone of the BoneAlbumin group and the native bone of the alveolar ridge; the augmented bone of the ABBM group and the native bone of the alveolar ridge were compared.

Statistical analysis Percentage values of each bone core biopsy sample were used to calculate descriptive statistics for the histomorphometrical and quantitative µCT analysis. The results were analyzed statistically using the IBM SPSS Statistics 23 data analysis software program (IBM Corporation, New York, NY. USA). The Kruskal Wallis One-way ANOVA test was used to compare two sets of data for the statistical analysis of the histomorphometric results. One-way ANOVA test was used to compare three sets of data for the statistical analysis of the micromorphometric results of the micro-CT. Values of p < 0.05 were considered statistically significant.

CBCT CBCT imaging (PaX-Reve3D, Vatech, Hwaseong, South-Korea) was carried out prior to sinus floor elevation to evaluate the anatomy and possible pathology of the maxillary sinuses, then 6 months after bone augmentation prior to implant placement (preoperative CBCT), and 3 years after implant placement (postoperative CBCT). To register the preoperative and postoperative CBCT data by anatomical landmarks 3DSlicer 4.10.2 software (The Brigham and Women's Hospital, Inc. Boston, USA) was used. The ROIs were identified by determining the central axis of the implants and placing a virtual cylinder on the preoperative CBCT image sequence with a diameter of 2,5 mm and the length of 8 mm on this axis corresponding with the inner dimensions of the trephine used for bone core biopsy harvesting. The image sequences of selected ROIs were imported in CTAn software (v.1.17.7.2, Bruker micro-CT, Kontich, Belgium) and micromorphometric variables were calculated by the software.

Statistical analysis of CBCT results Correlation of the micromorphometric data obtained from the CBCT images and micro-CT images was determined by The Spearman's rank-order correlation. Statistical analysis was performed using the IBM SPSS Statistics 25 data analysis software program (IBM Corporation, New York, USA). Values of P < 0.05 were considered statistically significant.

• Study Type: Interventional
• Enrollment (Actual): 40
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Hungary
  • Budapest, Hungary, 1088
    • Semmelweis University Department of Community Dentistry

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

• Periodontally healthy patients
• Edentulous posterior maxilla

Exclusion Criteria:

• History of systemic diseases, that alter bone metabolism (osteoporosis, diabetes mellitus),
• History of medication known to alter bone remodelling (bisphosphonates, RANK ligand inhibitor monoclonal antibodies, corticosteroids),
• History of uncontrolled medical or psychiatric disorders,
• Inflammations of the paranasal sinuses or the alveolar process,
• History of tumours or irradiation therapy in the head and neck region,
• Unwillingness to return for follow-ups,
• Pregnancy,
• Smoking,
• Inability to preform proper oral hygiene.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: allograft; The filler used in sinus floor elevation is albumin impregnated allograft.	Procedure: sinus floor elevation; sinus floor elevation with the lateral technique; Other Names: maxillary sinus augmentation; Procedure: bone core biopsy; After 6 month healing time reentry took place and bone core biopsy samples were collected from the augmented bone of the maxillary sinuses with a trephine drill. Biopsy samples were collected from the sites where the dental implants were to be placed.; Device: dental implant placement; During reentry after bone biopsy dental implants were placed.; Diagnostic test: Cone Beam Computed Tomography scans (CBCT); Preoperative CBCT scans are carried out before dental implant placement, 6 months after sinus floor elevations. Postoperative CBCT scans are taken 3 years after sinus floor augmentations.
Experimental: xenograft; The filler used in sinus floor elevation is anorganic bovine bone mineral.	Procedure: sinus floor elevation; sinus floor elevation with the lateral technique; Other Names: maxillary sinus augmentation; Procedure: bone core biopsy; After 6 month healing time reentry took place and bone core biopsy samples were collected from the augmented bone of the maxillary sinuses with a trephine drill. Biopsy samples were collected from the sites where the dental implants were to be placed.; Device: dental implant placement; During reentry after bone biopsy dental implants were placed.; Diagnostic test: Cone Beam Computed Tomography scans (CBCT); Preoperative CBCT scans are carried out before dental implant placement, 6 months after sinus floor elevations. Postoperative CBCT scans are taken 3 years after sinus floor augmentations.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
newly formed bone percentage	newly formed bone area compared to total area in histomorphometry	6 months
BV/TV bone volume fraction	bone volume compared to total volume in micro-CT reconstruction	6 months

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
bone gain	volume of augmented bone on CBCTs	3 years

Collaborators and Investigators

• Sponsor: Semmelweis University
• Investigators: Study Director: György Szabó, Md PhD Msc, Department of Oro-Maxillofacial Surgery and Stomatology Semmelweis University

Study record dates

Study Major Dates

• Study Start (Actual): January 1, 2016
• Primary Completion (Actual): December 31, 2016
• Study Completion (Actual): September 1, 2021

Study Registration Dates

• First Submitted: October 16, 2019
• First Submitted That Met QC Criteria: October 22, 2019
• First Posted (Actual): October 23, 2019

Study Record Updates

• Last Update Posted (Estimated): June 2, 2023
• Last Update Submitted That Met QC Criteria: June 1, 2023
• Last Verified: June 1, 2023

More Information

Terms related to this study

• Keywords: allograft; xenograft; cone beam computed tomography (CBCT); microcomputed tomography (micro-CT); histomorphometry; sinus floor elevation
• Additional Relevant MeSH Terms: Musculoskeletal Diseases; Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Bone Diseases; Periodontal Atrophy; Bone Resorption; Alveolar Bone Loss
• Other Study ID Numbers: 52158-2/2015/EKU [0425/15]

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No