Mucin Concentration in Sputum From COPD Patients During a Pulmonary Exacerbation (mucinCOPDex)
Molekularbiologische Eigenschaften Des Sputums während Einer Pulmonalen COPD Exacerbation
chronic obstructive pulmonary disease (COPD) is typically associated with mucus hypersecretion in the airways. In health, mucin is the major macromolecular component and is responsible for the protective and clearance properties of the mucus gel. In a recent study the investigators found that mucins are decreased and unstable in the sputum of adult cystic fibrosis (CF) patients.
In this study the investigators want to investigate the differences on the mucin quantity and quality of airway secretions during pulmonary exacerbation of patients with COPD.
研究概览
详细说明
We hypothesize that during an exacerbation the mucin amount is increasing.
The aim of this study is to evaluate the molecular (mucins) and structure properties (mucin-stability) of the airway secretions in COPD related to the severity of the disease.
We characterize sputum composition of patients with pulmonary exacerbations. Using gel electrophoresis, with specific antibodies we will analyze MUC5AC and MUC5B mucins.
The significance of these studies is that they will give us novel information about the pathogenesis of chronic inflammatory airway diseases, provide tools for assessing the progression of lung disease, and most critically, will identify novel opportunities and targets for therapeutic intervention.
研究类型
联系人和位置
学习地点
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Marburg、德国、35043
- pulmonary department, University Marburg
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Marburg、德国
- University Marburg
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
描述
Inclusion Criteria:
- forced expiratory volume at one second (FEV1) < 80%
- sputum production
- clinical likely hood of exacerbation
Exclusion Criteria:
- FEV1 > 80% or < 30%
- increased systemic inflammation
- susceptibility of pneumonia
- need for antibiotic treatment
学习计划
研究是如何设计的？
设计细节
- 观测模型：队列
- 时间观点：追溯
队列和干预
团体/队列 |
干预/治疗 |
|---|---|
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COPD exacerbation
COPD Stadium 2 and 3 during pulmonary exacerbation sputum collection |
collecting of spontaneous sputum from the patient
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研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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mucin concentration
大体时间：2 month
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analyzing mucin concentration by western
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2 month
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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mucin stability
大体时间：2 month
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analyzing mucin stability at 37C over 24 hours
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2 month
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合作者和调查者
调查人员
- 首席研究员：Markus Henke, MD、University Marburg
出版物和有用的链接
一般刊物
- Henke MO, Renner A, Huber RM, Seeds MC, Rubin BK. MUC5AC and MUC5B Mucins Are Decreased in Cystic Fibrosis Airway Secretions. Am J Respir Cell Mol Biol. 2004 Jul;31(1):86-91. doi: 10.1165/rcmb.2003-0345OC. Epub 2004 Feb 26.
- Henke MO, John G, Rheineck C, Chillappagari S, Naehrlich L, Rubin BK. Serine proteases degrade airway mucins in cystic fibrosis. Infect Immun. 2011 Aug;79(8):3438-44. doi: 10.1128/IAI.01252-10. Epub 2011 Jun 6.
- Henke MO, John G, Germann M, Lindemann H, Rubin BK. MUC5AC and MUC5B mucins increase in cystic fibrosis airway secretions during pulmonary exacerbation. Am J Respir Crit Care Med. 2007 Apr 15;175(8):816-21. doi: 10.1164/rccm.200607-1011OC. Epub 2007 Jan 25.
研究记录日期
研究主要日期
学习开始
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (估计)
研究记录更新
最后更新发布 (估计)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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慢性阻塞性肺病的临床试验
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Bangabandhu Sheikh Mujib Medical University, Dhaka...完全的
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Virginia Commonwealth UniversityFisher and Paykel Healthcare完全的
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University College, LondonUniversity of Cambridge; National Institute for Health Research, United Kingdom; Royal Free Hampstead...未知
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AstraZeneca完全的
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Chiesi Farmaceutici S.p.A.完全的中度至重度慢性阻塞性肺疾病 (COPD)保加利亚, 德国, 匈牙利, 波兰, 俄罗斯联邦, 英国
sputum collection的临床试验
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Cliniques universitaires Saint-Luc- Université...Université de Liège招聘中
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