Recovery Kinetics Following a Soccer Training in Middle-aged Males

This study aims at investigating the recovery kinetics of skeletal muscle damage, neuromuscular fatigue and performance following a single soccer trainning session in middle aged males. The participants will perform a soccer training session [A single training session including 60 minutes of warm up, soccer technical exercises and small-sided game] and a cotrol trial (No intervention included, only daily measurements) in randomized, repeated measures, crossover design. Assesesments related to skeletal muscle damage, performance and neuromuscular fatigue will be executed before the training session and daily for four consecutive days after training.

Study Overview

• Status: Recruiting
• Conditions: Skeletal Muscle Damage
• Intervention / Treatment: Other: ST

Detailed Description

Eleven male middle-aged participants will be included in this study. Participants will be initially informed about the main goals of study as well as the associated risks and benefits and then will provide their signed consent form. At baseline, they will undergo assessment of their anthropometrics (body mass, height, BMI, WHR), body compotition (using DXA), physical contition level (peak oxygen consumption, VO2peak), haemodynamic profile (diastolic and diastolic blood pressure) and daily dietary intake.Thereafter, they will participate in two experimental trials (Control trial and Soccer trial) in a randomized, crossover, repeated measure design: 1) ST (Soccer Trial): Participants in this trial will perform a soccer training session (60 min) consisted of warm-up, technical exercises and small-sided game, CT (Control Trial): Participants in this trial will only execute the evaluations daily in the dependent variables (they will not receive any intervention). Before each trial, the participants will provide a resting blood sample (for the determination of white blood cells, granulocyte cells, monocyte cells, lymphocyte cells, Hemeatocrit, Hemoglobin, red blood cells and platelets, total antioxidant capacity, glutathione and creatine kinase activity) and undergo assessments of their delay onset muscle soreness (DOMS), maximal voluntary isometric contraction (MVIC) of knee extensors and flexors (by isokinetic dynamometer), height and power of countermovement jump (CMJ-using force platform), 10m and 30m sprint time (will be evaluated using light cells). A 7-day wash-out period will apllied between ST and CT. The blood sample will be collected before and 24, 48 and 72 hours post-taining. Also, the evaluation of DOMS, MVIC, sprinting performance, power and jump height (by CMJ) will be evaluated before and 24, 48, 72 and 96 hours post training.

• Study Type: Interventional
• Enrollment (Estimated): 11
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Ioannis G Fatouros, Prof; Phone Number: +30 24310 47047; Email: ifatouros@.pe.uth.gr
• Study Contact Backup: Name: Athanasios Z Jamurtas, Prof; Phone Number: +30 24310 47054; Email: ajamurt@pe.uth.gr

Study Locations

• Greece
  • Tríkala, Greece, 42100
    • Recruiting
    • University o Thessaly, School of Physical Education and Sports Science
    • Principal Investigator: Athanasios S Poulios, PhD
    • Contact: Athanasios Z Jamurtas, Prof; Phone Number: +30 24310 47054; Email: ajamurt@pe.uth.gr
    • Contact: Ioannis G Fatouros, Prof; Phone Number: +30 24310 47047; Email: ifatouros@pe.uth.gr

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Free of musculoskeletal injuries
• No use of ergogenic supplements or medication
• Free of chronic diseases
• age of 40-60 years

Exclusion Criteria:

• Musculoskeletal injury
• Use of alcohol, caffeine and any type of ergogenic supplements or medication during the course of the study

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
No Intervention: Control; Participants in this arm will receive no intervention
Experimental: ST; Participants in this arm will participate in a soccer training (ST) session for 60 minutes. During the training the participans will execute the warm-up process, the part of soccer technical exercises and they will play a small-side-game.	Other: ST; Participants will perform a soccer training (ST) session for 60 minutes. The training will include the warm-up, soccer technical exercises and a small-side game

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in White blood cell count	White blood cell count will be measured using an automatic blood analyzer	At baseline, at 24, 48 and 72 hours after soccer training
Change in Granulocyte cell count	Change in White blood cell count will be measured using an automatic blood analyzer	At baseline, at 24, 48 and 72 hours after soccer training
Change in Platelets cell count	Platelets cell count will be measured using an automatic blood analyzer	At baseline, at 24, 48 and 72 hours after soccer training
Change in Monocyte cell count	Monocytes cell count will be measured using an automatic blood analyzer	At baseline, at 24, 48 and 72 hours after soccer training
Change in Lymphocyte cell count	Lymphocyte cell count will be measured using an automatic blood analyzer	At baseline, at 24, 48 and 72 hours after soccer training
Change in percentage of Hematocrit level	The percentage of Hematocrit level will be measured using an automatic blood analyzer	At baseline, at 24, 48 and 72 hours after soccer training
Change Red blood cells count	Red blood cells count will be measured using an automatic blood analyzer	At baseline, at 24, 48 and 72 hours after soccer training
Change in Hemoglobin level	Hemoglobin level will be measured using an automatic blood analyzer	At baseline, at 24, 48 and 72 hours after soccer training
Change in Creatine kinase activity	Creatine kinase activity will be measured in plasma	At baseline, at 24, 48 and 72 hours after soccer training
Change in Total antioxidant capacity	Total antioxidant capacity will be measured in plasma	At baseline, at 24, 48 and 72 hours after soccer training
Change in Glutathione concentration in blood	Glutathione concentration will be measured in red blood cells	At baseline, at 24, 48 and 72 hours after soccer training
Change in countermovement jump height	countermovement jump height will be measured using a force platform	At baseline, at 24, 48, 72 and 96 hours after soccer training
Change in Peak power during coutermovement jump test	The peak power will be measured using a force platform	At baseline, at 24, 48, 72 and 96 hours after soccer training
Change in isometric peak torque of knee extensors and flexors	Isometric peak torque will be assessed on an isokinetic dynamometer	At baseline, at 24, 48, 72 and 96 hours after soccer training
Change in sprint time of 10 m	10m sprint time will be assessed using light cells	At baseline, at 24, 48, 72 and 96 hours after soccer training
Change in sprint time of 30 m	30m sprint time will be assessed using light cells	At baseline, at 24, 48, 72 and 96 hours after soccer training
Change in delayed onset of muscle soreness	Muscle soreness will be assessed by palpation of muscle belly and the distal region	At baseline, at 24, 48, 72 and 96 hours after soccer training

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Lean body mass	Lean body mass will be measured by using Dual-emission X-ray absorptiometry	At baseline
Dietary intake	Dietary intake will be assesed over a 7-day period using diet recalls	At baseline
Peak Maximal oxygen consumption (Peak VO2)	Peak VO2 will be estimated by open circuit spirometry via breath by breath method	At baseline
Body Mass	Body Mass will be measured on a beam balance with stadiometer	At baseline
Body Height	Body Height will be measured on a beam balance with stadiometer	At baseline
Body Fat	Body Fat will be measured by using Dual-emission X-ray absorptiometry	At baseline
Bone mass density	Bone mass density will be measured by using Dual-emission X-ray absorptiometry	At baseline
Bone mass content	Bone mass content will be measured by using Dual-emission X-ray absorptiometry	At baseline
Field activity during the soccer training	The field activity will be continuously monitored during the soccer training using global positioning system (GPS)	During the 60 minutes soccer training (ST) session (72 hours after baseline measurements)
Heart rate during the soccer training	Heart rate will be continuously monitored during the soccer training using heart rate monitors	During the 60 minutes soccer training (ST) session (72 hours after baseline measurements)
Systolic Blood Pressure	Systolic Blood Pressure will be examined using sphygmomanometer	At baseline
Dystolic Blood Pressure	Dystolic Blood Pressure will be examined using sphygmomanometer	At baseline
Rest Heart Rate	The rest heart rate will be estimated using a heart rate monitor	At baseline

Collaborators and Investigators

• Sponsor: University of Thessaly
• Investigators: Study Director: Ioannis G Fatouros, Prof, University of Thessaly

Study record dates

Study Major Dates

• Study Start (Actual): February 5, 2024
• Primary Completion (Estimated): February 26, 2024
• Study Completion (Estimated): March 9, 2024

Study Registration Dates

• First Submitted: January 29, 2024
• First Submitted That Met QC Criteria: February 6, 2024
• First Posted (Actual): February 15, 2024

Study Record Updates

• Last Update Posted (Estimated): February 21, 2024
• Last Update Submitted That Met QC Criteria: February 19, 2024
• Last Verified: February 1, 2024

More Information

Terms related to this study

• Keywords: Recovery; Soccer; inflammation; performance; Soccer training
• Other Study ID Numbers: FFH

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No