The Implementation of Novel 'Heavy' Water Techniques for Determining Modulation of Muscle Protein, DNA and Lipid Synthesis Due to Ageing

Maintenance of body's skeletal muscle is key to a healthy older age. However, as we age we lose on average 1-2% of our muscle each year. In order to slow this loss we need to understand fully the mechanisms regulating muscle mass with ageing. In this project we aim to determine these mechanisms using a new novel technique of 'heavy' water ingestion, which will allow us to measure multiple aspects of skeletal muscle mass control during normal everyday activities in young and old individuals over a period of 6 weeks. We will also determine the influence of resistance exercise training during this period in offsetting declines in muscle with ageing using this method

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Behavioral: Resistance Exercise

• Study Type: Interventional
• Enrollment (Actual): 20
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United Kingdom
  • Derby, United Kingdom, DE22 3DT
    • Royal Derby Hospital Medical School

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 75 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Young (18-30y) and old men (65-75y) who are generally healthy and recreationally active

Exclusion Criteria:

• Active cardiovascular disease
• Cerebrovascular disease including previous stroke, aneurysm (large vessel or intracranial)
• Respiratory disease including pulmonary hypertension or COPD
• Hyper/ hypo parathyroidism, hyper/ hypothyroidism, Cushing's disease, diabetes
• Active inflammatory bowel disease
• Renal disease
• Malignancy
• Recent steroid treatment (within 6 mo), or hormone replacement therapy
• Clotting dysfunction
• Musculoskeletal or neurological disorders
• Any disease requiring long-term drug prescriptions, including statins

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Old Unilateral Exercise; Old individuals (65-75y) studied before and after 6 weeks of unilateral resistance exercise training	Behavioral: Resistance Exercise; 6 Weeks progressive unilateral resistance exercise, 3 times per week, 70% 1-RM, 6 x 8 Repetitions. 2 minutes rest between sets
Experimental: Young Unilateral Exercise; Young Individuals (18-30y) studied before and after 6 weeks unilateral resistance exercise training	Behavioral: Resistance Exercise; 6 Weeks progressive unilateral resistance exercise, 3 times per week, 70% 1-RM, 6 x 8 Repetitions. 2 minutes rest between sets

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Muscle Protein Synthesis	Comparison of muscle protein synthesis between young and older individuals and in response to 6 weeks unilateral exercise training	0-6 Weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Muscle Sub fractions	Comparison of different sub fractions involved in muscle mass regulation between young and older individuals and in response unilateral exercise training	0-6 week

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Muscle Functions	Muscle Strength and power un young and older individuals in response to unilateral exercise training	0-6 Weeks

Collaborators and Investigators

• Sponsor: University of Nottingham
• Collaborators: Physiological Society
• Investigators: Principal Investigator: Philip Atherton, PhD, AFHEA, University of Nottingham

Study record dates

Study Major Dates

• Study Start: May 1, 2013
• Primary Completion (Actual): July 1, 2014
• Study Completion: December 7, 2022

Study Registration Dates

• First Submitted: May 29, 2014
• First Submitted That Met QC Criteria: May 30, 2014
• First Posted (Estimate): June 2, 2014

Study Record Updates

• Last Update Posted (Estimate): July 31, 2015
• Last Update Submitted That Met QC Criteria: July 30, 2015
• Last Verified: July 1, 2015

More Information

Terms related to this study

• Other Study ID Numbers: Physoc_D20

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No