Comparison of Salivary pH After Consuming Milk of Origin Vegetable vs Cow's Milk in Children

The study was carried out in 21 first grade primary school children, 100% aged 6 years, 52.4% were female. They were given 5 types of milk, each one was measured on different days (Monday cow's milk, Tuesday coconut milk, Wednesday almond milk, Thursday soy milk, Friday rice milk)

Study Overview

• Status: Completed
• Conditions: pH
• Intervention / Treatment: Dietary supplement: Comparison of salivary pH after consuming milk of origin vegetable vs cow's milk

Detailed Description

• The study was carried out in 21 first grade primary school children, 100% aged 6 years, 52.4% were female.
• The group was given a brushing technique using a model and 2 videos were shown to reinforce the brushing technique and explanation of what was going to be done in the study.
• Supervised tooth brushing was performed in a group of 5 people.
• They were given 5 types of milk, each one was measured on different days (Monday cow's milk, Tuesday coconut milk, Wednesday almond milk, Thursday soy milk, Friday rice milk).
• Each child was given 7 plastic cups each day and asked to pour their saliva into the cup when instructed.
• The samples were taken with pH indicator strips and the result was recorded in personalized tables.

• Study Type: Interventional
• Enrollment (Actual): 21
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Mexico
  • Mexico City, Mexico, 11320
    • UNITEC

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 3 years to 6 years (CHILD)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Boys and girls from 3 to 6 years

Exclusion Criteria

• Boys and girls with allergies
• Children with lactose intolerance

Elimination criteria:

- Boys and girls who do not want to participate at the time of the study

Study Plan

How is the study designed?

Design Details

• Primary Purpose: OTHER
• Allocation: NA
• Interventional Model: SINGLE_GROUP
• Masking: NONE

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
EXPERIMENTAL: pH milk; Group of children who were given the different types of milk and only the pH was measured	Dietary supplement: Comparison of salivary pH after consuming milk of origin vegetable vs cow's milk; They were given 5 types of milk, each one was measured on different days (Monday cow's milk, Tuesday coconut milk, Wednesday almond milk, Thursday soy milk, Friday rice milk).; Other Names: cow´s milk, coconut milk, almond milk, soy milk and rice milk

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Sociodemographic results	The study was carried out in 21 first grade primary school children, 100% aged 6 years, 52.4% were female.	1 hour the sociodemographic data of all the children were taken.
pH results	30 minutes after tooth brushing, each child was asked for the first saliva sample (basal pH measurement). After taking the basal pH, each child was given a glass with 50 ml of the milk to be measured. Saliva samples were measured with pH indicator strips and the result was recorded on custom charts. The pH was taken at 5, 10, 20, 30, 40 and 50 minutes. The normality distribution test (Shapiro-Wilk) was applied to the quantitative variables, with p = 0.0001, so that the data present a non-parametric or free distribution. To assess whether there were differences between the pH of the groups, the Kuskal-Wallis test was applied. For basal pH, p = 0.0001, therefore the null hypothesis is rejected and we conclude that the basal pH level is different in the five milks. Average basal pH: Rice milk 7 Cow milk 6.9 Almond milk 6.6 Soy milk 6.4 Coconut milk 6	The average time of pH evaluation for each milk per child was an hour and a half.

Collaborators and Investigators

• Sponsor: Universidad Tecnologica de Mexico
• Investigators: Principal Investigator: Annia Avalos, Master, Universidad Tecnológica Centroamericana

Publications and helpful links

General Publications

• Vanga SK, Raghavan V. How well do plant based alternatives fare nutritionally compared to cow's milk? J Food Sci Technol. 2018 Jan;55(1):10-20. doi: 10.1007/s13197-017-2915-y. Epub 2017 Nov 2.
• Hettinga K, van Valenberg H, de Vries S, Boeren S, van Hooijdonk T, van Arendonk J, Vervoort J. The host defense proteome of human and bovine milk. PLoS One. 2011 Apr 27;6(4):e19433. doi: 10.1371/journal.pone.0019433.
• Cuppari C, Manti S, Salpietro A, Dugo G, Gitto E, Arrigo T, Sturiale M, Salpietro C. ALMOND MILK: A POTENTIAL THERAPEUTIC WEAPON AGAINST COW'S MILK PROTEIN ALLERGY. J Biol Regul Homeost Agents. 2015 Apr-Jun;29(2 Suppl 1):8-12.

Study record dates

Study Major Dates

• Study Start (ACTUAL): January 13, 2020
• Primary Completion (ACTUAL): January 17, 2020
• Study Completion (ACTUAL): January 17, 2020

Study Registration Dates

• First Submitted: January 12, 2021
• First Submitted That Met QC Criteria: January 15, 2021
• First Posted (ACTUAL): January 22, 2021

Study Record Updates

• Last Update Posted (ACTUAL): January 22, 2021
• Last Update Submitted That Met QC Criteria: January 15, 2021
• Last Verified: January 1, 2021

More Information

Terms related to this study

• Other Study ID Numbers: 2019-100

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO
• IPD Plan Description: We are working on the article to publish it and for the study to be available.

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No