Study of the Effect of Eicosanoids on Contractile Activity of Pregnant Human Myometrium in Pathological Situation (EAU2)

Study of the Effect of Eicosanoids on Contractile Activity of Pregnant Human Myometrium in Pathological Situation.

Recent studies show that EET and 20-HETE have important biological effects, particularly in the vascular system. The investigators studied the effect of eicosanoids on the gravid rat uterus. The results suggest that 20-HETE had an tocolytics effect on gravid uterus. In the previous study, we demonstrated that the enzymes of the pathway of EET were present in human uterine tissues. Moreover, the addition of an inhibitor of degradation of EET had an tocolytic effect on the human myometrium, as the exogenous addition of 8.9, 14,15-EET and 20-HETE.

Objectives:

Primary objective: To compare the balance of different metabolic pathways of arachidonic acid (AA) of the pregnant human myometrium in pathological situations (preterm labor, uterine atony, prolonged pregnancy).

Specific objectives: i) To study the effect of derived from the AA on in vitro contractile activity of normal and pathological uterine tissues, and ii) detect and quantify the different sub-products of metabolism of AA in the uterine tissues (myometrium, fetal membranes and placenta).

The management of uterine contraction is in the heart of modern obstetrics year, yet the progress made in other specialties, based on the study of smooth muscle have not yet been transposed in obstetrics. A better understanding of systems for regulating the contraction is important in terms of 1) new physiological knowledge, but it could also be the source of 2) modification of strategies to take care of premature delivery (new Tocolytic), or 3) improving the efficiency of uterine muscle during delivery or 4) for treatment of patients with prolonged pregnancy.

Study Overview

• Status: Unknown
• Conditions: Obstetric Labor Complications; Prolonged Pregnancy

Detailed Description

It is a clinical study with a slope fundamental aims to examine the metabolic pathways of AA of human myometrium and their functional roles according to their clinical profile divided into three contractile pathological situations - threat of premature delivery, dynamic dystocia, prolonged pregnancy - and two groups of patients at term: a group before work and group work.

The sampling method. After birth, a biopsy will be perform from the lower segment of the uterus. After caesarean sections of membrane and placenta are collected.

The substances studied during isometric tension tests are part of the three degradation pathways of the AA.

1. new eicosanoids in cumulative dose (8,9-EET, 11,12-EET, 14,15-EET, 20-HETE), and in combination
2. enzyme inhibitors of the eicosanoids pathway (AUDA, MS-PPOH, DDMS), and the COX and LOX pathways (indomethacin), alone or in combination.

• Study Type: Observational
• Enrollment (Anticipated): 130

Contacts and Locations

• Study Contact: Name: Stéphanie Corriveau, BSc; Phone Number: 13875 819 346-1110; Email: stephanie.corriveau@usherbrooke.ca
• Study Contact Backup: Name: Simon Blouin, PhD; Phone Number: 13877 819 346-1110; Email: syblouin.chus@ssss.gouv.qc.ca

Study Locations

• Canada
  • Quebec
    • Sherbrooke, Quebec, Canada, J1H 5N4
      • Recruiting
      • Centre Hospitalier de l'Université de Sherbrooke

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Female

• Sampling Method: Non-Probability Sample

Study Population

women who will have a c-section at the CHUS

Description

Inclusion Criteria:

• all women who will have a c-section

Exclusion Criteria:

• induction of labor,
• child with malformation,
• birth weight less than 2500 grams or greater than 4500g

Study Plan

How is the study designed?

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort
EAU2; Women who will have a c-section at the CHUS

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
effect of eicosanoids on contractile activity of myometrium of pregnant women with pathological situations	during c-section

Secondary Outcome Measures

Outcome Measure	Time Frame
effect of enzymatic inhibitors on contractile activity of myometrium from pregnant women with pathological situations	during c-section
detection of enzymes from the different pathways	after c-section
quantification of eicosanoids in different tissues	after c-section

Collaborators and Investigators

• Sponsor: Université de Sherbrooke
• Collaborators: Fonds de la Recherche en Santé du Québec
• Investigators: Principal Investigator: Rousseau Éric, PhD, Université de Sherbrooke

Publications and helpful links

General Publications

• Corriveau S, Pasquier JC, Blouin S, Bellabarba D, Rousseau E. Chronic levothyroxine and acute T3 treatments enhance the amplitude and time course of uterine contractions in human. Am J Physiol Endocrinol Metab. 2013 Mar 1;304(5):E478-85. doi: 10.1152/ajpendo.00346.2012. Epub 2012 Dec 18.

Study record dates

Study Major Dates

• Study Start: May 1, 2009
• Primary Completion (Anticipated): July 1, 2015
• Study Completion (Anticipated): October 1, 2015

Study Registration Dates

• First Submitted: July 14, 2009
• First Submitted That Met QC Criteria: July 14, 2009
• First Posted (Estimate): July 15, 2009

Study Record Updates

• Last Update Posted (Estimate): March 4, 2015
• Last Update Submitted That Met QC Criteria: March 3, 2015
• Last Verified: March 1, 2015

More Information

Terms related to this study

• Keywords: pregnant women; eicosanoids; contractile activity; obstetrics complications
• Additional Relevant MeSH Terms: Pregnancy Complications; Obstetric Labor Complications; Pregnancy, Prolonged
• Other Study ID Numbers: 09-040