Inflammatory Responses After CrossFit and Resistance Trainings in Untrained Subjects

The Effect of a CrossFit Training Program on Physical, Physiological, and Hormonal Responses in Untrained Subjects

Considering the remarkable increase of the fitness programs, the purpose of this study was to assess and compare the effect of Crossfit (CF) and periodized resistance (RT) trainings on inflammatory/oxidative responses in untrained individuals.

Twenty-four participants (20 men and 4 women, age= 25.7 ± 2.2 years, BMI= 25.7 kg.m-2) were randomly assigned into 12 weeks of these trainings with a rate of five sessions/week. Before and after intervention, participants anthropometric data and graded exercise tests were performed. Before (pre), immediately after (post) and 60min after test (p60) a 20mL venous blood sample were drawn for a later measure of c-reactive protein (CRP), interleukin 1 beta (IL-1ß), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), thiobarbituric acid reactive substances (TBARS), antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx).

Study Overview

• Status: Completed
• Conditions: Inflammation; Oxidative Stress; Hormones; Plasma Volume
• Intervention / Treatment: Behavioral: 12 week training; Behavioral: 12 week training

• Study Type: Interventional
• Enrollment (Actual): 24
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Tunisia
  • Ariana Governorate
    • Aryanah, Ariana Governorate, Tunisia, 2073
      • Institut Supérieur du Sport et de l'Education Physique de Ksar-Saïd

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Child; Adult; Older Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Participants must be non-smokers,
• Participants should not suffer from respiratory issues
• Participants should have healthy kidney and liver
• Participants have no metabolic and neurological disorders

Exclusion Criteria:

• The use of medications during the previous 4 weeks
• Consumption of any product related to anti-inflammatory/oxidative supplements

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: CrossFit; High intensity functional training	Behavioral: 12 week training; Anti-inflammatory and oxidative environment following 12-weeks of CF training by evaluating inflammatory markers and oxidative precursors in untrained participants; Behavioral: 12 week training; Anti-inflammatory and oxidative environment following 12-weeks of resistance training by evaluating inflammatory markers and oxidative precursors in untrained participants
Experimental: Resistance; Periodized resistance training	Behavioral: 12 week training; Anti-inflammatory and oxidative environment following 12-weeks of CF training by evaluating inflammatory markers and oxidative precursors in untrained participants; Behavioral: 12 week training; Anti-inflammatory and oxidative environment following 12-weeks of resistance training by evaluating inflammatory markers and oxidative precursors in untrained participants

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Inflammatory marker	c-reactive protein (CRP),	Participants data were performed in one day before beginning the 12 week intervention and one day after the last session
Inflammatory marker	interleukin 1 beta (IL-1ß)	Participants data were performed in one day before beginning the 12 week intervention and one day after the last session
Inflammatory marker	tumor necrosis factor alpha (TNF-α)	Participants data were performed in one day before beginning the 12 week intervention and one day after the last session
Inflammatory marker	interleukin 6 (IL-6)	Participants data were performed in one day before beginning the 12 week intervention and one day after the last session
Inflammatory marker	interleukin 10 (IL-10)	Participants data were performed in one day before beginning the 12 week intervention and one day after the last session
Oxidative stress	thiobarbituric acid reactive substances (TBARS)	Participants data were performed in one day before beginning the 12 week intervention and one day after the last session
Oxidative stress	antioxidant enzymes catalase (CAT)	Participants data were performed in one day before beginning the 12 week intervention and one day after the last session
Oxidative stress	glutathione peroxidase (GPx)	Participants data were performed in one day before beginning the 12 week intervention and one day after the last session

Collaborators and Investigators

• Sponsor: Habil Hamdouni

Study record dates

Study Major Dates

• Study Start (Actual): November 8, 2021
• Primary Completion (Actual): February 8, 2022
• Study Completion (Actual): April 17, 2022

Study Registration Dates

• First Submitted: September 14, 2025
• First Submitted That Met QC Criteria: September 18, 2025
• First Posted (Estimated): September 24, 2025

Study Record Updates

• Last Update Posted (Estimated): September 24, 2025
• Last Update Submitted That Met QC Criteria: September 18, 2025
• Last Verified: September 1, 2025

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Pathologic Processes; Pathological Conditions, Signs and Symptoms; Inflammation
• Other Study ID Numbers: ISS-1386

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: UNDECIDED
• IPD Plan Description: Under negotation

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No