Acute Effects of Vigorous-intensity Aerobic Exercise on Glymphatic Flow in Healthy Adults

Academic Investigational Study of Acute Effects of Vigorous-intensity Aerobic Exercise on Glymphatic Flow in Healthy Adults


Lead Sponsor: Turku University Hospital

Source Turku University Hospital
Brief Summary

This study investigates changes in glymphatic flow in the brain acutely after vigorous-intensity steady-state aerobic exercise. Twenty subjects (10 male and 10 female) perform 25 minute submaximal cycle ergometry exercise and the changes in the glymphatic flow and cerebral perfusion are evaluated using a variety of MRI sequences (e.g. MREG).

Detailed Description

The glymphatic system is a drainage system for the brain to help maintain fluid balance and extract waste metabolites. Glymphatic system function (healthy glymphatic flow) may prevent accumulation of harmful substances and therefore slow down the onset of neurodegenerative diseases.

The hypothesis is, that glymphatic flow could be increased through exercise and enhanced cerebral arterial pulsality, which might explain some of the brain health benefits of exercise proven in epidemiological studies. This study indirectly investigates the acute changes in the glymphatic flow acutely after aerobic exercise using MREG that measures pulse wave propagation as an indirect surrogate marker for glymphatic flow.

Twenty healthy subjects (n=20, 10 male, 10 female, age 18-45 years) will perform 25 minute 70%VO2max cycle ergometry exercise. The glymphatic flow and cerebral perfusion will be measured using magnetic resonance imaging sequences before and after exercise. The glymphatic flow is measured using 10 Hz fMRI technique called magnetic resonance encephalography (MREG). In addition, perfusion MRI is used to evaluate changes in brain perfusion.

Overall Status Not yet recruiting
Start Date August 1, 2020
Completion Date December 31, 2021
Primary Completion Date December 31, 2021
Phase N/A
Study Type Interventional
Primary Outcome
Measure Time Frame
MREG cardiovascular pulse wavefront 3 hours
Secondary Outcome
Measure Time Frame
Cerebral perfusion fMRI 3 hours
Heart rate (HR) 3 hours
Blood pressure (BP) 3 hours
Enrollment 20

Intervention Type: Other

Intervention Name: Cycle Ergometer Exercise

Description: 25 minutes, 70% VO2max steady state cycle ergometry exercise

Arm Group Label: Vigorous-intensity Aerobic Exercise



Inclusion Criteria:

- Age 18-45

- BMI 18-30

- Resting Blood pressure < 140/90 mmHg

Exclusion Criteria:

- History of a cardiac event

- Insulin or medically treated diabetes

- Any chronic disease or condition that could create a hazard to the subject safety, endanger the study procedures or interfere with the interpretation of study results

- Presence of ferromagnetic objects that would make MR imaging contraindicated

- Claustrophobia

- Abundant use of alcohol

- Use of narcotics

- Smoking of tobacco or consuming snuff tobacco

- Diagnosed depressive or bipolar disorder

- Abnormalities in resting ECG (revised by the study physician)

- Pregnancy

Gender: All

Minimum Age: 18 Years

Maximum Age: 45 Years

Healthy Volunteers: Accepts Healthy Volunteers

Overall Official
Last Name Role Affiliation
Eero Rissanen, MD, PhD Principal Investigator Turku University Hospital
Overall Contact

Last Name: Ilkka Heinonen, PhD

Phone: +358 408 393 962

Email: [email protected]

Facility: Turku PET Centre
Location Countries


Verification Date

May 2020

Responsible Party

Type: Principal Investigator

Investigator Affiliation: Turku University Hospital

Investigator Full Name: Ilkka Heinonen

Investigator Title: Adjunct Professor, Department of Clinical Medicine

Has Expanded Access No
Condition Browse
Number Of Arms 1
Arm Group

Label: Vigorous-intensity Aerobic Exercise

Type: Experimental

Description: Vigorous-intensity aerobic exercise, single intervention

Patient Data No
Study Design Info

Allocation: N/A

Intervention Model: Single Group Assignment

Intervention Model Description: Prospective interventional study model, all subjects undergo the same exercise intervention and results are compared to baseline values

Primary Purpose: Basic Science

Masking: None (Open Label)