Serum Lipid Levels and Lp-PLA2 in Chronic Periodontitis

The Association Between Periodontal Status, Serum Lipid Levels and Lipoprotein Associated Lipophosphase A2(Lp-PLA2) in Chronic Periodontitis Subjects and Healthy Controls: A Case Control Study

To assess the association between periodontal parameters, serum lipid profile and Lp-PLA2 level in systemically healthy Chronic Periodontitis subjects and healthy controls.

Study Overview

• Status: Completed
• Conditions: Chronic Periodontitis (Disorder)
• Intervention / Treatment: Diagnostic test: serum lipid levels and Lp-PLA2

Detailed Description

Seventy five participants were divided into 3 groups. The groups consisted of GROUP I- generalized severe chronic periodontitis, GROUP II- generalized moderate chronic periodontitis and GROUP III- systemically and periodontally healthy volunteers who served as control. The selected patients were then evaluated for periodontal parameters, lipid profile and Lp-PLA2.

• Study Type: Observational
• Enrollment (Actual): 75

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 33 years to 62 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

The study population was selected from the Department of Periodontology, Meenakshi Ammal Dental College

Description

Inclusion Criteria:

Group I:

1. Subjects with age ≥ 35 years.
2. Presence of at least 24 teeth, with Clinical attachment loss (CAL) ≥ 5mm in more than 30% of sites.

Group II:

1. Subjects with age ≥ 35 years.
2. Presence of at least 24 teeth, with Clinical attachment loss (CAL) between 3-4 mm in more than 30% of sites.

Group III:

1. Subjects with age ≥ 35 years.
2. Healthy and intact periodontium

Exclusion Criteria:

1. Presence of any systemic disease or conditions that could affect periodontal tissues.
2. History of periodontal treatment in past 6 months.
3. Smoking and alcoholism
4. Anomalies of blood and immune system
5. Systemic medication or antibiotic treatment for the previous 6 months.
6. Pregnant females.

Study Plan

How is the study designed?

Design Details

• Observational Models: Case-Control
• Time Perspectives: Other

Number of groups / cohorts

3

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
GROUP I; Estimation of serum lipid levels and Lp-PLA2 in generalized severe chronic periodontitis	Diagnostic test: serum lipid levels and Lp-PLA2; Estimation of Total Cholesterol, triglyceride, High density lipoprotein, Low density lipoprotein, very low density lipoprotein and Lipoprotein associated lipophosphase A2 in chronic periodontitis; Other Names: Biological marker
GROUP II; Estimation of serum lipid levels and Lp-PLA2 in generalized moderate chronic periodontitis	Diagnostic test: serum lipid levels and Lp-PLA2; Estimation of Total Cholesterol, triglyceride, High density lipoprotein, Low density lipoprotein, very low density lipoprotein and Lipoprotein associated lipophosphase A2 in chronic periodontitis; Other Names: Biological marker
GROUP III; Estimation of serum lipid levels and Lp-PLA2 in systemically and periodontally healthy controls	Diagnostic test: serum lipid levels and Lp-PLA2; Estimation of Total Cholesterol, triglyceride, High density lipoprotein, Low density lipoprotein, very low density lipoprotein and Lipoprotein associated lipophosphase A2 in chronic periodontitis; Other Names: Biological marker

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Estimation of serum Lp-PLA2	Serum Lp-PLA2 was estimated in all the groups using ELISA	9 months

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Estimation of serum lipid profile	Serum Total cholesterol, triglyceride, High density lipoprotein, Low density lipoprotein and very low density lipoprotein	9 months

Collaborators and Investigators

• Sponsor: Meenakshi Ammal Dental College and Hospital
• Investigators: Principal Investigator: Bittu Koshy, BDS, Meenakshi Ammal Dental College

Publications and helpful links

General Publications

• Zhou SY, Xiao WM, Ouyang XY. Lipoprotein-associated phospholipase A2 and serum lipid levels in subjects with chronic periodontitis and hyperlipidemia. Chin J Dent Res. 2012;15(1):25-9.
• Fentoglu O, Koroglu BK, Kara Y, Dogan B, Yilmaz G, Sutcu R, Ay ZY, Tonguc MO, Orhan H, Tamer MN, Kirzioglu FY. Serum lipoprotein-associated phospholipase A(2) and C-reactive protein levels in association with periodontal disease and hyperlipidemia. J Periodontol. 2011 Mar;82(3):350-9. doi: 10.1902/jop.2010.100417. Epub 2010 Sep 1.

Study record dates

Study Major Dates

• Study Start (Actual): February 2, 2015
• Primary Completion (Actual): August 23, 2015
• Study Completion (Actual): November 26, 2015

Study Registration Dates

• First Submitted: February 1, 2017
• First Submitted That Met QC Criteria: February 1, 2017
• First Posted (Estimate): February 2, 2017

Study Record Updates

• Last Update Posted (Estimate): February 2, 2017
• Last Update Submitted That Met QC Criteria: February 1, 2017
• Last Verified: January 1, 2017

More Information

Terms related to this study

• Keywords: lipids; chronic periodontitis; Lp-PLA2
• Additional Relevant MeSH Terms: Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Periodontitis; Chronic Periodontitis
• Other Study ID Numbers: MADC/IRB/2015/91

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No