Examining The Effect of Blood Flow Restricted Aerobic Training on Mitochondrial Adaptations in Young Healthy Adults

Examining The Effect of Blood Flow Restricted Aerobic Training on Citrate Synthase Activity in Young Healthy Adults

This parallel-arm trial study investigates the effects of restricting arterial blood flow during cycling exercise on citrate synthase. Participants will be randomized to either a blood flow restricted or non-blood flow restricted exercise training group. Both groups will complete seven, 30-minute exercise sessions over a 14-day period.

Study Overview

• Status: Completed
• Conditions: Mitochondrial Biogenesis
• Intervention / Treatment: Other: BFR Exercise Training; Other: CTL Exercise Training

• Study Type: Interventional
• Enrollment (Actual): 19
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Canada
  • Ontario
    • Kingston, Ontario, Canada, K7L3N6
      • Queen's University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• 3 hours of physical activity per week
• No previous cycling training
• No concurrent involvement in another training program
• Body mass index < 30 kg/m2

Exclusion Criteria:

• Presence of cardiovascular disease
• Presence of metabolic disease
• Taking regular oral medication
• Current smoker

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: BFR	Other: BFR Exercise Training; Participants will complete exercise training on a recumbent bike placed on a 45 degree incline (BFR).
Experimental: CTL	Other: CTL Exercise Training; Participants will complete exercise training on a recumbent bike placed on flat ground (CTL).

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Citrate Synthase	Fold change in citrate synthase maximal activity	3 days prior to start of exercise training and 3 days after completing exercise training

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Transcription factor A, mitochondrial (TFAM)	Fold change in TFAM protein	3 days before training to 3 days after training
Nuclear respiratory factor, 1 (NRF1)	Fold change in NRF-1 protein	3 days before training to 3 days after training
Maximal oxygen consumption (VO2peak)	Change in VO2peak (POST-PRE)	3 days before training to 3 days after training
Heart rate (HR)	Average HR	Every 5 minutes during each of the 7, 30-minute exercise sessions
Revolutions per minute (RPM)	Average RPM	Every 5 minutes during each of the 7, 30-minute exercise sessions
Blood [lactate]	Average blood lactate concentration	During all exercise sessions at 10 and 30 minutes
Rating of perceived exertion (RPE)	Average RPE	Every 5 minutes during each of the 7, 30-minute exercise sessions
Nuclear Factor, Erythroid 2 Like 2 (NFE2L2)	Fold change in NFE2L2	3 days before training to 3 days after training
Cytochrome c oxidase (COX)	Fold change in COX	3 days before training to 3 days after training
Electromyography (EMG)	Median frequency and average amplitude of EMG signal	Throughout each of the 7, 30-minute exercise sessions
Near-infrared spectroscopy (NIRS)	Deoxygenated hemoglobin and oxygenated hemoglobin	Throughout each of the 7, 30-minute exercise sessions

Collaborators and Investigators

• Sponsor: Brendon Gurd, PhD
• Investigators: Principal Investigator: Brendon J Gurd, PhD, Queen's University

Study record dates

Study Major Dates

• Study Start (Actual): May 6, 2019
• Primary Completion (Actual): December 13, 2019
• Study Completion (Actual): December 13, 2019

Study Registration Dates

• First Submitted: April 2, 2019
• First Submitted That Met QC Criteria: November 1, 2019
• First Posted (Actual): November 5, 2019

Study Record Updates

• Last Update Posted (Actual): December 26, 2023
• Last Update Submitted That Met QC Criteria: December 19, 2023
• Last Verified: December 1, 2023

More Information

Terms related to this study

• Keywords: Aerobic exercise; Exercise training; Mitochondria; VO2; Blood flow restriction; Citrate Synthase
• Other Study ID Numbers: BFRT

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: UNDECIDED

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No