Bioavailability of Proteins From Plant Based Diets

Use of Stable Isotopic Dilution to Assess Protein Digestibility From Fava Bean and to Evaluate Amino Acid Bioavailability

Sponsors

Lead Sponsor: International Atomic Energy Agency

Collaborator: AgroParisTech
Institut National de la Recherche Agronomique
Centre National De L'énergie, Des Sciences Et Techniques Nucléaires

Source International Atomic Energy Agency
Brief Summary

Proteins play an important role as structural and functional components for the human body and prevent stunting. Protein quality has a great importance in meeting the nutritional needs of populations across the world throughout the life course, particularly during pregnancy and early childhood. Dietary protein deficiency is mainly due to protein indigestibility limiting indispensable amino acids (IAAs) bioavailability. In Morocco, as it's the case in many developing countries, diet is mainly based on a large consumption of cereals, fruits, vegetables and pulses, and is generally low in meat, which is the main source of protein and IAA. The general objective of this study is to evaluate the digestibility of Fava bean proteins and bioavailability of IAA using a dual-tracer method with stable isotopes.

Detailed Description

This study aims to develop stable isotope labeled protein of bean cultures that could be used for other local common foods, to quantify dietary indispensable amino acids from beans made available to the body for optimal growth, development and function and to participate in the development of a simple method to assess protein quality in vulnerable populations. The project is divided in two parts: the first part of harvesting and production of deuterium labeled fava beans. The optimization test done the first year has shown that the best protocol for labelling was a pulse with 25% D2O followed with subsequent labeling with 2.5% D2O during 20 days. The second part is the intervention stuy that is going to be conducted on 6 healthy volunteers (3 males, 3 females). Each participant will receive a test meal containing 13C labelled spirulina, 2H labelled Fava bean and 13C phenylalanine. The test meal will be divided on 11 subdoses: 3 sub-doses will be administrated at T0 and 1 subdose will be administrated at 1, 2, 3, 4, 5, 6 and 7h. The remaining sub-dose will be kept for measurement of IAA in the test meal. After eating the test meal, blood, urine and breath samples will be collected from each participant at the baseline (T0) and after 5-6, 7h and 8h.

Overall Status Completed
Start Date 2019-11-25
Completion Date 2020-02-15
Primary Completion Date 2019-12-31
Study Type Observational
Primary Outcome
Measure Time Frame
Relative Amino Acid Digestibility One year
DIAAS One year
Secondary Outcome
Measure Time Frame
Scientific articles By the end of 2020
Enrollment 5
Condition
Intervention

Intervention Type: Dietary Supplement

Intervention Name: Use of stable isotopic dilution to assess protein digestibility from Faba bean and to evaluate amino acid bioavailability

Description: Each participant will receive a test meal containing 13C labelled spirulina, 2H labelled Fava bean and 13C phenylalanine. The test meal will be divided on 11 subdoses: 3 sub-doses will be administrated at T0 and 1 subdose will be administrated at 1, 2, 3, 4, 5, 6 and 7h. The remaining sub-dose will be kept for measurement of IAA in the test meal. After eating the test meal, blood, urine and breath samples will be collected from each participant at the baseline (T0) and after 5-6, 7h and 8h.

Arm Group Label: Adults

Eligibility

Sampling Method:

Non-Probability Sample

Criteria:

Inclusion Criteria: Healthy, non-pregnant adults 18-65 years of age Not taking any medication, including NSAIDs Not taking antibiotics within 4 weeks of study BMI within 18-35 range Stable weight Exclusion Criteria: Children and elderly (over 65) Pregnancy Diagnosed with non-communicable or communicable disease Being under restrictive diet Antibiotics within 4 weeks of study Medications within 4 weeks of study

Gender:

All

Minimum Age:

18 Years

Maximum Age:

65 Years

Healthy Volunteers:

Accepts Healthy Volunteers

Overall Official
Location
Facility: Joint Research Unit in Nutrition and Food
Location Countries

Morocco

Verification Date

2021-04-01

Responsible Party

Type: Principal Investigator

Investigator Affiliation: International Atomic Energy Agency

Investigator Full Name: Khayour Meriam

Investigator Title: Principal investigator

Keywords
Has Expanded Access No
Condition Browse
Arm Group

Label: Adults

Description: Inclusion criteria: Healthy, non-pregnant women, adults aged 18-65 years, not taking any medication, including NSAIDs, not taking antibiotics within 4 weeks of study, BMI within 18-35 range. Exclusion criteria: Children and elderly (over 65), pregnant women, individuals diagnosed with non-communicable or communicable disease, being under restrictive diet, antibiotics within 4 weeks of study, medications within 4 weeks of study.

Patient Data Undecided
Study Design Info

Observational Model: Case-Only

Time Perspective: Cross-Sectional

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