Expandability of Aging Adipose Tissue (EAT)

June 11, 2019 updated by: University of Colorado, Denver

Aging Subcutaneous Adipose Tissue Response to Overfeeding

The purpose of this pilot study is to determine whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. The investigators hypothesize that the adipose tissue response to overfeeding may be weakened in older when compared to younger men, thereby leading to worsened ectopic storage depots for older men.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Detailed Description

This pilot study will begin to address whether adipose tissue biology changes with advancing age. Specifically, we will test whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. To test this possibility the Investigators will recruit overweight and mildly obese older (age 55-75y) and younger (age 25-40y) men and collect adipose tissue biopsies before and after 7 days of overfeeding. The investigators will test how well the cells in adipose tissue expand in response to overfeeding and whether this differs between younger and older men.

Study Type

Interventional

Enrollment (Actual)

20

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • United States
    • Colorado
      • Aurora, Colorado, United States, 80045
        • Univeristy of Colorado, Anschutz Medical Campus

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

25 years to 75 years (ADULT, OLDER_ADULT)

Accepts Healthy Volunteers

Yes

Genders Eligible for Study

Male

Description

Inclusion Criteria:

  • Men
  • Aged 25-40y OR 55-75y
  • BMI 28-35 kg/m2
  • Weight stable (±2kg over past 3 mo)
  • Sedentary to moderately active (<3 d/wk of exercise lasting >20min)
  • Non-smokers (never used or quit >7yrs prior)

Exclusion Criteria:

  • Women
  • Currently using hormones, glucose or lipid lowering medication
  • Engaged in exercise or diet program
  • Currently gaining or losing weight or using weight loss drugs
  • Diabetes mellitus (diagnosis, treatment, FBG ≥126 mg/dL)
  • Uncontrolled metabolic disorders (e.g., thyroid)

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: BASIC_SCIENCE
  • Allocation: NON_RANDOMIZED
  • Interventional Model: SINGLE_GROUP
  • Masking: NONE

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
OTHER: MA
Middle-aged overweight and obese men (age 25-40yr)
Other: Overfeeding
Consumption of 40% excess calories for 7 consecutive days
OTHER: OA
Older overweight and obese men (age 55-75yr)
Other: Overfeeding
Consumption of 40% excess calories for 7 consecutive days

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Change in adipose-derived stem cell (ASC) differentiation (Delta%oil red O+)
Time Frame: 1 wk of overfeeding
age-related difference in the ASC differentiation response to overfeeding
1 wk of overfeeding

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Change in ASC proliferation (Delta rate of cell growth)
Time Frame: 1 wk of overfeeding
age-related difference in the ASC proliferative response to overfeeding
1 wk of overfeeding
Change in expression of C/EBPalpha, C/EBPbeta, PPARgamma2, SREBP1c, SREBf1a, and Wnt10b (fold-changes in mRNA expression)
Time Frame: 1 wk overfeeding
age-related difference in the pro-adipogenic response to overfeeding
1 wk overfeeding
Change in intramyocellular (IMCL) di-saturated diacylglycerol (DAG) localization (Delta %membrane:cytosol)
Time Frame: 1 week overfeeding
age-related difference in the IMCL DAG response to overfeeding
1 week overfeeding

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

University of Colorado, Denver

Collaborators

Colorado State University

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start

June 1, 2015

Primary Completion (ACTUAL)

December 1, 2016

Study Completion (ACTUAL)

December 1, 2018

Study Registration Dates

First Submitted

April 8, 2015

First Submitted That Met QC Criteria

May 26, 2015

First Posted (ESTIMATE)

May 29, 2015

Study Record Updates

Last Update Posted (ACTUAL)

June 13, 2019

Last Update Submitted That Met QC Criteria

June 11, 2019

Last Verified

June 1, 2019

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • 14-2411

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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