Effects of Collagen Peptide Supplementation on Muscle and Tendon Damage and Function

A Randomized Double-blinded Placebo-controlled Study to Evaluate the Effect of Collagen Peptide (Collagen Peptan) Supplementation on Muscle and Tendon Damage, Repair, and Functional Capacity During Exercise Training Overload

The aim of the study is to investigate the acute and short-term effects of collagen peptides (Collagen Peptan) on muscle and connective tissue during an eccentric overload training period. Currently, all studies investigating the effects of post-exercise collagen supplementation did this in the absence of whey proteins, which are part of the standard recommendations for post-exercise recovery. The investigators will therefore investigate whether the combined intake of whey proteins and collagen peptides ensures a better recovery of exercise performance and whether it can prevent or reduce symptoms of muscle and tendon overload during a three-week eccentric overload training period (n = 22).

Study Overview

• Status: Completed
• Conditions: Healthy Male Subjects; Exercise
• Intervention / Treatment: Dietary supplement: whey protein; Dietary supplement: whey protein + collagen peptide

• Study Type: Interventional
• Enrollment (Actual): 22
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Belgium
  • Vlaams-Brabant
    • Leuven, Vlaams-Brabant, Belgium, 3001
      • Exercise Physiology Research Group

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Sports participation, including general fitness training, between 2 and 5 hours max per week
• Body mass index between 18.5 and 25

Exclusion Criteria:

• Any kind of injury/pathology that is a contra-indication to perform high-intensity exercise - Intake of any medication or nutritional supplement that could impact muscle protein synthesis and/or exercise performance during the period of the study
• Intake of any whey protein, casein of branched-chain amino acid (BCAA) supplements from 1 month prior to the start of the study
• Blood donation within 3 months prior to the start of the study
• Smoking
• Current participation in another research trial
• Any other argument to believe that the subject is unlikely to successfully complete the full study

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Quadruple

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Placebo Comparator: Placebo; Only whey protein will be provided	Dietary supplement: whey protein; Daily 45g of whey protein supplementation during a three week eccentric training period
Experimental: Collagen; A mix of whey protein and collagen peptides will be provided	Dietary supplement: whey protein + collagen peptide; Daily 15g of whey protein and 20g collagen peptide supplementation during a three week eccentric training period

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in countermovement jump performance	Change in jump height when performing a countermovement jump, measured via a force platform	Pretest - 48 hours after the first training session (day 3 of training intervention) - Posttest (day 22 of training intervention)
Change in maximal isometric knee-extension force of the right leg	Change in maximal isometric knee-extension force (angle = 45°) of the right leg	Pretest - 48 hours after the first training session (day 3 of training intervention) - Posttest (day 22 of training intervention)
Change in anteroposterior thickness of the right patellar tendon	Change in anteroposterior thickness of the right patellar tendon	Pretest - 48 hours after the first training session (day 3 of training intervention) - Posttest (day 22 of training intervention)
Change in neovascularization of the right patellar tendon	Neovascularization of the right patellar tendon scored with the Modified Ohberg Score (range 0 - 4; the higher the score, the higher the degree of neovascularization).	Pretest - 48 hours after the first training session (day 3 of training intervention) - Posttest (day 22 of training intervention)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in pain perception at the muscular level (VAS scale)	Change in pain perception at the muscular level of the right leg, measured via the Visual Analogue Scale (range 0 - 10; the higher the score, the higher the pain level)	Training 1 (day 1 of training intervention) - Training 2 (day 3 of training intervention) - Training 8 (day 9 of training intervention) - Training 17 (day 19 of training intervention) - Posttest (day 22 of training intervention)
Change in pain perception of the patellar tendon of the right leg (VISA-P)	Change in pain perception of the patellar tendon of the right leg, measured via the VISA-P questionnaire (range: 0 - 100; the lower the score, the higher the symptoms of patellar tendon overload)	Pretest - Training 1 (day 1 of training intervention) - Training 17 (day 21 of training intervention) - Posttest (day 22 of training intervention)
Change in the blood concentration of creatine kinase	Change in blood concentration of creatine kinase (marker of muscle damage)	Pretest - Training 2 (day 3 of training intervention) - Posttest (day 22 of training intervention)
Change in the blood concentration of Interleukin 6 (IL-6)	Change in blood concentration of Interleukin 6 (IL-6; marker of inflammation)	Pretest - Training 2 (day 3 of training intervention) - Posttest (day 22 of training intervention)
Change in the blood concentration of N-terminal propeptide of type I procollagen (P1NP)	Change in blood concentration of N-terminal propeptide of type I procollagen (P1NP; marker of collagen synthesis)	Pretest - Training 2 (day 3 of training intervention) - Posttest (day 22 of training intervention)

Collaborators and Investigators

• Sponsor: KU Leuven
• Collaborators: Rousselot BVBA

Study record dates

Study Major Dates

• Study Start (Actual): April 12, 2021
• Primary Completion (Actual): May 30, 2021
• Study Completion (Actual): June 30, 2021

Study Registration Dates

• First Submitted: August 3, 2021
• First Submitted That Met QC Criteria: June 16, 2022
• First Posted (Actual): June 21, 2022

Study Record Updates

• Last Update Posted (Actual): June 21, 2022
• Last Update Submitted That Met QC Criteria: June 16, 2022
• Last Verified: June 1, 2022

More Information

Terms related to this study

• Keywords: Whey protein; Collagen peptide; Muscle and tendon overload training
• Other Study ID Numbers: S64402

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: UNDECIDED

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No