Hydrogen-Infused Water for Endurance Performance, Body Hydration Biomarkers, and Health-Related Quality of Life (HYDRO2EAU)

The Effects of Hydrogen-Infused Water on Endurance Performance, Body Hydration Biomarkers, and Health-Related Quality of Life in Healthy Young Men and Women (HYDRO2EAU): A Randomized-Controlled Clinical Trial

The study investigates the impact of 4-week hydrogen-infused water consumption on various health and performance outcomes. Conducted with healthy young adults, the trial assesses endurance performance, hydration biomarkers, and health-related quality of life. Participants are randomized into intervention and control groups, with outcomes measured over a specified period. The findings aim to elucidate whether hydrogen-infused water water provides significant benefits for physical performance, hydration status, and overall well-being compared to standard hydration methods.

Study Overview

• Status: Completed
• Conditions: Exercise Overtraining
• Intervention / Treatment: Dietary supplement: Hydrogen-infused water; Dietary supplement: Placebo

• Study Type: Interventional
• Enrollment (Actual): 10
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Serbia
  • Vojvodina
    • Novi Sad, Vojvodina, Serbia, 21000
      • FSPE Applied Bioenergetics Lab

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Age 18.0 - 29.9 years
• Body mass index 18.5 - 24.9 kg/m2
• Collegiate athletes with training experience >3 years
• Weekly exercise > 5 hours
• Given written informed consent

Exclusion Criteria:

• Major chronic diseases or acute disorders
• History of dietary supplement usage in the 4 weeks preceding the study initiation
• Unwillingness to return for a follow-up
• Participation in other clinical trials

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Hydrogen-infused water; One can of hydrogen-infused water consumed before breakfast and dinner	Dietary supplement: Hydrogen-infused water; Water containing molecular hydrogen
Placebo Comparator: Placebo; One can of hydrogen-free water consumed before breakfast and dinner	Dietary supplement: Placebo; Water containing no molecular hydrogen

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Time to exhaustion	Running time to exhaustion at treadmill	Change from baseline time to exhaustion at 4 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Total body water	Level of total body water	Change from baseline total body water at 4 weeks

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
36-Item Short Form Health Survey	Short Form Health Survey total score, minimum and maximum possible score of between 0 and 100, with higher scores mean better outcome	Change from baseline 36-Item Short Form Health Survey total score at 4 weeks

Collaborators and Investigators

• Sponsor: University of Novi Sad, Faculty of Sport and Physical Education

Publications and helpful links

General Publications

• Ostojic SM. Hydrogen Gas as an Exotic Performance-Enhancing Agent: Challenges and Opportunities. Curr Pharm Des. 2021;27(5):723-730. doi: 10.2174/1381612826666200922155242.

Study record dates

Study Major Dates

• Study Start (Actual): November 15, 2024
• Primary Completion (Actual): February 28, 2025
• Study Completion (Actual): April 15, 2025

Study Registration Dates

• First Submitted: January 16, 2025
• First Submitted That Met QC Criteria: January 16, 2025
• First Posted (Actual): January 22, 2025

Study Record Updates

• Last Update Posted (Actual): December 22, 2025
• Last Update Submitted That Met QC Criteria: December 15, 2025
• Last Verified: December 1, 2025

More Information

Terms related to this study

• Keywords: endurance
• Additional Relevant MeSH Terms: Fatigue; Pathological Conditions, Signs and Symptoms; Signs and Symptoms; Overtraining Syndrome
• Other Study ID Numbers: THIC-1-24

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: YES
• IPD Plan Description: Data obtained through this study may be provided to qualified researchers with academic interest in hydrogen research in biomedicine. Data will be coded, with no PHI included. Approval of the request and execution of all applicable agreements are prerequisites to the sharing of data with the requesting party.
• IPD Sharing Supporting Information Type: STUDY_PROTOCOL; SAP; ICF; CSR

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No