Effects of a Multi-Ingredient Pre-Workout Supplement on Energy Expenditure During Running

Effects of One Versus Two Doses of a Multi-Ingredient Pre-Workout Supplement on Metabolic Factors and Perceived Exertion During Moderate-Intensity Running in Females

The primary purpose of this study was to examine the acute effects of one versus two doses of a multi-ingredient pre-workout supplement on energy expenditure during moderate-intensity treadmill running. In addition, our second aim was to investigate the responses of associated metabolic factors (i.e. substrate utilization, measures of gas exchange), perceived exertion, and resting cardiovascular variables with one and two doses of the pre-workout supplement.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Dietary supplement: One dose pre-workout condition; Dietary supplement: Two dose pre-workout condition; Dietary supplement: Placebo condition

Detailed Description

This study utilized a randomized, double-blind, placebo-controlled, within-subjects crossover design (Figure 1). Each subject was required to visit the laboratory on five occasions with 72-96 hours between sessions. During the first laboratory visit, each subject performed an incremental test on a treadmill to familiarize the subjects with the testing procedures. For the second laboratory visit, each subject performed an incremental treadmill test to exhaustion to determine their ventilatory threshold (VT). The third laboratory visit was completed in the morning (06:00-09:00) and required subjects to consume a standardized meal after fasting overnight (8 hours) and sat quietly for 30 minutes before baseline heart rate and blood pressure values were recorded. The subjects were then randomly assigned to ingest the supplement (one or two doses) or placebo and sit quietly for another 30 minutes. The ingredients of the supplement (Cellucor, Bryan, TX) are provided in Table 1. The placebo was non-energetic and controlled for similar appearance and taste. At the 15-minute and 30-minute post-ingestion periods (of the pre-workout supplement or placebo), resting heart rate and blood pressure were recorded for a second and third time, respectively. Subjects then performed a 30-minute constant-velocity treadmill run at 90% of their VT. The subjects then returned to the laboratory for their fourth and fifth visits to ingest the remaining substances (1-dose, 2-dose, or placebo) and undergo the same testing procedures (including time of day) as the third visit. Each subject recorded 2-day food logs (MyFitnessPal, Inc., USA) prior to each laboratory visit.

• Study Type: Interventional
• Enrollment (Actual): 12
• Phase: Not Applicable

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 19 years to 29 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Female

Description

Inclusion Criteria:

• ran ≥ 16 km per week

Exclusion Criteria:

• (i) history of medical or surgical events, including cardiovascular disease, metabolic, renal, hepatic, or musculoskeletal disorders; (ii) use of any medication; (iii) use of nutritional supplements; (iv) habitual use of caffeine (≥ one cup of coffee or caffeinated beverage per day); or (v) participation in another clinical trial or investigation of another investigational product within 30 days prior to screening/enrollment.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Double

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: One dose pre-workout supplement condition; One dose/serving of a multi-ingredient pre-workout supplement was administered to the subjects.	Dietary supplement: One dose pre-workout condition; one dose of a multi-ingredient pre-workout supplement
Active Comparator: Two dose pre-workout supplement condition; Two doses/servings of a multi-ingredient pre-workout supplement was administered to the subjects.	Dietary supplement: Two dose pre-workout condition; two doses of a multi-ingredient pre-workout supplement
Placebo Comparator: Placebo condition; One dose/serving of a placebo was administered to the subjects.	Dietary supplement: Placebo condition; one dose of a placebo

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Energy expenditure	energy expenditure measured as kcals per minute	30 minutes of exercise

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
substrate utilization	fat and carbohydrate oxidation	30 minutes of exercise
measures of gas exchange	oxygen consumption, respiratory exchange ratio	30 minutes of exercise
perceived exertion	ratings of perceived exertion (RPE)	30 minutes of exercise
resting cardiovascular variables	heart rate, systolic and diastolic blood pressure	baseline and 30 minutes post-ingestion

Collaborators and Investigators

• Sponsor: Northern Illinois University
• Collaborators: Mayo Clinic; Nova Southeastern University; University of Wisconsin, La Crosse
• Investigators: Principal Investigator: Clayton Camic, PHD, Northern Illinois University

Study record dates

Study Major Dates

• Study Start (Actual): May 16, 2016
• Primary Completion (Actual): May 16, 2016
• Study Completion (Actual): May 16, 2016

Study Registration Dates

• First Submitted: April 16, 2020
• First Submitted That Met QC Criteria: April 16, 2020
• First Posted (Actual): April 20, 2020

Study Record Updates

• Last Update Posted (Actual): April 20, 2020
• Last Update Submitted That Met QC Criteria: April 16, 2020
• Last Verified: April 1, 2020

More Information

Terms related to this study

• Keywords: caffeine; fat oxidation; thermogenic; substrate utilization
• Other Study ID Numbers: NorthernIU

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No