Incretin and Treatment With Inhibition of Sodium-glucose Cotransporter-2 Combination Insights Into Mechanisms Implicated in Congestive Heart Failure: "NATRIURETIC" Trial (NATRIURETIC)

This study aims to provide essential mechanistic insights into natriuretic and hemodynamic effects of SGLT2i and GLP-1RA agents in T2D patients. Ultimately, by obtaining physiological data in T2D patients without HF, our aims are to gain insight into how the use of this combined therapy may be used in T2D with HF in future work.

Study Overview

• Status: Recruiting
• Conditions: Type 2 Diabetes Mellitus
• Intervention / Treatment: Drug: Empagliflozin 25 MG + Liraglutide 1.8 MG; Drug: Liraglutide 1.8 MG + Empagliflozin 25 MG

Detailed Description

Type 2 diabetes (T2D) is an epidemic that afflicts more than 350 million people world-wide. Despite the use of existing medical therapies, T2D continues to cause significant morbidity and mortality, leading to large societal and financial costs to Canadians.

Newer agents called sodium glucose co-transporter-2 inhibitors (SGLT2i) have been developed to improve glycemic control and lower hemoglobin A1c by increasing glycosuria. SGLT2i also reduce blood pressure and albuminuria in T2D - possibly through natriuresis. Importantly, a landmark trial "EMPA-REG OUTCOME" demonstrated that the SGLT2i empagliflozin is the first anti- hyperglycemic agent to reduce mortality and heart failure (HF) risk, and also to decrease the risk of progressive diabetic nephropathy.

Liraglutide, a glucagon-like peptide-1 receptor agonist (GLP-1RA), is a subcutaneous drug, used for treating T2D patients. Recently, this drug was also able to reduce cardiovascular endpoints in the LEADER trial. Furthermore, liraglutide attenuated the risk of progressive albuminuria as well. GLP-1RAs induce natriuretic effects, but have not been shown to reduce HF risk. Their positive cardiovascular effects are probably due to reduction in atherosclerotic events.

T2D patients are at high risk for development of both HF and atherosclerotic diseases. Given that SGLT2i and GLP-1RA seem to reduce cardiovascular outcomes via different approaches, combining these two agents in the treatment of T2D is an appealing new strategy. In this project, we aim to combine GLP-1RA and SGLT2i therapies in T2D patients without HF and compare renal and cardiovascular physiological measures in these patients, such as renal function, neurohormonal activation, blood pressure, arterial stiffness, and systemic vascular resistance. This could give a better understanding on the action of the combination therapy in T2D, support future mechanist trials with patients with HF, and give support to the development of large clinical trials on this topic.

Study design: Open-label, exploratory, randomized (with randomization concealment), pilot mechanistic trial, with two groups and 2 sequential treatments within each group.

Study population: Male and female subjects with T2D, 18 years old or older, with eGFR ≥30 mL/min/1.73m2, as described in the full protocol.

Intervention: Patients will be randomized to initial therapy with empagliflozin 25mg PO daily or liraglutide 1.8 mg SC daily, for 6 weeks. After that, patients in the empagliflozin group will also receive liraglutide 1.8mg SC daily for additional 6 weeks and patients in the liraglutide group will also receive empagliflozin 25mg PO daily for 6 weeks (combination therapy).

Primary Objective: Comparison of proximal tubular natriuresis (measured by FENa+, fractional excretion of sodium) after 6 weeks of SGLT2i monotherapy vs FENa+ after 6 weeks of combination therapy (SGLT2i + GLP-1RA) AND comparison of FENa+ after 6 weeks of GLP1-RA monotherapy vs FENa+ after 6 weeks of combination therapy (GLP-1RA + SGLT2i).

Nature and extent of the burden and risks associated with participation, benefit and group relatedness: Patients visit the outpatient clinic on a more regular basis than standard patient care - i.e. at study inclusion and at each study visit for clinical assessment. Blood work for physiological assessments, renal function tests and cardiovascular assessments will be obtained as described in the protocol. 24hr urine will be collected one day prior to the hospital visit. No other invasive measurements will be executed. Patients receive restitution of all travel costs and also a financial aid for 3 visits. Patients receive no priority in treatment of other diseases in the clinic during this study. There are no other direct benefits for the patients to be included and participation is on a voluntary basis.

• Study Type: Interventional
• Enrollment (Anticipated): 36
• Phase: Phase 2

Contacts and Locations

• Study Contact: Name: Vesta Lai, RN; Phone Number: 8508 416-340-4800; Email: vesta.lai@uhn.ca
• Study Contact Backup: Name: Yuliya Lytvyn, PhD; Email: Julia.lytvyn@mail.utoronto.ca

Study Locations

• Canada
  • Ontario
    • Toronto, Ontario, Canada
      • Recruiting
      • Renal Physiology Laboratory
      • Principal Investigator: David Cherney, MD, PhD
      • Contact: Vesta Lai, RN; Phone Number: 8508 416-340-4800; Email: vesta.lai@uhn.ca
      • Contact: Yuliya Lytvyn, PhD; Email: Julia.lytvyn@mail.utoronto.ca

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

1. Men and women diagnosed with T2D ≥6 months prior to informed consent;
2. eGFR ≥30 mL/min/1.73m2;
3. Age >18 years;
4. HbA1c 7.0%-12.0%;
5. Body Mass Index (BMI) 18.5-40.0 kg/m2;
6. Stable HbA1c, measured 2-12 months prior to screening, within 5% of baseline value;
7. Blood pressure ≤160/100 mmHg at screening, >90/60 mmHg;
8. Stable on dose of ACE inhibitor, angiotensin receptor blocker or renin inhibitor for at least 2 weeks.

Exclusion Criteria:

1. Type 1 Diabetes;
2. Leukocyte and/or nitrite positive urinalysis that is untreated;
3. Severe hypoglycaemia within 2 months prior to screening;
4. History of hypoglycaemia unawareness based on investigator judgement;
5. Unstable coronary artery disease with acute coronary syndrome, percutaneous intervention or bypass surgery within 3 months;
6. Clinically significant valvular disease subject to PI/Sub PI's discretion;
7. Congestive heart failure subject to PI/Sub PI's discretion;
8. Bariatric surgery or other surgeries that induce chronic malabsorption within one year;
9. Anti-obesity drugs or diet regimen and unstable body weight three months prior to screening;
10. Treatment with systemic corticosteroids;
11. Blood dyscrasias or any disorders causing hemolysis or unstable red blood cells;
12. Pre-menopausal women who are nursing, pregnant, or of child-bearing potential and not practicing an acceptable method of birth control;
13. Participation in another trial with an investigational drug within 30 days of informed consent;
14. Alcohol or drug abuse within three months prior to informed consent that would interfere with trial participation or any ongoing clinical condition that would jeopardize subject safety or study compliance based on investigator judgement;
15. Liver disease, defined by serum levels of alanine transaminase, aspartate transaminase, or alkaline phosphatase >3 x upper limit of normal as determined during screening;
16. Medical history of cancer or treatment for cancer in the last five years prior to screening;
17. History of pancreatitis;
18. Personal of family history of medullary thyroid cancer or MEN2B;
19. Tachycardia, HR >100;
20. Use of SGLT2i, GLP-1RA or DPP-4i within the past 1 month (1-month minimum washout is allowed);
21. History of gastroparesis;
22. Known intolerance to SGLT2i or GLP-1RA;
23. Allergy to iodine-based substances if receiving iohexol for GFR measures.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Sequential Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Liraglutide; Liraglutide Subcutaneous Total Dose 1.8mg daily for 6 weeks	Drug: Empagliflozin 25 MG + Liraglutide 1.8 MG; Patients will be randomized to initial therapy with empagliflozin 25mg PO daily for 6 weeks. After that, patients in the empagliflozin group will also receive liraglutide 1.8mg SC daily for additional 6 weeks.; Other Names: Generic names are above, listed; Drug: Liraglutide 1.8 MG + Empagliflozin 25 MG; Patients will be randomized to initial therapy with liraglutide 1.8 mg SC daily, for 6 weeks. After that, patients will also receive empagliflozin 25mg PO daily for 6 weeks.; Other Names: Generic names are above, listed
Experimental: Empagliflozin; Empagliflozin Tablets Total Dose 25mg daily for 6 weeks	Drug: Empagliflozin 25 MG + Liraglutide 1.8 MG; Patients will be randomized to initial therapy with empagliflozin 25mg PO daily for 6 weeks. After that, patients in the empagliflozin group will also receive liraglutide 1.8mg SC daily for additional 6 weeks.; Other Names: Generic names are above, listed; Drug: Liraglutide 1.8 MG + Empagliflozin 25 MG; Patients will be randomized to initial therapy with liraglutide 1.8 mg SC daily, for 6 weeks. After that, patients will also receive empagliflozin 25mg PO daily for 6 weeks.; Other Names: Generic names are above, listed

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Proximal tubular natriuresis	Measured by fractional excretion of sodium	up to 12 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Glomerular Filtration Rate	GFR	Glomerular Filtration Rate (GFR, based on plasma iohexol clearance) will be measured at 2 time points: monotherapy (6 weeks) and combination therapy (12 weeks)
Systolic blood pressure	SBP	Systolic blood pressure (SBP) will be measured at 2 time points: monotherapy (6 weeks) and combination therapy (12 weeks)
Body weight	Kilograms	Body weight will be measured at 2 time points: monotherapy (6 weeks) and combination therapy (12 weeks)
Arterial stiffness	Measured using a Sphygmocor device	Arterial stiffness will be measured at 2 time points: monotherapy (6 weeks) and combination therapy (12 weeks)
Systemic vascular resistance	Measured using non-invasive cardiac output monitor (NICOM)	Systemic vascular resistance will be measured at 2 time points: monotherapy (6 weeks) and combination therapy (12 weeks)
Urinary concentration of the renin-angiotensin aldosterone system (RAAS) markers	Measured using ELISA	Outcome will be measured at 2 time points: monotherapy (6 weeks) and combination therapy (12 weeks)
Urinary EGF,FGF2,Eotaxin,TGFa,G-CSF,Flt-3L,GM-CSF,Fractalkine,IFNa2,IFNy,GRO,IL10,MCP-3,IL-12P40,MDC,IL-12P70,PDGF-AA,IL-13,PDGF-BB,IL-15,sCD40L,IL-17A,IL-1RA,IL1a,IL9,IL1B,IL2,IL3,IL4,IL5,IL6,IL7,IL8,IL10,MCP-1,MIP-1a,MIP-1B,RANTES,TNFalpha,TNFB,VEGF	41-plex chemo/chemokine profile assay: urinary	Outcome will be measured at 2 time points: monotherapy (6 weeks) and combination therapy (12 weeks)

Collaborators and Investigators

• Sponsor: University Health Network, Toronto
• Investigators: Principal Investigator: David ZI Cherney, MD PhD FRCPC, University Health Network, Toronto General Hospital

Study record dates

Study Major Dates

• Study Start (Actual): January 24, 2019
• Primary Completion (Anticipated): October 1, 2023
• Study Completion (Anticipated): October 1, 2023

Study Registration Dates

• First Submitted: February 12, 2020
• First Submitted That Met QC Criteria: September 1, 2020
• First Posted (Actual): September 2, 2020

Study Record Updates

• Last Update Posted (Actual): May 17, 2023
• Last Update Submitted That Met QC Criteria: May 15, 2023
• Last Verified: May 1, 2023

More Information

Terms related to this study

• Keywords: type 2 diabetes mellitus; SGLT2 inhibition; GLP-1 receptor agonist
• Additional Relevant MeSH Terms: Heart Diseases; Cardiovascular Diseases; Glucose Metabolism Disorders; Metabolic Diseases; Endocrine System Diseases; Heart Failure; Diabetes Mellitus; Diabetes Mellitus, Type 2; Hypoglycemic Agents; Physiological Effects of Drugs; Molecular Mechanisms of Pharmacological Action; Hormones; Hormones, Hormone Substitutes, and Hormone Antagonists; Incretins; Sodium-Glucose Transporter 2 Inhibitors; Liraglutide; Empagliflozin
• Other Study ID Numbers: 18-5058

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No