A Natural History of Perioperative Metabolism

The process of surgery is a controlled trauma to the body. Trauma induces changes in metabolic function that have evolved to help the body survive injury. The normal balance among use of sugar, fat, and protein for energy production is thought to change during trauma and surgery. This altered metabolic function may contribute to adverse outcomes from surgical procedures especially in the setting of patients with obesity or Type 2 Diabetes Mellitus. However, very little is known about the specific changes in metabolism that occur during surgical procedures.

The main objective of this project is to describe the metabolic changes that occur during a typical surgical procedure in detail. In order to measure the alterations in the balanced use of sugar, fat, and protein during surgery we will collect blood samples from patients before, during, and after spinal surgical procedures. Subjects will be enrolled in the pre-operative hold area, give informed consent, and have a dedicated peripheral IV catheter placed. We will recruit patients who are normal weight without diabetes, obese without diabetes, and obese with diabetes. The first specific aim is to characterize the metabolic changes in sugar, fat, and protein balance during surgery in metabolically normal subjects. The second specific aim to examine if there are differences in these changes in subjects who are obese or have diabetes. The final specific aim is to measure the changes in metabolism at high resolution using a method called metabolomics, which is analogous to genome profiling. This method measures hundreds of compounds produced in different amounts as metabolic balance changes.

The major impacts that may be derived from these data range from a more thorough understanding of metabolism under trauma to identification of new markers for risk stratification and intervention to improve clinical outcomes. These data will help build the foundation for new approaches to understanding the physiological and metabolic responses to stress and trauma.

Study Overview

• Status: Completed
• Conditions: Surgery; Anesthesia; Insulin Sensitivity; Metabolic Stress Hyperglycemia

Detailed Description

We will conduct a cross-sectional study in a cohort of patients undergoing spinal surgery for discectomy, laminectomy, or vertebral fixation. We will recruit a cohort of subjects in groups with metabolic phenotypes defined by body mass index (BMI) and glycemic control. We are choosing to use spinal surgeries as they utilize inhaled anesthetics, run hours in length, do not involve ischemic tourniquet time, and use basic elements of the anesthetic pharmacopeia including anti-emetics, opioid analgesics, and paralytics. Further the increased surgical stress of entering the thorax or peritoneum are avoided. These features provide a long steady-state of surgical intervention with minimal additional maneuvers allowing for a controlled examination of surgical metabolism.

The categorical criteria for the groups will be body type: lean (BMI<25), overweight (BMI >25, <30), and obese (BMI >30 but <40), and glycemia: normoglycemic (fasting blood glucose; FBG <100 mg/dL or HbA1c <5.5%), impaired fasting glucose (FBG >100 mg/dL and <126 mg/dL, or HbA1c >5.5% and <6.5%), Type 2 Diabetes (FBG >126 mg/dL or HbA1c >6.5%).

Subjects will be screened, enrolled, give informed consent, and have a dedicated peripheral IV line placed in pre-operative hold. A baseline blood sample will be collected in pre-operative hold, after transfer to the table in the operating room, and immediately prior to induction of anesthesia. Following induction blood samples will be collected at 15' intervals for the initial 2 hours of each case, then at 30' intervals for the remainder of the case and for 2 hours in the post-anesthesia care unit. Samples will be processed and stored at -80C until analyzed. Intra-operative data from the anesthesia record will be collected and included in the study database.

• Study Type: Observational
• Enrollment (Actual): 19

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (ADULT, OLDER_ADULT)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

Subjects undergoing multilevel lumbar fusion with an inhaled anesthetic.

Description

Inclusion Criteria:

• Subjects were eligible if they were >18 years old without acute or chronic health conditions who were undergoing multilevel lumbar fusions with an inhaled anesthetic.

Exclusion Criteria:

• acute or chronic health conditions including cardiac, pulmonary, hepatic, renal, auto-immune, or hematological disease. Obese subjects with body mass index >40kg/m2 were excluded. Subjects with Type 2 diabetes were eligible if they were well-controlled (HbA1c <7.5%) and treated with diet and exercise, metformin, or insulin. Subjects were excluded if taking a sulfonylurea, thiazolidindiones, DPP-4 inhibitor, GLP-1 analog, weight loss medication, or other medications affecting glucose homeostasis.

Study Plan

How is the study designed?

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort
surgery; subjects undergoing multilevel lumbar fusion

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
glucose	glucose concentrations	perioperative
insulin	insulin concentrations	perioperative
c-peptide	c-peptide concentrations	perioperative
cortisol	cortisol concentrations	perioperative

Collaborators and Investigators

• Sponsor: University of Vermont Medical Center

Study record dates

Study Major Dates

• Study Start (ACTUAL): April 14, 2014
• Primary Completion (ACTUAL): November 3, 2016
• Study Completion (ACTUAL): July 31, 2019

Study Registration Dates

• First Submitted: May 15, 2020
• First Submitted That Met QC Criteria: May 15, 2020
• First Posted (ACTUAL): May 21, 2020

Study Record Updates

• Last Update Posted (ACTUAL): May 21, 2020
• Last Update Submitted That Met QC Criteria: May 15, 2020
• Last Verified: May 1, 2020

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Glucose Metabolism Disorders; Metabolic Diseases; Hyperinsulinism; Hyperglycemia; Insulin Resistance
• Other Study ID Numbers: M14-291

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No