Capillary Glycerolemia Evolution During Physical Exercise (LSEEGLYCEROL)

Maximum fatty acid oxidation is known to occur at exercise intensities between 45 and 65% VO2max and in the fasting state.

However, the optimal level may depend on various unknown factors. Glycerolemia is a good marker of the fatty acid metabolism but its evolution remains poorly described, maybe due to the difficulty of the measure.

A new device allows an easy measurement of glycerolemia during exercise and could enable personalization strategies to optimize exercise efficacy.

In this study, the investigators plan to test three different exercise types and three different fasting conditions with three replicates each to evaluate the variability of glycerolemia and its evolution during exercise.

Study Overview

• Status: Completed
• Conditions: Physical Activity; Body Mass Index
• Intervention / Treatment: Other: Physical exercice - moderate intensity; Other: Physical exercice - high intensity; Other: Physical exercice - workout; Other: Normal meal; Other: Normalized meal; Other: Fasting

Detailed Description

The metabolism of mobilized fatty acids as an energy reserve during physical exercise produces glycerol, which makes it a marker of the intensity of lipolysis.

This measurement of the intensity of lipolysis in humans can be carried out according to several methods. Since glycerol is a product of lipolysis, its rate of appearance (Ra) in the blood represents a physical quantity naturally associated with lipolysis activity. Unfortunately, its quantification requires the use of a tracer isotope (deuterated glycerol), which limits its applicability. The alternative is the determination of glycerolemia in the blood or in the interstitial fluid via a microdialysis probe placed in subcutaneous adipose tissue;

Recently, a measuring device has been proposed which makes it possible to measure glycerolemia from capillary blood, in the manner of measuring blood glucose in diabetic patients. This device has the advantage of being used repeatedly after a physical exercise. This flexibility also makes it possible to consider the personalization of the sports program by identifying the circumstances associated with an increase in lipolysis such as the type of meal preceding the exercise or its intensity.

Evidence suggests that moderate-intensity exercise (about 45% -65% VO2max) performed under fasting conditions maximizes lipid metabolism as a source of energy, compared with carbohydrate metabolism. But these data conceal a likely significant variability in individual outcomes, including levels of training, gender, age, and nutritional intake prior to exercise. Our hypothesis is that the current recommendation to the diabetic patient, or with a metabolic syndrome, to exercise moderate intensity fasting physical activity could be adapted individually to each patient in order to optimize the benefits of such a practice. In this sense, the easy access to a measurement of capillary glycerolemia could make it possible to identify in a personalized way the type of physical activity.

This research project aims to accurately describe the evolution of capillary glycerolemia in several configurations of physical exercise (variable intensity) by controlling the effect of the previous meal. It will also seek to identify situations associated with a greater increase in capillary glycerolemia to pave the way for personalization of the sports activity program.

• Study Type: Interventional
• Enrollment (Actual): 12
• Phase: Not Applicable

Contacts and Locations

Study Locations

• France
  • Paris, France, 75018
    • Hopital Bichat Claude-Bernard

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion criteria :

• Major patients followed in Diabetology-Nutrition Day Hospital of Bichat-Claude Bernard Hospital as part of the ETAPES program
• overweight or obese patient: body mass index BMI> 25 kg / m²
• Volunteer to participate in and follow a proposed physical activity program as part of regular follow-up
• Volunteer to participate in the research, comfortable with the use of the device of auto measurement of capillary glycerolemia

Exclusion criteria :

• Pregnancy in progress or breastfeeding
• Patient under guardianship or curatorship
• Patient participating in another interventional research protocol
• No affiliation to the social security scheme or French Universal Health Cover (CMU)
• Absence of informed consent, written and signed

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm

Intervention / Treatment

Experimental: Single Arm; Every patient will undergo every combination of physical exercise/meal type (3x3 combinations), one following the other. Each combination is replicated three times.

Other: Physical exercice - moderate intensity; standardized physical activity (moderate intensity) lasting 30 minutes and having a warm-up portion of 5 minutes; Other: Physical exercice - high intensity; standardized physical activity (high intensity) lasting 30 minutes and having a warm-up portion of 5 minutes; Other: Physical exercice - workout; standardized physical activity (bodybuilding) lasting 30 minutes and having a warm-up portion of 5 minutes; Other: Normal meal; - Hyperlipidic meal Energy supply: 800 kcal Distribution of macronutrients: proteins: 15% +/- 5; carbohydrates: 40% +/- 5; lipids: 45 +/- 5 Choice of foods determined with the dietician taking into account the patient's tastes and products available on the market The same meal will be taken before the 3 sessions concerned.; Other: Normalized meal; - Hyperglucidic meal Energy supply: 800 kcal Macronutrient distribution: protein 15% +/- 5; carbohydrates 60% +/- 5; lipids 25 +/- 2 Choice of foods determined with the dietician taking into account the patient's tastes and products available on the market. The same meal will be taken before the 3 sessions concerned.; Other: Fasting; non meal in the hour before exercise

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
concentration of glycerol in capillary blood	The primary endpoint is the time series of measurements of capillary glycerol concentration using the DietSee device (LSEE, Ajaccio, France)	at the beginning of exercise
concentration of glycerol in capillary blood	The primary endpoint is the time series of measurements of capillary glycerol concentration using the DietSee device (LSEE, Ajaccio, France)	20 minutes after the beginning of exercise
concentration of glycerol in capillary blood	The primary endpoint is the time series of measurements of capillary glycerol concentration using the DietSee device (LSEE, Ajaccio, France)	30 minutes after the beginning of exercise
concentration of glycerol in capillary blood	The primary endpoint is the time series of measurements of capillary glycerol concentration using the DietSee device (LSEE, Ajaccio, France)	45 minutes after the beginning of exercise
concentration of glycerol in capillary blood	The primary endpoint is the time series of measurements of capillary glycerol concentration using the DietSee device (LSEE, Ajaccio, France)	60 minutes after the beginning of exercise

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
1. Maximum concentration of glycerol in capillary blood	Maximum capillary glycerolemia (peak) among the time series	from the beginning of the exercise and during one hour
2. Glycerol concentration in peripheral venous blood,	Glycerolemia in peripheral venous blood, measured at the end of a bodybuilding type exercise session	30 minutes after the beginning of bodybuilding exercise (i.e. at the end of the exercice)

Collaborators and Investigators

• Sponsor: Assistance Publique - Hôpitaux de Paris
• Investigators: Principal Investigator: Boris HANSEL, MD PHD, Assistance Publique - Hôpitaux de Paris

Study record dates

Study Major Dates

• Study Start (Actual): October 23, 2019
• Primary Completion (Actual): September 11, 2020
• Study Completion (Actual): September 11, 2020

Study Registration Dates

• First Submitted: August 1, 2019
• First Submitted That Met QC Criteria: September 9, 2019
• First Posted (Actual): September 10, 2019

Study Record Updates

• Last Update Posted (Actual): February 25, 2021
• Last Update Submitted That Met QC Criteria: February 24, 2021
• Last Verified: November 1, 2020

More Information

Terms related to this study

• Other Study ID Numbers: 190089 - IDRCB 2019-A00200-57

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No