Effect of Beetroot Juice Supplements on Lower-limb Strength in Females

Acute Effects of Beetroot Juice Supplements on Lower-limb Strength and Muscular Endurance in Physically Active Women: Double-blind Crossover Randomized Trial

This study aims to evaluate the effects of beetroot juice supplements on lower-limb strength and muscular endurance in physically active women, due to the presence of NO3- in beetroot juice.

Study Overview

• Status: Completed
• Conditions: Muscular Endurance
• Intervention / Treatment: Dietary supplement: Beetroot juice; Other: Capri-Sun

Detailed Description

Beetroot juice (BJR) is used as an ergogenic aid, but no study has analyzed its' effects on explosive force production in physically active women. It has been proposed that females could blunt reduction of NO2- to NO based on a higher oxidative skeletal muscle phenotype than males. However, it has been observed a higher increase on plasma NO2- levels after supplementation compared to and a greater enhancement on muscle contractile function.

Based on the absence of any study that has analyzed the effect of NO3- supplements on jump ability and power production on weightlifting on female population; the purpose of this study will be to investigate the possible ergogenic effect of BJR on physically active women on explosive force production and muscular endurance in a lower-limb body weightlifting protocol, due to the presence of NO3- in BJR and its effects.

• Study Type: Interventional
• Enrollment (Actual): 11
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Brazil
  • Lavras, Brazil, 37200-000
    • Studies Research Group in Neuromuscular Responses (GEPREN), University of Lavras
• Spain
  • Córdoba, Spain, 14004
    • Maimonides Biomedical Research Institute of Cordoba (IMIBIC), Reina Sofia University Hospital, University of Cordoba
  • Córdoba, Spain, 14004
    • Metabolism and Investigation Unit, Maimonides Biomedical Research Institute of Cordoba (IMIBIC),Reina Sofia University Hospital, University of Cordoba
  • Córdoba, Spain, 14071
    • Department of Food Science and Technology, Rabanales University Campus, University of Cordoba
  • Córdoba, Spain, 14071
    • Department of Nursing Pharmacology and Physiotherapy, Faculty of Medicine and Nursing, University of Córdoba
  • Sevilla, Spain, 41013
    • Departamento de Motricidad Humana y Rendimiento Deportivo, Universidad de Sevilla

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Female

Description

Inclusion Criteria:

• Physically active females with 18-30 years of age
• More than 6 months on RT training programs
• Be familiarized with back squat, leg press and leg extension exercises
• Not consuming any type of nutritional supplement or anabolic substances in the previous three months or during the study
• Not suffering from a musculoskeletal injury that could interfere with the exercise protocol during the investigation

Exclusion Criteria:

• None

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: BJR; 70 mL of beetroot juice (BEET It Sport®; James White Drinks Ltd., Ipswich, UK)	Dietary supplement: Beetroot juice; Subjects will drink juice 120 minutes before each visit
Placebo Comparator: PLA; 70 mL of blackcurrant beverage Capri-Sun.	Other: Capri-Sun; 70 mL of blackcurrant beverage, which is depleted of NO3- will be taken 120 min before each visit.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change of height reached in jump	Evaluated through CMJ (Counter movement jump) test with a recovery period of 45 seconds between jumps.	Week 1, 2 and 3.

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Movement velocity	Evaluated at 50% and 75% repetition maximum (RM)	Visit 1 (week 1), 2 (week 2) and 3 (week 3).
Power on back squat	Evaluated at 50% and 75% repetition maximum (RM)	Visit 1 (week 1), 2 (week 2) and 3 (week 3).
Muscular endurance test	The test consists of three exercises: back squat, leg press and leg extension. Three series will be performed until concentric failure at 75% RM, three minutes of rest between each series.	Visit 1 (week 1), 2 (week 2) and 3 (week 3).
Rating of perceived exertion (RPE).	Immediately at the end of the muscular endurance test, participants will be instructed to report their RPE using a scale 1-10 (worse to better outcome).	Visit 1 (week 1), 2 (week 2) and 3 (week 3).

Collaborators and Investigators

• Sponsor: Maimónides Biomedical Research Institute of Córdoba
• Investigators: Principal Investigator: Jose Manuel Jurado Castro, Hospital Universitario Reina Sofia

Study record dates

Study Major Dates

• Study Start (Actual): July 5, 2021
• Primary Completion (Actual): August 29, 2021
• Study Completion (Actual): August 29, 2021

Study Registration Dates

• First Submitted: July 20, 2021
• First Submitted That Met QC Criteria: August 2, 2021
• First Posted (Actual): August 3, 2021

Study Record Updates

• Last Update Posted (Actual): August 31, 2021
• Last Update Submitted That Met QC Criteria: August 30, 2021
• Last Verified: July 1, 2021

More Information

Terms related to this study

• Keywords: Beetroot; Beetroot juice; muscular endurance; Explosive force production
• Other Study ID Numbers: BEETROOT JUICE

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: YES
• IPD Plan Description: Data will be shared after the study concludes and results are published
• IPD Sharing Time Frame: After results are published
• IPD Sharing Access Criteria: Upon request to study's principal investigator

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No