Breaking Sitting with High-intensity Interval Training for Brain Health (HIIT2SITLess)

Breaking Prolonged Sitting with High-intensity Interval Training to Improve Cognitive and Brain Health in Older Adults: a Pilot Feasibility Trial.

This trial will examine whether interrupting 3.5 hours of sitting every 30 min with 6 min high intensity interval training (HIIT) breaks compared to light intensity interval training (LIIT) will improve brain health in cognitively normal older adults. This trial will test the feasibility of HIIT breaks to sitting. It will also address several important but unanswered questions: (1) Does interrupting sitting with short HIIT breaks improve frontoparietal function? (2) Can interrupting sitting with HIIT breaks improve cognitive functions?

Study Overview

• Status: Recruiting
• Conditions: Aging
• Intervention / Treatment: Behavioral: High-Intensity Interval Training (HIIT) Breaks to Sitting; Behavioral: Light-Intensity Interval Training (LIIT) Breaks to Sitting

Detailed Description

The investigators are conducting an acute, single site randomized crossover trial testing two conditions lasting 3.5 hours each with a one to four-weeks washout period: (1) Interrupting sitting with 6 min of high-intensity interval training (HIIT) every 30 min, and (2) interrupting sitting with 6-min light-intensity interval training (LIIT) every 30 min. Investigators will administer the conditions in a counterbalanced order to 54 older adults (40-75 years). Brain function will be assessed using event-related brain potentials and measures of functional connectivity derived from electroencephalography. Cognitive functions will be assessed using a modified Eriksen flanker task and an antisaccade task. Investigators will measure episodic memory using a mnemonic discrimination task.

• Study Type: Interventional
• Enrollment (Estimated): 54
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Dominika M Pindus, Ph.D.; Phone Number: 217-300-7317; Email: pindus@illinois.edu

Study Locations

• United States
  • Illinois
    • Urbana, Illinois, United States, 61801
      • Recruiting
      • University of Illinois at Urbana-Champaign
      • Contact: Dominika M Pindus, PhD; Phone Number: 2173007317; Email: pindus@illinois.edu

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

1. Age 40-75 years
2. BMI <40 kg/m2
3. Sedentary (≥ 6 h/day sitting by a survey question)
4. Physically inactive adults based on the CSEP-PATH: Physical Activity and Sedentayr Behaviour Questionnaire (PASB-Q) Adult (low or medium physical activity range equivalent to less than 300 min of moderate intensity physical activity per week)
5. Capable of exercising vigorously based on the Physical Activity Readiness Questionnaire (PARQ+)
6. Has a medical clearance for maximal exercise and HIIT from a physician
7. Normotensive or participant's blood pressure is controlled
8. Intelligence quotient (IQ) ≥85
9. Fasting plasma glucose <126 mg/dL
10. Good or corrected vision and hearing
11. Right-handed
12. No significant abnormalities on the ECG during the maximal exercise test
13. No signs and symptoms that suggest an underlying cardiovascular disease as recorded during the maximal exercise test by a study physician.
14. No indications to prematurely stop the maximal exercise test as outlined by the ACSM's Guidelines for Exercise Testing and Prescription
15. Concussion if more than 12 months before the study screening.
16. History of cancer but in full remission for at least 12 months and no history of chemotherapy, signed off by the physician or an oncologist

Exclusion Criteria:

1. Physical disability or musculoskeletal disease prohibitive to vigorous exercise
2. Learning disabilities
3. Cognitive impairment (MoCA <26)
4. Type 1 or 2 diabetes
5. Neurological condition (e.g., Multiple Sclerosis, Parkinson, Dementia, Mild Cognitive Impairment)
6. Color blindness
7. Brain injury (e.g., traumatic brain injury, stroke, concussion)
8. Migraine headaches
9. Presence of other health conditions that may be exacerbated by exercise
10. History of heart disease
11. High cholesterol not controlled by medication
12. Signs and symptoms indicative of underlying cardiovascular disease
13. A chronic pulmonary disease
14. Emphysema
15. Pulmonary embolus
16. Asthma
17. History of renal disease
18. History of seizures
19. A neuropsychiatric disorder
20. Osteoporosis if it interferes with an individual's ability to exercise
21. Severe back problems
22. Severe arthritis if it interferes with an individual's ability to exercise
23. Thyroid disorder not controlled by medication
24. Polyneuropathy
25. Sleep disorders
26. Acquired immunodeficiency syndrome (AIDS)
27. Hepatitis C
28. History of long Coronavirus Disease 2019 (COVID-19)
29. History of cancer if not in full remission for at least 12 months and if a participant had the history of chemotherapy
30. Current or past smoking <12 months
31. Corticosteroid intake < 31 days before screening
32. Opioids taken < 6 months from screening
33. Anabolic androgens taken < 31 days before screening
34. A serious illness or hospitalization in the last six months
35. Currently taking medications that can affect the central nervous system (except for anxiolytics)
36. Current participation in an ongoing trial likely to influence exercise ability or cognitive function

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: High-Intensity Interval Training (HIIT) Breaks; Sitting interrupted every 30 min by 6-min HIIT bouts.	Behavioral: High-Intensity Interval Training (HIIT) Breaks to Sitting; HIIT Breaks: a 3.5-hour sitting will be interrupted every 30 min with 6-min HIIT Breaks. During sitting participants will engage in standardized sedentary activities. Each 6-min HIIT break comprises a one-minute warm-up, followed by a 2-minute high-intensity interval, one minute rest (sitting on a cycle ergometer) followed by another 2 minutes at high intensity.
Sham Comparator: Light-Intensity Interval Training (HIIT) Breaks; Sitting interrupted every 30 min by 6-min LIIT bouts.	Behavioral: Light-Intensity Interval Training (LIIT) Breaks to Sitting; LIIT Breaks: a 3.5-hour sitting will be interrupted every 30 min with 6-min LIIT Breaks. Each 6-min LIIT break comprises a one-minute warm-up, followed by a 2-minute light-intensity interval, one minute rest (sitting on a cycle ergometer) followed by another 2-minute light-intensity interval.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in task-evoked brain activity	EEG will be used to measure event-related brain potentials including the P3b-component during an inhibitory control task.	Immediately before intervention, 2 x during the intervention (50 min and 120 min into the intervention), and immediately after the intervention

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in cognitive functions	A modified Eriksen flanker task will be used to measure inhibitory control.	Immediately before intervention, 2 x during the intervention (50 min and 120 min into the intervention), and immediately after the intervention
Change in cognitive functions	An antisaccade tasks will be used to measure inhibitory control. A mnemonic discrimination task will be used to measure episodic memory.	Immediately before intervention and immediately after the intervention
Change in resting stated and task evoked brain activity	EEG will be used to measure functional connectivity at rest and during engagement in inhibitory control and episodic memory tasks.	Immediately before intervention and immediately after the intervention

Collaborators and Investigators

• Sponsor: University of Illinois at Urbana-Champaign
• Collaborators: National Institute on Aging (NIA); Northeastern University
• Investigators: Principal Investigator: Dominika M Pindus, Ph.D., University of Illinois Urbana-Champaign

Study record dates

Study Major Dates

• Study Start (Actual): May 7, 2024
• Primary Completion (Estimated): July 31, 2025
• Study Completion (Estimated): July 31, 2025

Study Registration Dates

• First Submitted: October 25, 2023
• First Submitted That Met QC Criteria: January 29, 2024
• First Posted (Actual): February 5, 2024

Study Record Updates

• Last Update Posted (Actual): March 25, 2025
• Last Update Submitted That Met QC Criteria: February 4, 2025
• Last Verified: February 1, 2025

More Information

Terms related to this study

• Keywords: Functional connectivity; Sitting; High-intensity interval training; Event-related potentials; Executive functions; Inhibitory control; Episodic memory
• Other Study ID Numbers: 23938; 1R21AG080411-01A1 (U.S. NIH Grant/Contract)

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No