Determination of Adipocytokine in Synovial Fluid of Osteoarthritis Patients

Determination of Adipocytokine in Synovial Fluid of Osteoarthritis Patients With Joint Pain and Effusion

The purpose of this study is to determine the concentration of different adipocytokine (adiponectin, leptin, resistin, visfatin, osteopontin) and an inflammation classic markers as Interleukine 1 beta, tumor necrosis alfa, interleukine 6 and high sensibility reactive c protein in the synovial fluid of knee osteoarthritis patients, and compare with the inflammation detected with ultrasonography evaluation of the joint. In last years, some studies appeared focusing on the role of adipocytokines in osteoarthritis and they correlated adipocytokines with cartilage degradation and x-ray degree; in our study we will focused in the importance of adipocytokines in the inflammatory changes more than in joint destruction.

Study Overview

• Status: Unknown
• Conditions: Osteoarthritis
• Intervention / Treatment: Other: arthrocentesis; Other: ultrasonography of the knee

• Study Type: Observational
• Enrollment (Anticipated): 35

Contacts and Locations

Study Locations

• Spain
  • Barcelona
    • Sabadell, Barcelona, Spain, 08208
      • Recruiting
      • Hospital Parc Tauli Sabadell
      • Sub-Investigator: Noemi Navarro, Dra

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 50 years to 81 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

Patient with knee osteoarthritis and synovial effusion who attende to a Rheumatologist unit for this problem. We made the visit and anonimously we kept the results of examination and ultrasonography in our database and we freezed synovial fluid. Now we will determine the adipocytokines and classic inflammatory parameters in this synovial fluid

Description

Inclusion Criteria:

• aged 50 to 81
• primary osteoarthritis of the knee
• synovial effusion in examination
• patient who have the results in our database

Exclusion Criteria:

• any other rheumatic conditions as rheumatoid arthritis or another inflammatory disease
• secondary knee osteoarthritis
• history of knee surgery
• any conditions that at investigator criteria exclude the patient from study procedures

Study Plan

How is the study designed?

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort

Intervention / Treatment

Inflammatory knee osteoarthritis; We recruited patients with osteoarthritis and synovial effusion to diagnostic the effusion and to confirm that this this synovial fluid had mechanical characteristics. Now we are going to determine the adipocytokines and traditional parameters of inflammation in this synovial fluid to correlate it, and we will associate it with the ultrasound explanation of the knee that we have in our database.

Other: arthrocentesis; The made a diagnostic arthrocentesis to all patient we included; Other: ultrasonography of the knee; We explored the affected knee with an ultrasound explanation in order to describe inflammatory changes based on standard examination.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Determination of adipocytokine levels (adiponectin, leptin, resistin, visfatin, osteopontin) in synovial fluid	We have all the synovial fluid freeze, so now we have to determine the levels of everyone.	We determine adipocytoquines in one month, all patients in the same determination kit

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Knee inflammation parameters detected by ultrasonography	We performed the ultrasonography evaluation at the same time than the visit were realized, and we have the results in a data base, so we will wait for the adipocytokine results and we will compare with ultrasound evaluation	three months
To determine additional classic parameters of inflammation in synovial fluid as Interleukine 1 beta, Interleukine 6 and tumor necrosis factor alfa	We will use classic inflammation parameters to compare with adipocytokine levels	We determine this inflammatory parameters in one month in all patient at the same time

Collaborators and Investigators

• Sponsor: Hospital Parc Taulí, Sabadell
• Investigators: Principal Investigator: Joan Calvet, Dr, Hospital Parc Tauli; Study Director: Cristobal Orellana, Dr, Hospital Parc Tauli

Study record dates

Study Major Dates

• Study Start: October 1, 2013
• Primary Completion (Anticipated): November 1, 2013
• Study Completion (Anticipated): March 1, 2014

Study Registration Dates

• First Submitted: November 8, 2013
• First Submitted That Met QC Criteria: November 19, 2013
• First Posted (Estimate): November 25, 2013

Study Record Updates

• Last Update Posted (Estimate): November 25, 2013
• Last Update Submitted That Met QC Criteria: November 19, 2013
• Last Verified: November 1, 2013

More Information

Terms related to this study

• Keywords: osteoarthritis; inflammation; ultrasonography; adipocytokines
• Additional Relevant MeSH Terms: Joint Diseases; Musculoskeletal Diseases; Rheumatic Diseases; Arthritis; Osteoarthritis
• Other Study ID Numbers: Joan03