Muscle Training Induced Angiogenesis in COPD (COPDµvasc)

Impact of Exercise Training on Skeletal Muscle Capillary Maturation During Angiogenesis in COPD Patients

COPD patients experiment a peripheral muscle dysfunction which impact their exercise tolerance and health-related quality of life. The capillary to fiber interface is reduced and impact the exercise capacity of the patients. While the muscle capillary creation in response to exercise training is blunted in COPD patients, the maturation of the neo-capillaries could also be blunted and contribute to the impaired aniogionenesis in patients. Because the capillary maturation is a sensitive and dynamic process, only different modalities of exercise training and multiple time-points of measures would allow to capture this microvascular adaptation.

Aim of the study : Compare the muscle capillary maturation in response to training at 5 and 10 weeks, in sedentary healthy subject trained at the intensity of the ventilatory threshold (60-65% of VO2max), versus :- COPD patients trained at a similar intensity (60-65% of VO2max)- COPD patients trained at a similar absolute intensity (90% of VO2max).

Study Overview

• Status: Completed
• Conditions: COPD; Peripheral Muscle Dysfunction
• Intervention / Treatment: Procedure: Muscular biopsy; Other: 5-10 weeks sport training

• Study Type: Interventional
• Enrollment (Actual): 22
• Phase: Not Applicable

Contacts and Locations

Study Locations

• France
  • Montpellier, France, 34295
    • Department of clinical Physiology - University Hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 40 years to 78 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• COPD patients :
• FEV1<80%pred.
• non exacerbation during the past 4 weeks
• no oxygen therapy, -

Healthy subjects:

• <150min/week of moderate-to-vigorous physical activity,
• Voorrips score <9

Exclusion Criteria:

• Unstable comorbidity
• Long term oxygen therapy or ventilation
• Rehabilitation program during the past 12 months
• nutritional supplementation during the past 4 weeks
• allopurinol or N-acetylcystein during the past 4 weeks
• long corticosteroid treatment during the past 6 months
• hyperreactivity to xylocain
• anticoagulant or platelet aggregation inhibitors treatment
• participation to an other research study-pregnancy or impossibility to provide informed consent, ...

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Triple

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: COPD Patients ~90% VO2max	Procedure: Muscular biopsy
Active Comparator: COPD patients ~60-65% VO2max	Procedure: Muscular biopsy
Placebo Comparator: healthy volunteers	Procedure: Muscular biopsy; Other: 5-10 weeks sport training

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps	5 weeks
Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps	10 weeks

Secondary Outcome Measures

Outcome Measure	Time Frame
quadriceps endurance	5 weeks
quadriceps endurance	10 weeks
Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps	5 weeks
Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps	10 weeks
Effect of the serum of COPD patients and SHS on pericyte morphology and in vitro	10 weeks
Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vitro	10 weeks
Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vivo	10 weeks

Collaborators and Investigators

• Sponsor: University Hospital, Montpellier
• Collaborators: Institut National de la Santé Et de la Recherche Médicale, France; Université Montpellier; 5 Santé
• Investigators: Principal Investigator: Farés Gouzi, University Hospital

Publications and helpful links

General Publications

• Souweine JS, Gouzi F, Badia E, Pomies P, Garrigue V, Morena M, Hayot M, Mercier J, Ayoub B, Quintrec ML, Raynaud F, Cristol JP. Skeletal Muscle Phenotype in Patients Undergoing Long-Term Hemodialysis Awaiting Kidney Transplantation. Clin J Am Soc Nephrol. 2021 Nov;16(11):1676-1685. doi: 10.2215/CJN.02390221.

Study record dates

Study Major Dates

• Study Start: January 1, 2014
• Primary Completion (Actual): April 1, 2016
• Study Completion (Actual): August 1, 2016

Study Registration Dates

• First Submitted: January 10, 2014
• First Submitted That Met QC Criteria: January 16, 2014
• First Posted (Estimate): January 20, 2014

Study Record Updates

• Last Update Posted (Actual): January 14, 2020
• Last Update Submitted That Met QC Criteria: January 10, 2020
• Last Verified: January 1, 2020

More Information

Terms related to this study

• Keywords: COPD; angiogenesis; exercise training; skeletal muscle; intensity
• Other Study ID Numbers: 9224