Significance of Biological Markers in Patients With Acute Lung Injury/Acute Respiratory Disease

Prognostic Significance of Biological Markers in Patients With ALI/ARDS

The purpose of this study is to identify biological markers of disease in patients with acute lung injury (ALI) that are predictive of either disease susceptibility or prognosis, or that identify novel targets of therapeutic intervention.

Study Overview

• Status: Completed
• Conditions: Respiratory Distress Syndrome, Adult
• Intervention / Treatment: Procedure: Bronchoscopy

Detailed Description

BACKGROUND:

Respiratory failure due to ALI and acute respiratory distress syndrome (ARDS) remains a major health problem despite significant progress in intensive care unit (ICU) care and ventilator management. It is also characterized by an unacceptably high mortality rate despite enormous expenditure of health care resources. Survivors of respiratory failure face long-term consequences concerning their quality of life. New therapies are needed to improve early survival and to decrease long-term sequelae of this syndrome. The purpose of this study is to identify biological markers of disease in patients with ALI that are predictive of either disease susceptibility or prognosis, or that identify novel targets of therapeutic intervention.

DESIGN NARRATIVE:

As soon as possible after case identification, informed consent will be obtained from the patient or next of kin. Physiologic measurements and specimen collection will begin at the time of entry into the study. The inclusion criteria for this study allow entry of patients who have fulfilled criteria for ALI/ARDS for up to 48 hours. Bronchoalveolar lavage (BAL) fluid and blood will be collected at various times after the onset of ALI/ARDS in order to measure levels of a predetermined set of biological markers. In addition, DNA will be collected from patients and analysed for the presence of specific genetic polymorphisms that might alter either disease susceptibility or clinical expression of disease. The levels of these markers or the presence of specific genetic polymorphisms will be correlated with measure of pulmonary inflammation and extent of lung injury, as defined by: 1) PaO2/FiO2 ratios; 2) lung compliance; 3) plateau pressures; and 4) calculation of the Murray Lung Injury Score (obtained at entry and Days 1, 2, 3, 5, 7,10, 14, and 21). Secondary outcome measures to be directly correlated with biomarker expression will include indicators of maladaptive responses to ALI (including the development of multiple organ dysfunction syndrome [MODS]), fibroproliferation, and nosocomial pneumonia (events which greatly impact the clinical course of patients with ALI/ARDS). Thus, the secondary outcome measures include: 1) the development of organ failure (using the Sequential Organ Failure Assessment [SOFA] score); 2) time on ventilator; 3) ventilator-free days; 4) ICU and hospital length of stay; 5) hospital mortality; 6) development of pneumonia; 7) development of lung fibrosis (as determined by high-resolution computed tomography [HRCT] and pulmonary function testing); and 8) health related and lung-specific quality of life (as assessed with the Medical Outcome Studies 36-Item Short form Health Survey Standard Form [SF-36] and St. George's Respiratory Questionnaire).

• Study Type: Interventional
• Enrollment (Actual): 40
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Georgia
    • Atlanta, Georgia, United States, 30322
      • Emory University
  • Michigan
    • Ann Arbor, Michigan, United States, 48109
      • University of Michigan

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 95 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

Acute onset of illness with:

• PaO2/FiO2 ratio of less than 300 (ALI) or PaO2/FiO2 ratio of less than 200 (ARDS)
• Bilateral infiltrates consistent with pulmonary edema on frontal chest radiograph (infiltrates may be patchy, diffuse, homogeneous, or asymmetric)
• Positive pressure ventilation via an endotracheal tube
• No clinical evidence of left atrial hypertension (if measured, pulmonary arterial wedge pressure less than or equal to 18 mm Hg)
• First three criteria must occur together within a 24-hour interval

Exclusion Criteria:

• Greater than 48 hours elapsed following institution of mechanical ventilation
• Pregnancy

• Chronic respiratory failure as defined by any of the following:

  1. FEV1 less than 20 ml/kg of PBW; FEV1/FVC less than 50%
  2. Chronic hypercapnia or hypoxemia
  3. Hospitalization within past 6 months for acute respiratory failure
  4. Chronic home use of oxygen or mechanical ventilation
• Left ventricular failure as defined by New York Heart Association (NYHA) class IV status
• History of hematological malignancy or bone marrow transplantation
• Entry in other intervention clinical trials
• Decision of the patient or attending physician to forego aggressive care
• Expected survival of less than 6 months (based solely on pre-existing medical problems [e.g., poorly controlled neoplasm or other end-stage disease])
• AIDS (known history of HIV infection)
• Prednisone (or equivalent) therapy of 20 mg/day or more for a period of at least 2 months with treatment continuing within 3 weeks prior to screening
• Cytotoxic therapy within 3 weeks of screening
• Morbid obesity defined as greater than 1 kg/c body weight
• At risk for increased intracranial pressure that may result from permissive hypercapnia
• Permissive hypercapnia that is contraindicated
• Neuromuscular disease that would potentially impact ability to wean from mechanical ventilation
• Receiving extracorporeal membrane oxygenation when meeting screening criteria

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Diagnostic
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
PaO2/FiO2 ratios	Measured at Year 4
Lung compliance	Measured at Year 4
Plateau pressures	Measured at Year 4
Calculation of the Murray Lung Injury Score (obtained at entry and Days 1, 2, 3, 5, 7,10, 14, and 21; analyzed at Year 4)	Measured at Year 4

Secondary Outcome Measures

Outcome Measure	Time Frame
Development of organ failure	Measured at Year 4
Time on ventilator	Measured at Year 4
Ventilator-free days	Measured at Year 4
ICU and hospital length of stay	Measured at Year 4
Hospital mortality	Measured at Year 4
Development of pneumonia	Measured at Year 4
Development of lung fibrosis	Measured at Year 4
Health-related and lung-specific quality of life	Measured at Year 4

Collaborators and Investigators

• Sponsor: University of Michigan
• Collaborators: National Heart, Lung, and Blood Institute (NHLBI)
• Investigators: Study Chair: Theodore J. Standiford, MD, University of Michigan

Study record dates

Study Major Dates

• Study Start: July 1, 2004
• Primary Completion (Actual): April 1, 2009
• Study Completion (Actual): June 1, 2009

Study Registration Dates

• First Submitted: September 19, 2005
• First Submitted That Met QC Criteria: September 19, 2005
• First Posted (Estimate): September 22, 2005

Study Record Updates

• Last Update Posted (Estimate): August 19, 2013
• Last Update Submitted That Met QC Criteria: August 16, 2013
• Last Verified: July 1, 2009

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Respiratory Tract Diseases; Respiration Disorders; Lung Diseases; Infant, Newborn, Diseases; Lung Injury; Infant, Premature, Diseases; Respiratory Distress Syndrome; Respiratory Distress Syndrome, Newborn; Acute Lung Injury
• Other Study ID Numbers: 292; P50HL074024 (U.S. NIH Grant/Contract)