A Comparison of Fisetin Kinetics in Young and Old Adults (FISEKIN-1)

A Comparison of Fisetin Kinetics in Young and Old Adults - FISEKIN-1

This study investigates the differences in the kinetic parameters of fisetin in two cohorts of healthy volunteers:

Cohort 1) volunteers aged 18-30 years (n = 40) Cohort 2) volunteers aged 65 years or older (n = 40)

The purpose of this study is:

1. To describe the fisetin kinetics after a single dose oral administration in older age.
2. To compare the fisetin kinetics after a single dose oral administration in old and young age.

FISEKIN-1 is designed as a four-arm study protocol. As well as two different age groups (18-30 years vs. 65 years and older), we want to compare fisetin kinetic parameters in fasted and fed condition:

Arm 1) 500 mg fisetin (1x 5 capsule), fasted condition, cohort 1: young age Arm 2) 500 mg fisetin (1x 5 capsule), fasted condition, cohort 2: old age Arm 3) 500 mg fisetin (1x 5 capsule), fed condition, cohort 1: young age Arm 4) 500 mg fisetin (1x 5 capsule), fed condition, cohort 2: old age

Study Overview

• Status: Recruiting
• Conditions: Pharmacokinetic Study in Healthy Volunteers
• Intervention / Treatment: Dietary supplement: Fisetin - Fasted condition; Dietary supplement: Fisetin - Fed condition

Detailed Description

The study is designed as an open-label, two-cohorts (young vs. old), four-arm, cross-over, single oral dose protocol.

A single oral dose of fisetin will be administered as capsules with 240 ml of still water in the overnight fasting condition (arm 1; 2) or in fed condition (arm 3; 4).

The arms will be conducted in each participant in random order with a wash-out period of at least one week between each arm.

Fed condition (arm 3; 4): We will serve a high-caloric and high-fat meal based on the recommendation of the FDA (Food and Drug Administration) for food-effect studies. This meal will contain two slices of toast with butter, two eggs fried in butter, approx. 113 g hash brown potatoes and 240 ml whole milk.

A total of 24 blood samples will be taken at defined time points (baseline; 10; 20; 30; 40; 50; 60; 80; 100 min; 2.0; 2.5; 3.0; 3.5; 4.0; 4.5; 5.0; 5.5; 6.0; 6.5; 7.0; 7.5; 8.0; 9.0; 10.0 h). At each time point, blood will be collected (4.9 ml for separation of plasma) to determine fisetin, and its metabolites.

The total amount of blood collected for each participant and each arm is 164 ml at the kinetic visits and 12 ml at the screening visit.

After intake of fisetin, participants will drink 200 ml of sparkling water every hour to stimulate gastrointestinal peristalsis and to promote transport of the capsule. After 2 hours, the participants may drink a cup of tea or coffee and after 4 hours they will be served a meal low in fisetin content. Urine will be collected during the first 10 hours after fisetin administration. Monitoring of blood pressure and heart rate will take place for the first 4 hours after administration. Volunteers will stay in the Clinical Research Unit of the Institute of Pharmacology for the first 10 hours after administration.

• Study Type: Interventional
• Enrollment (Estimated): 80
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Stefan Engeli, Prof.; Phone Number: +49 3834 86 56 33; Email: stefan.engeli@med.uni-greifswald.de

Study Locations

• Germany
  • Greifswald, Germany
    • Recruiting
    • Department of Clinical Pharmacology, Institute of Pharmacology at the Center of Drug Absorption and Transport (C_DAT), University Medicine Greifswald
    • Contact: Stefan Engeli, Prof.; Phone Number: +49 3834 86 56 33; Email: stefan.engeli@med.uni-greifswald.de

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• any sex
• age between 18 and 30 years or ≥ 65 years
• understands the study purpose and design
• contractually capable and provides signed informed consent form
• healthy condition or mild and/or well-treated forms of allergies, asthma, hypertension, and orthopedic diseases
• a maximum of 3 chronically taken drugs

Exclusion Criteria:

• BMI > 30 kg/m2 and < 18 kg/m2
• body weight < 48 kg
• women in young cohort: known pregnancy or lactation period; positive urine pregnancy test at screening or kinetic visit
• men: hemoglobin < 8,3 mmol/l women: hemoglobin < 7,3 mmol/l
• elevated liver function tests (1 or more of ALAT, ASAT, yGT, Bilirubin > 2x ULN)
• reduced renal function (eGFRMDRD < 60 ml/min/1,7 m2)
• QTcF > 450 ms in screening ECG
• psychiatric disease requiring recent or actual treatment
• drug dependency at the time of visit
• use of recreational drugs more than twice a week
• any known hypersensitivity or allergic reactions to fisetin
• history of severe hypersensitivity reactions and/or anaphylaxis
• poor venous conditions that make it impossible to place a peripheral venous catheter and regularly draw blood through it
• intake of drugs undergoing extensive metabolism via CYP1A2, CYP2D6, CYP2C8, CYP2C9, CYP2C19, CYP3A4, and/or Pgp and with narrow (monitoring-requiring) therapeutic range during the past seven days if the duration of intake was at least two days
• intake of COMT-inhibitors during the past seven days if the duration of intake was at least two days
• intake of drugs or dietary supplements containing fisetin within the two days before or during the kinetic visits
• individuals who have eaten food with high fisetin content in the two days before the kinetic visits (e.g. strawberry, apple, persimmon, grape, mango, kiwi, peach, tomato, onion, lotus roots, kale, cucumber; processed products, e.g. wine)

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: Arm 2: Fisetin, fasted condition - Cohort 2: old age (≥ 65 years)	Dietary supplement: Fisetin - Fasted condition; A single oral dose of 500 mg fisetin (as Novusetin®) will be administered as 5 capsules with 240 ml of still water in the overnight fasting condition: A total of 24 blood samples will be taken at defined time points (baseline; 10; 20; 30; 40; 50; 60; 80; 100 min; 2.0; 2.5; 3.0; 3.5; 4.0; 4.5; 5.0; 5.5; 6.0; 6.5; 7.0; 7.5; 8.0; 9.0; 10.0 h). At each time point, blood will be collected (4.9 ml for separation of plasma) to determine fisetin, and fisetin metabolites. The total amount of blood collected for each participant and each arm is 164 ml at the kinetic visits and 12 ml at the screening visit. Urine will be collected during the first 10 hours after fisetin administration.
Active Comparator: Arm 4: Fisetin, fed condition - Cohort 2: old age (≥ 65 years)	Dietary supplement: Fisetin - Fed condition; A single oral dose of 500 mg fisetin (as Novusetin®) will be administered as 5 capsules with 240 ml of still water in fed condition: Participants will eat a high-caloric and high-fat meal based on the recommendation of the FDA (Food and Drug Administration) for food-effect studies. This meal will contain two slices of toast with butter, two eggs fried in butter, approx. 113 g hash brown potatoes and 240 ml whole milk. A total of 24 blood samples will be taken at defined time points (baseline; 10; 20; 30; 40; 50; 60; 80; 100 min; 2.0; 2.5; 3.0; 3.5; 4.0; 4.5; 5.0; 5.5; 6.0; 6.5; 7.0; 7.5; 8.0; 9.0; 10.0 h). At each time point, blood will be collected (4.9 ml for separation of plasma) to determine fisetin, and fisetin metabolites. The total amount of blood collected for each participant and each arm is 164 ml at the kinetic visits and 12 ml at the screening visit. Urine will be collected during the first 10 hours after fisetin administration.
Active Comparator: Arm 1: Fisetin, fasted condition - Cohort 1: young age (18-30 years)	Dietary supplement: Fisetin - Fasted condition; A single oral dose of 500 mg fisetin (as Novusetin®) will be administered as 5 capsules with 240 ml of still water in the overnight fasting condition: A total of 24 blood samples will be taken at defined time points (baseline; 10; 20; 30; 40; 50; 60; 80; 100 min; 2.0; 2.5; 3.0; 3.5; 4.0; 4.5; 5.0; 5.5; 6.0; 6.5; 7.0; 7.5; 8.0; 9.0; 10.0 h). At each time point, blood will be collected (4.9 ml for separation of plasma) to determine fisetin, and fisetin metabolites. The total amount of blood collected for each participant and each arm is 164 ml at the kinetic visits and 12 ml at the screening visit. Urine will be collected during the first 10 hours after fisetin administration.
Active Comparator: Arm 3: Fisetin, fed condition - Cohort 1: young age (18-30 years)	Dietary supplement: Fisetin - Fed condition; A single oral dose of 500 mg fisetin (as Novusetin®) will be administered as 5 capsules with 240 ml of still water in fed condition: Participants will eat a high-caloric and high-fat meal based on the recommendation of the FDA (Food and Drug Administration) for food-effect studies. This meal will contain two slices of toast with butter, two eggs fried in butter, approx. 113 g hash brown potatoes and 240 ml whole milk. A total of 24 blood samples will be taken at defined time points (baseline; 10; 20; 30; 40; 50; 60; 80; 100 min; 2.0; 2.5; 3.0; 3.5; 4.0; 4.5; 5.0; 5.5; 6.0; 6.5; 7.0; 7.5; 8.0; 9.0; 10.0 h). At each time point, blood will be collected (4.9 ml for separation of plasma) to determine fisetin, and fisetin metabolites. The total amount of blood collected for each participant and each arm is 164 ml at the kinetic visits and 12 ml at the screening visit. Urine will be collected during the first 10 hours after fisetin administration.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Fisetin plasma concentration arm 1 vs. arm 2	Difference of fisetin plasma concentrations between the young and old cohort, expressed as area under the curve (AUC0-10h).	10 hours
Fisetin plasma concentration arm 3 vs. arm 4	Difference of fisetin plasma concentrations between the young and old cohort, expressed as area under the curve (AUC0-10h).	10 hours

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Fisetin metabolites plasma concentration arm 1 vs. arm 2	Difference in plasma concentrations of fisetin metabolites between the young and old cohort, expressed as area under the curve (AUC0-10h).	10 hours
Fisetin metabolites plasma concentration arm 3 vs. arm 4	Difference in plasma concentrations of fisetin metabolites between the young and old cohort, expressed as area under the curve (AUC0-10h).	10 hours
Fisetin plasma concentration arm 1 vs. arm 2	Difference plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as maximum plasma concentration (Cmax).	10 hours
Fisetin plasma concentration arm 3 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as maximum plasma concentration (Cmax).	10 hours
Fisetin plasma concentration arm 1 vs. arm 2	Difference in plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as time at maximum concentration (Tmax).	10 hours
Fisetin plasma concentration arm 3 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as time at maximum concentration (Tmax).	10 hours
Fisetin plasma concentration arm 1 vs. arm 2	Difference in plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as total clearance.	10 hours
Fisetin plasma concentration arm 3 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as total clearance.	10 hours
Fisetin plasma concentration arm 1 vs. arm 2	Difference in plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as renal clearance.	10 hours
Fisetin plasma concentration arm 3 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as renal clearance.	10 hours
Fisetin plasma concentration arm 1 vs. arm 2	Difference in plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as apparent volume of distribution.	10 hours
Fisetin plasma concentration arm 3 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between the young and old cohort, expressed as apparent volume of distribution.	10 hours
Fisetin plasma concentration arm 1 vs. arm 3	Difference of fisetin plasma concentrations between arm 1 and arm 3, expressed as area under the curve (AUC0-10h).	10 hours
Fisetin plasma concentration arm 2 vs. arm 4	Difference of fisetin plasma concentrations between arm 2 and arm 4, expressed as area under the curve (AUC0-10h).	10 hours
Fisetin plasma concentration arm 1 vs. arm 3	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 1 and arm 3, expressed as maximum plasma concentration (Cmax).	10 hours
Fisetin plasma concentration arm 2 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 2 and arm 4, expressed as maximum plasma concentration (Cmax).	10 hours
Fisetin plasma concentration arm 1 vs. arm 3	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 1 and arm 3, expressed as time at maximum concentration (Tmax).	10 hours
Fisetin plasma concentration arm 2 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 2 and arm 4, expressed as time at maximum concentration (Tmax).	10 hours
Fisetin plasma concentration arm 1 vs. arm 3	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 1 and arm 3, expressed as total clearance.	10 hours
Fisetin plasma concentration arm 2 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 2 and arm 4, expressed as total clearance.	10 hours
Fisetin plasma concentration arm 1 vs. arm 3	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 1 and arm 3, expressed as renal clearance.	10 hours
Fisetin plasma concentration arm 2 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 2 and arm 4, expressed as renal clearance.	10 hours
Fisetin plasma concentration arm 1 vs. arm 3	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 1 and arm 3, expressed as apparent volume of distribution.	10 hours
Fisetin plasma concentration arm 2 vs. arm 4	Difference in plasma concentrations of fisetin and fisetin metabolites between arm 2 and arm 4, expressed as apparent volume of distribution.	10 hours

Collaborators and Investigators

• Sponsor: University Medicine Greifswald

Study record dates

Study Major Dates

• Study Start (Estimated): March 31, 2026
• Primary Completion (Estimated): June 30, 2027
• Study Completion (Estimated): July 31, 2027

Study Registration Dates

• First Submitted: January 13, 2025
• First Submitted That Met QC Criteria: January 21, 2025
• First Posted (Actual): January 28, 2025

Study Record Updates

• Last Update Posted (Actual): April 6, 2026
• Last Update Submitted That Met QC Criteria: March 31, 2026
• Last Verified: March 1, 2026

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Physiological Effects of Drugs; Molecular Mechanisms of Pharmacological Action; Antioxidants; Protective Agents; Quercetin
• Other Study ID Numbers: IPHA-2025-010

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No