Proteomic Analysis of Mononuclear Cells After a High-Fat, High-Carbohydrate Meal With or Without Orange Juice

Proteomic Analysis of the Peripheral-Blood Mononuclear Cells Response After a High-Fat, High-Carbohydrate Meal With or Without Orange Juice in Healthy Subjects

A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers. The present study will assess the effect of a single HFHC meal taken with water, orange juice or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals. Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals.

Study Overview

• Status: Completed
• Conditions: Inflammation
• Intervention / Treatment: Other: Water; Other: Orange juice; Other: Water with glucose

Detailed Description

BACKGROUND:

A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers.

OBJECTIVES:

To study the effect of a single HFHC meal taken with water, orange juice (500ml) or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals.

DESIGN AND METHODOLOGY:

Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals. Blood glucose, insulin and lipid profile were measured. Also, proteome analysis using TMT labeling was performed on peripheral-blood mononuclear cells collected before and at times 3 and 5h after the meal.

• Study Type: Interventional
• Enrollment (Actual): 12
• Phase: Not Applicable

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 35 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• healthy
• normal weight
• non-vegetarian

Exclusion Criteria:

• use of medication
• alcohol consumption,
• intense physical exercise
• pregnant or lactating
• overweight
• obese
• drug abuse
• any disease such as diabetes, inflammatory conditions.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Placebo Comparator: Water; The subjects will ingest the HFHC meal with 500mL of water.	Other: Water; Individuals will consume the HFHC meal with 500mL of water.
Experimental: Orange juice; The subjects will ingest the HFHC meal with 500mL of orange juice.	Other: Orange juice; Individuals will consume the HFHC meal with 500mL of orange juice.
Active Comparator: Water with glucose; The subjects will ingest the HFHC meal with 500mL of water with glucose (isocaloric control of the orange juice).	Other: Water with glucose; Individuals will consume the HFHC meal with 500mL of water with glucose.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Change in protein expression at mononuclear cells of healthy individuals before and after the high fat meal using TMT labeling and mass spectrometry	before the meal, 1h, 3h and 5h after the meal

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Blood glucose		before the meal
Blood glucose		1h after the meal
Blood glucose		3h after the meal
Blood glucose		5h after the meal
Insulin		before the meal
Insulin		1h after the meal
Insulin		3h after the meal
Insulin		5h after the meal
Lipid profile	total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides	before the meal
Lipid profile	total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides	1h after the meal
Lipid profile	total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides	3h after the meal
Lipid profile	total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides	5h after the meal

Collaborators and Investigators

• Sponsor: University of Sao Paulo
• Collaborators: The Scripps Research Institute
• Investigators: Principal Investigator: Daniela FS Chaves, PhD, University of Sao Paulo

Publications and helpful links

General Publications

• Ghanim H, Sia CL, Upadhyay M, Korzeniewski K, Viswanathan P, Abuaysheh S, Mohanty P, Dandona P. Orange juice neutralizes the proinflammatory effect of a high-fat, high-carbohydrate meal and prevents endotoxin increase and Toll-like receptor expression. Am J Clin Nutr. 2010 Apr;91(4):940-9. doi: 10.3945/ajcn.2009.28584. Epub 2010 Mar 3. Erratum In: Am J Clin Nutr. 2011 Mar;93(3):674. Upadhyay, Mannish [corrected to Upadhyay, Manish].
• Coelho RC, Hermsdorff HH, Bressan J. Anti-inflammatory properties of orange juice: possible favorable molecular and metabolic effects. Plant Foods Hum Nutr. 2013 Mar;68(1):1-10. doi: 10.1007/s11130-013-0343-3.
• Chaves DFS, Carvalho PC, Brasili E, Rogero MM, Hassimotto NA, Diedrich JK, Moresco JJ, Yates JR 3rd, Lajolo FM. Proteomic Analysis of Peripheral Blood Mononuclear Cells after a High-Fat, High-Carbohydrate Meal with Orange Juice. J Proteome Res. 2017 Nov 3;16(11):4086-4092. doi: 10.1021/acs.jproteome.7b00476.

Study record dates

Study Major Dates

• Study Start: June 1, 2015
• Primary Completion (Actual): September 1, 2015
• Study Completion (Actual): October 1, 2015

Study Registration Dates

• First Submitted: October 22, 2015
• First Submitted That Met QC Criteria: October 23, 2015
• First Posted (Estimate): October 27, 2015

Study Record Updates

• Last Update Posted (Estimate): July 29, 2016
• Last Update Submitted That Met QC Criteria: July 27, 2016
• Last Verified: July 1, 2016

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Pathologic Processes; Inflammation
• Other Study ID Numbers: TMTMNC