The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains.

The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains in Elite Rowers After 2000m Ergometer Test - an Observational Study.

The study aimed to check the influence of timing and composition of diet on markers of gut injury, endotoxemia and stress.

Study Overview

• Status: Completed
• Conditions: Endothelial Dysfunction
• Intervention / Treatment: Other: 2000 meter ergometer test

Detailed Description

The research protocol includes detailed food intake analysis, including timing and composition, markers related to the neuroendocrine pathway cortisol, and observations of individually experienced symptoms. Moreover, the study also explores intestinal injury and endotoxemia parameters, such as I-FABP, CLDN-3, and LBP. As a first goal, this study investigates the timing and composition of food intake concerning changes in gut integrity, cortisol levels, and gastrointestinal symptoms. Investigators explored whether an athlete's nutritional intake influences any GI disturbance induced by high-intensity exercise. As a second goal, Investigators tried to connect symptoms and markers from both pathways with gastrointestinal complaints. Investigators hypothesised that cortisol levels would be more profound than other studied parameters in gastrointestinal symptoms.

• Study Type: Observational
• Enrollment (Actual): 13

Contacts and Locations

Study Locations

• Poland
  • Lubuskie
    • Gorzów Wielkopolski, Lubuskie, Poland, 66-400
      • Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

• Sampling Method: Non-Probability Sample

Study Population

Male rowers age 18 - 22 years.

Description

Inclusion Criteria:

• Membership in Polish Rowing Team,
• minimum five years of training,
• total training time minimum of 240 minutes,
• filling out a food diary,
• finishing 2000-meter ergometer test.

Exclusion Criteria:

• Probiotics within the last three months,
• prebiotics within the last three months
• antibiotic therapy within the last three months,
• dietary regime,
• gastrointestinal diseases,
• lactose intolerance

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury	I-FABP concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
Claudin to measure tight junction leakage	Zonulin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
LBP (lipopolysaccharide binding protein) to measure endotoxin	LBP concentration measured in blood [ug/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
Lactic acid to measure fatigue after the race	Lactate concentration measured in capillary blood from earlobe [mmol/L] using a commercially available kit (Diaglobal, Berlin, Germany)	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test
Corisol	Stress hormoneconcentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
energy	energy [kcal ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
protein	protein [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
fat	fat [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
carbohydrate	carbohydrate [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
glucose	glucose [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
lactose	lactose [g ]of food intake measured by food diary	whole day(24hours) before test, in the morning before the test
saccharose	saccharose [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
fructose	fructose [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
fiber	fiber [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
Body mass	Body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test up to 1 hour after getting up
Body fat	Body fat [%] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test up to 1 hour after getting up
Total body water	Body fat [%] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test up to 1 hour after getting up
Lean body mass	Lean body mass [kg] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test up to 1 hour after getting up
height	[cm] measured by high meter	in the morning before the test up to 1 hour after getting up
self reported gastrointestinal scale	10 point scale	up to 1 hours after the test

Collaborators and Investigators

• Sponsor: Poznan University of Physical Education
• Investigators: Study Director: Anna Skarpańska-Stejnborn, professor, Poznan University of Physical Education

Study record dates

Study Major Dates

• Study Start (Actual): May 1, 2023
• Primary Completion (Actual): June 1, 2023
• Study Completion (Actual): June 30, 2023

Study Registration Dates

• First Submitted: April 26, 2024
• First Submitted That Met QC Criteria: May 6, 2024
• First Posted (Actual): May 7, 2024

Study Record Updates

• Last Update Posted (Actual): May 7, 2024
• Last Update Submitted That Met QC Criteria: May 6, 2024
• Last Verified: June 1, 2023

More Information

Terms related to this study

• Keywords: exercise; gut permeability; cortisol; endotoxemia; athlete's diet; gut injury
• Other Study ID Numbers: Rowerscometetive2023

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No