- ICH GCP
- US Clinical Trials Registry
- Klinisk forsøg NCT00162838
Effects of Periodontal Pathogens, Porphyromonas Gingivalis and Tannerella Forsythensis, on Cytokine Production From Human Monocyte-Derived Dendritic Cells
22. november 2005 opdateret af: National Taiwan University Hospital
Effects of Periodontal Pathogens, Porphyromonas Gingivalis and Tannerella Forsythensis, on Cytokine Production From Human Monocyte-Derived Dendritic Cells.
Periodontitis develops due to subgingival infection with specific microbial pathogen from dental plaque.
The bacteria can activate immunoinflammatory mechanisms within the local periodontal tissues that lead to destruction of collagen and alveolar bone.
Human gingiva contains Langerhans and connective tissue dendritic cells.
Signals from periodontal pathogen can induce dendritic cells to maturation,rapidly increasing surface expression of MHC class II, costimulatory molecules, and secrete proinflammatory cytokines to regulate adaptive T cell immune response.
Studies on cytokines have led to controversy about different T cell subsets associated with the progression of periodontitis.
Seymour proposed that susceptibility to periodontal disease progression involve a predominantly Th2 response while Ebersole speculated that Th2 cells providing protective function.
It is possible that a given pathogen may produce different maturation signals by activating DCs induce a given type of immune response.
In this study, we observed the profiles and amounts of cytokine production of DCs stimulated with P. gingivalis and T. forsythensis compared with E. coli, to see whether the periodontal pathogens may induce different response of dendritic cells in the innate immunity.
Studieoversigt
Status
Ukendt
Betingelser
Detaljeret beskrivelse
Periodontitis develops due to subgingival infection with specific microbial pathogen from dental plaque.
The bacteria can activate immunoinflammatory mechanisms within the local periodontal tissues that lead to destruction of collagen and alveolar bone.
Bacterial antigens can release from the supra and subgingival dental plaque on the tooth surface as well as from bacteria attached to mucosal surfaces.
The sulcular epithelium served as a physical barrier to the insult of the bacteria, the presence of Langerhans cells is also responsible for communication with the immune system.
Dendritic cells (DCs), the most effective antigen-presenting cells, contact and process the antigens.
Signals from pathogen induce dendritic cells to maturation.
Mature DCs rapidly increase surface expression and stability of MHC class I and class II-peptide complexes, upregulate the surface expression of adhesion and co-stimulatory molecules (CD40, CD54, CD80, and CD86) and secrete proinflammatory cytokines such as IL-1, IL-6, IL-12…….. then they migrate to lymphoid organs, where they regulate T cell immune response.
Human gingiva contains Langerhans and connective tissue dendritic cells.
Immature dendritic cells appeared to be limited to the epithelium, whereas mature dendritic cells were restricted to the connective tissue.
Studies on cytokines have led to controversy about the the different T cell subsets associated with the progression of periodontitis.
Seymour proposed that susceptibility to periodontal disease progression involve a predominantly Th2 response while Ebersole speculated that Th2 cells providing protective function.
Progression of periodontitis is also associated with the presence of gram-negative anaerobic microorganism such as Porphyromonas gingivalis, Bacteroides forsythus.
Different bacteria and their LPS can elicit strikingly different response.
It is possible that a given pathogen may produce different maturation signals by selectively activating a particular DC subset to induce a given type of immune response.
In this study, we observed the profiles and amounts of cytokine production of DCs stimulated with P. gingivalis and T. forsythensis compared with E. coli, to see whether the periodontal pathogens may induce different response of dendritic cells in the innate immunity.
Undersøgelsestype
Observationel
Tilmelding
20
Kontakter og lokationer
Dette afsnit indeholder kontaktoplysninger for dem, der udfører undersøgelsen, og oplysninger om, hvor denne undersøgelse udføres.
Studiesteder
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Taipei, Taiwan
- Rekruttering
- National Taiwan University Hospital
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Kontakt:
- Man ying Wong
- Telefonnummer: 7700 +886-2-23123456
- E-mail: veronica@ha.mc.ntu.edu.tw
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Ledende efterforsker:
- Man ying Wong
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Deltagelseskriterier
Forskere leder efter personer, der passer til en bestemt beskrivelse, kaldet berettigelseskriterier. Nogle eksempler på disse kriterier er en persons generelle helbredstilstand eller tidligere behandlinger.
Berettigelseskriterier
Aldre berettiget til at studere
20 år og ældre (Voksen, Ældre voksen)
Tager imod sunde frivillige
Ja
Køn, der er berettiget til at studere
Alle
Beskrivelse
Inclusion Criteria:
- Healthy
Exclusion Criteria:
-
Studieplan
Dette afsnit indeholder detaljer om studieplanen, herunder hvordan undersøgelsen er designet, og hvad undersøgelsen måler.
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
Samarbejdspartnere og efterforskere
Det er her, du vil finde personer og organisationer, der er involveret i denne undersøgelse.
Efterforskere
- Ledende efterforsker: Man-ying Wong, DDS, MS, Department of Periodontics, National Taiwan University Hospital
Datoer for undersøgelser
Disse datoer sporer fremskridtene for indsendelser af undersøgelsesrekord og resumeresultater til ClinicalTrials.gov. Studieregistreringer og rapporterede resultater gennemgås af National Library of Medicine (NLM) for at sikre, at de opfylder specifikke kvalitetskontrolstandarder, før de offentliggøres på den offentlige hjemmeside.
Studer store datoer
Studiestart
1. oktober 2003
Datoer for studieregistrering
Først indsendt
12. september 2005
Først indsendt, der opfyldte QC-kriterier
12. september 2005
Først opslået (Skøn)
13. september 2005
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Skøn)
23. november 2005
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
22. november 2005
Sidst verificeret
1. maj 2003
Mere information
Begreber relateret til denne undersøgelse
Yderligere relevante MeSH-vilkår
Andre undersøgelses-id-numre
- 9261700824
- NTUH-93S039
Disse oplysninger blev hentet direkte fra webstedet clinicaltrials.gov uden ændringer. Hvis du har nogen anmodninger om at ændre, fjerne eller opdatere dine undersøgelsesoplysninger, bedes du kontakte register@clinicaltrials.gov. Så snart en ændring er implementeret på clinicaltrials.gov, vil denne også blive opdateret automatisk på vores hjemmeside .
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