Holotranscobalamin Remains Unchanged During Pregnancy

Holotranscobalamin Remains Unchanged During Pregnancy. Longitudinal Changes of Cobalamins and Its Binding Proteins During Pregnancy and Postpartum

Background and objective: Plasma cobalamins decrease during pregnancy but it is not fully elucidated how this is reflected in the total and cobalamin saturated transport proteins, transcobalamin (total TC, holoTC) and haptocorrin (total HC, holoHC). TC transports cobalamin into the cells. The function of HC is unknown, but in contrast to TC it binds both cobalamins and cobalamin analogues.

Design and methods: Healthy pregnant women (N=141) had blood samples drawn at 18th, 32nd, 39th gestation week and 8 weeks postpartum. The protein moiety of TC and HC (total and holo) was measured by in-house ELISA methods.

Study Overview

• Status: Completed
• Conditions: Vitamin B12 Deficiency

Detailed Description

Background and objective: Plasma cobalamins decrease during pregnancy but it is not fully elucidated how this is reflected in the total and cobalamin saturated transport proteins, transcobalamin (total TC, holoTC) and haptocorrin (total HC, holoHC). TC transports cobalamin into the cells. The function of HC is unknown, but in contrast to TC it binds both cobalamins and cobalamin analogues.

Design and methods: Healthy pregnant women (N=141) had blood samples drawn at 18th, 32nd, 39th gestation week and 8 weeks postpartum. The protein moiety of TC and HC (total and holo) was measured by in-house ELISA methods.

• Study Type: Observational
• Enrollment: 150

Contacts and Locations

Study Locations

• Denmark
  • Copenhagen, Denmark, 2100
    • Nils Milman

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 45 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Female

Description

Inclusion Criteria:

• Danish healthy Caucasian women >18 years of age with previous uncomplicated pregnancies and deliveries and presently with a normal pregnancy.

Exclusion Criteria:

• >4 cigarettes smoked per day,
• Treatment with vitamin B12, folic acid or acetyl salicylic acid,
• Impaired renal function and clinically significant vaginal haemorrhage before first visit as well as blood haemoglobin <6.4 mmol/L at first visit.

Study Plan

How is the study designed?

Collaborators and Investigators

• Sponsor: University of Aarhus
• Collaborators: University of Copenhagen
• Investigators: Principal Investigator: Nils Milman, MD, University of Copenhagen

Study record dates

Study Major Dates

• Study Start: January 1, 1995
• Study Completion (ACTUAL): December 1, 1997

Study Registration Dates

• First Submitted: April 27, 2007
• First Submitted That Met QC Criteria: April 27, 2007
• First Posted (ESTIMATE): April 30, 2007

Study Record Updates

• Last Update Posted (ESTIMATE): October 17, 2008
• Last Update Submitted That Met QC Criteria: October 16, 2008
• Last Verified: April 1, 2007

More Information

Terms related to this study

• Keywords: Pregnancy; cobalamin; holotranscobalamin; vitamin B12 analogues; reference interval
• Additional Relevant MeSH Terms: Nutrition Disorders; Avitaminosis; Deficiency Diseases; Malnutrition; Vitamin B Deficiency; Vitamin B 12 Deficiency
• Other Study ID Numbers: KA-93140