- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT05329337
Link Between the Peripheral Mononuclear Cells' Capacity to Induce Insulin Resistance and Hyperinsulinemia (IRACTIV)
Seeking a Link Between the Capacity of Peripheral Mononuclear Cells to Induce Insulin Resistance and the Development of Hyperinsulinemia in a General Population
This is a two-center proof-of-concept study, ancillary to the MetACTIV study, whose objective is to define immune activation profiles from the data of individuals followed by the Caisse Primaire d'Assurance Maladie du Gard (health insurance fund).
The IRACTIV study will include a subset of volunteers from the MetACTIV study for whom a blood sample will be taken as part of the IRACTIV study.
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
The local health insurance fund regularly offers people who have already had a free health check-up the opportunity to re-do a check-up. This invitation will be made as part of the routine, by mail. People who have participated in the MetACTIV study will be asked to participate in the present IRACTIV study ( they will be sent an information note). That way, up to 30 volunteers who previously participated in the MetACTIV study, including 10 for whom a particular profile has been identified will be recruited again in sequence.
On the day of consultation at the Gard health insurance fund, consent will be collected from subjects who have agreed to participate. The participation of the volunteers will be reduced to this single visit and 16 mL of blood will be collected on EDTA for the purpose of this study. As in the previous MetACTIV study, this blood will be transported to the Biological Resource Center at Nîmes University Hospital. From this blood, the plasma will be frozen and stored at -80°C for subsequent determination of insulin levels under the same conditions as for the MetACTIV study. Peripheral blood mononuclear cells will be frozen and, later, cultured at the Institute of Human Genetics in complete medium for 48 hours. The effect of the supernatants of these cultures on Akt phosphorylation in HepG2 cells exposed to 10nM of insulin will then be quantified by two different techniques (Western Blot and ELISA). This effect will be expressed as a percentage of inhibition compared to the phosphorylation of Akt induced by insulin alone. The insulin slope between the measurement performed in the MetACTIV study and this IRACTIV study will also be calculated and compared to the peripheral blood mononuclear cells ability to induce insulin resistance in vitro.
Study Type
Enrollment (Actual)
Phase
- Not Applicable
Contacts and Locations
Study Contact
- Name: Anissa MEGZARI
- Phone Number: +33 4.66.68.42.36
- Email: drc@chu-nimes.fr
Study Contact Backup
- Name: Sophie DE BOUARD, PhD
- Phone Number: +33 4.66.68.42.37
- Email: sophie.debouard@chu-nimes.fr
Study Locations
-
-
Gard
-
Nîmes, Gard, France, 30000
- Centre Hospitalier Universitaire
-
Nîmes, Gard, France, 3000
- Caisse Primaire d'Assurance Maladie du Gard
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Description
Inclusion Criteria:
- Individuals who participated in the MetACTIV study.
- Profile 2 subgroup-specific inclusion criteria:
- MetACTIV study individual with a Profile 2.
- Persons who have given free informed consent.
- Persons who have signed the consent form.
- Persons affiliated to or benefiting from a health insurance plan.
- Adults (≥18 years of age).
Exclusion Criteria:
- Persons participating in Category 1 research involving human subjects
- Persons in an exclusion period as determined by another study.
- Persons under court protection, guardianship or trusteeship.
- Persons who are incapable of giving consent.
- Persons for whom it is impossible to give clear information.
- Pregnant, parturient or breastfeeding woman.
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Health Services Research
- Allocation: Non-Randomized
- Interventional Model: Sequential Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: 10 patients with high insulin levels (13.3 ± 9.2 microU/mL) and markers of metabolic syndrome
These are individuals who were isolated from the previous MetACTIV study due to their specific immune profile (Profile 2) defined by 43 immune activation markers and characterized by a high percentage of differentiated T cells and activated T cells.
|
Sixteen mL of blood will be collected on EDTA for the purpose of this study
|
Active Comparator: 20 patients with other immune profiles
|
Sixteen mL of blood will be collected on EDTA for the purpose of this study
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Fasting blood glucose
Time Frame: Day 0
|
Fasting blood glucose will be measured during a routine health evaluation and measured in mM/L.
|
Day 0
|
End-of-study insulinemia
Time Frame: Month 18
|
The amount of insulin in the blood of patients will be measured during a routine health evaluation and measured in µU/L.
|
Month 18
|
Effect of peripheral blood mononuclear cell supernatant on intensity of the signal induced by insulin in hepatocytes.
Time Frame: Hour 48
|
Peripheral blood mononuclear cells are isolated on a Ficoll gradient and frozen.
These frozen peripheral blood mononuclear cells will be sent to the Institute of Human Genetics for culture and supernatants will be recovered after 48 hours.
The effect of these supernatants on insulin signaling will then be tested.
To do so, they will be added or not to cultured HepG2 cells stimulated 24 hours later by 10nM insulin.
After stimulation, HepG2 cells will be lysed and their total Akt and phosphorylated Akt content measured by ELISA and Western Blot.
The proportion of phosphorylated Akt in HepG2 cells exposed to peripheral blood mononuclear cells supernatant will be compared with the proportion of phosphorylated Akt in HepG2 cells not exposed to peripheral blood mononuclear cells supernatant and expressed as the percentage of insulin signaling inhibition induced by the peripheral blood mononuclear cells supernatant.
|
Hour 48
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
A. Percentage inhibition of Akt phosphorylation in Profile 2 patients compared with other immune profiles.
Time Frame: Hour 48
|
The decrease in signal intensity induced in hepatocytes by insulin induced by peripheral blood mononuclear cell supernatant from volunteers with Profile 2 will be compared to the decrease in this intensity induced by peripheral blood mononuclear cell supernatant from volunteers with another immune activation Profile.
Measured as a percentage.
|
Hour 48
|
B. Increase in insulinemia over time in Profile 2 patients compared with other immune profiles.
Time Frame: Month 18
|
Insulinemia in Profile 2 volunteers, measured in µU/mL, will be compared with that of other immune profiles.
|
Month 18
|
Other Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Age of Profile 2 patients
Time Frame: Day 0
|
In years
|
Day 0
|
Weight of profile 2 patients
Time Frame: Day 0
|
In kg
|
Day 0
|
Gender of Profile 2 patients
Time Frame: Day 0
|
male / female
|
Day 0
|
Waist measurement of Profile 2 patients
Time Frame: Day 0
|
In cm
|
Day 0
|
Hip circumference of Profile 2 patients
Time Frame: Day 0
|
In cm
|
Day 0
|
Systolic blood pressure in Profile 2 patients
Time Frame: Day 0
|
In mmHg
|
Day 0
|
Diastolic blood pressure in Profile 2 patients
Time Frame: Day 0
|
In mmHg
|
Day 0
|
High-density lipoproteins in Profile 2 patients
Time Frame: Day 0
|
mM/L
|
Day 0
|
Low-density lipoproteins in Profile 2 patients
Time Frame: Day 0
|
mM/L
|
Day 0
|
Triglycerides in Profile 2 patients
Time Frame: Day 0
|
mM/L
|
Day 0
|
Hemoglobin A1c in Profile 2 patients
Time Frame: Day 0
|
mM/L
|
Day 0
|
Collaborators and Investigators
Collaborators
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Estimated)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- NIMAO/2020-1/PC-01
- 2021-A03189- 32 (Other Identifier: EudraCT)
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
Clinical Trials on Insulin Resistance
-
Washington University School of MedicineNational Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); National...CompletedEndoplasmic Reticulum Stress | HIV Related Insulin Resistance | Protease Inhibitor Related Insulin ResistanceUnited States
-
Paloma Almeda-ValdésCompleted
-
German Diabetes CenterYale UniversityRecruiting
-
National Institute of Diabetes and Digestive and...Active, not recruitingSevere Insulin ResistanceUnited States
-
Eunice Kennedy Shriver National Institute of Child...Completed
-
Assiut UniversityCompleted
-
National Taiwan UniversityNational Cheng-Kung University HospitalCompletedExercise, Insulin Resistance, Visceral Adipose TissueTaiwan
-
The University of Texas Medical Branch, GalvestonCompletedInsulin Resistance, DiabetesUnited States
-
University Health Network, TorontoCompletedInsulin Resistance, DiabetesCanada
-
University Health Network, TorontoCompletedInsulin Resistance Syndrome X | Pancreatic Beta Cell FunctionCanada
Clinical Trials on Blood test
-
French National Agency for Research on AIDS and...Completed
-
Pascual Gregori RoigHospital Universitario de la Plana; FUNDACIÓN DAVALOS FLETCHERCompletedHyperbilirubinemia, Neonatal | Anemia Neonatal | Polycythemia SecondarySpain
-
Wingate InstituteTel Aviv UniversityCompleted
-
University Hospital, ToursCompleted
-
Cairo UniversityUnknownClass III Malocclusion | Class II Malocclusion
-
Assistance Publique Hopitaux De MarseilleCompleted
-
Imperial College LondonCompletedHereditary Hemorrhagic Telangiectasia | Pulmonary Arteriovenous MalformationsUnited Kingdom
-
Minovia Therapeutics Ltd.RecruitingMyelodysplastic SyndromesIsrael
-
Centre Hospitalier Universitaire de NīmesRecruitingNeoplasm Metastasis | Colorectal Cancer | Disseminated CancerFrance
-
University Hospital, GenevaHospices Civils de LyonNot yet recruitingAbuse, Child | Protein Disorder, BloodFrance, Switzerland