Injectable PRF in Non-Surgical Periodontal Treatment (IPRF-PERIO)

January 28, 2026 updated by: Thuraya ayad taher al-jubori, Al-Mustansiriyah University

Adjunctive Use of Injectable PRF in Non-surgical Periodontal Therapy: Clinical and Microbiological Outcomes ( Split - Mouth Randomized Controlled Trial )

This study aims to evaluate the clinical and microbiological effects of using injectable Platelet-Rich Fibrin (i-PRF) as an adjunct to non-surgical periodontal therapy (scaling and root planing) in patients with periodontitis. The study uses a split-mouth design where one side of the mouth receives the treatment with i-PRF and the other side receives standard treatment alone. Clinical parameters and bacterial counts will be compared at baseline, 1 month, and 3 months after intervention .

Study Overview

Status

Active, not recruiting

Conditions

Intervention / Treatment

Detailed Description

Patients with periodontitis will be recruited for this split-mouth randomized controlled trial. All participants will receive full-mouth supra and sub-gingival scaling and root planing (SRP). In the intervention side, i-PRF will be injected into the periodontal pockets, while the control side will receive SRP only.

Clinical measurements including Probing Pocket Depth (PPD), Clinical Attachment Level (CAL), and Bleeding on Probing (BOP) will be recorded. Additionally, microbiological samples will be collected to assess the changes in the subgingival Porphyromonas gingivalis and Fusobacterium nucleatum . Follow-up visits are scheduled at 1 and 3 months post-intervention to assess the adjunctive benefit of i-PRF.

Study Type

Interventional

Enrollment (Actual)

13

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Iraq
      • Baghdad, Iraq
        • Mustansiriyah University, College of Dentistry.

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult
  • Older Adult

Accepts Healthy Volunteers

No

Description

Inclusion Criteria:

  • Adult subjects aged 30 years and above.
  • No history of systemic diseases (e.g., diabetes mellitus).
  • Not currently under active periodontal therapy or joining other trial in the last 3 months.
  • Diagnosis with Stage II or III Periodontitis.
  • presence of interdental CAL affecting ≥2 non adjacent teeth or if CAL at buccal/lingual aspects associated with PPD >4mm at ≥2 teeth.

Exclusion Criteria:

  • Smokers or alcohol users.
  • Patients consuming antibiotics or anticoagulants.
  • Regular users of non-steroidal anti-inflammatory drugs.
  • Receipt of periodontal treatment within 3 months prior to the study.
  • Any bleeding or clotting disorders.
  • Teeth with Grade II or Grade III furcation involvement.
  • Pregnant or breastfeeding mothers.
  • Patients unwilling to participate.

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Treatment
  • Allocation: Randomized
  • Interventional Model: Crossover Assignment
  • Masking: Double

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Experimental: Experimental: i-PRF + SRP
This quadrant of the mouth will receive full-mouth scaling and root planing (SRP) followed by the subgingival injection of injectable Platelet-Rich Fibrin (i-PRF) into the periodontal pockets
Biological: Injectable Platelet-Rich Fibrin (i-PRF).
Ten ml of whole blood is collected from the participant into plain tubes and immediately centrifuged. The centrifugation protocol follows the low-speed concept (e.g., 700 rpm for 3 minutes). The upper orange-colored liquid (i-PRF) is then collected and injected subgingivally into the periodontal pockets using a 25-gauge needle immediately after scaling and root planing
Other Names:
  • i-PRF
  • Autologous Platelet Concentrate.
Procedure: Scaling and Root Planing (SRP).
Full-mouth non-surgical periodontal therapy including supra and sub-gingival scaling and root planing performed using ultrasonic devices and Gracey curettes to remove dental biofilm and calculus
Other Names:
  • SRP
  • Non surgical periodontal therapy
  • Initial periodontal therapy
  • Phase I periodontal therapy
  • Cause related periodontal therapy
Active Comparator: Active Comparator: SRP Alone
This contralateral quadrant of the mouth will receive full-mouth scaling and root planing (SRP) only, serving as the control side to compare with the i-PRF intervention.
Procedure: Scaling and Root Planing (SRP).
Full-mouth non-surgical periodontal therapy including supra and sub-gingival scaling and root planing performed using ultrasonic devices and Gracey curettes to remove dental biofilm and calculus
Other Names:
  • SRP
  • Non surgical periodontal therapy
  • Initial periodontal therapy
  • Phase I periodontal therapy
  • Cause related periodontal therapy

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Change in Probing Pocket Depth (PPD)
Time Frame: Baseline, 1 month, and 3 months post-intervention
Probing pocket depth will be measured in millimeters from the gingival margin to the base of the periodontal pocket using a standardized periodontal probe (e.g., William's probe) at six sites per tooth.
Baseline, 1 month, and 3 months post-intervention

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Clinical Attachment Level (CAL)
Time Frame: Baseline, 1 month, and 3 months post-intervention
Measurement of the distance from the cemento-enamel junction (CEJ) to the base of the periodontal pocket in millimeters.
Baseline, 1 month, and 3 months post-intervention
Bleeding on Probing (BOP)
Time Frame: Baseline, 1 month, and 3 months post intervention
Assessment of the presence or absence of bleeding within 30 seconds after probing, recorded as a percentage of total sites.
Baseline, 1 month, and 3 months post intervention
Plaque Index (PI)
Time Frame: Baseline, 1 month, and 3 months post intervention .
Assessment of the amount of dental plaque on the tooth surfaces to evaluate the patient's oral hygiene during the study.
Baseline, 1 month, and 3 months post intervention .
Microbiological Analysis (Bacterial Load)
Time Frame: Baseline, 1 month, and 3 months post intervention
Quantification of subgingival periodontal pathogens (P. gingivalis and F. nucleatum )using Quantitative Real-time PCR (qPCR) from subgingival plaque samples.
Baseline, 1 month, and 3 months post intervention

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Al-Mustansiriyah University

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

November 6, 2025

Primary Completion (Estimated)

April 28, 2026

Study Completion (Estimated)

May 6, 2026

Study Registration Dates

First Submitted

January 21, 2026

First Submitted That Met QC Criteria

January 21, 2026

First Posted (Actual)

January 29, 2026

Study Record Updates

Last Update Posted (Actual)

January 30, 2026

Last Update Submitted That Met QC Criteria

January 28, 2026

Last Verified

January 1, 2026

More Information

Terms related to this study

Keywords

Additional Relevant MeSH Terms

Other Study ID Numbers

  • UOM-DENT-ETHIC-2026-042

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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