Physiological Effect of Ataulfo Mango (Manguifera Indica) Beverage in Exercise

Physiological Response to Ataulfo Mango Pulp and Peel Beverages in Exercise

The aim of this study is to assess the antioxidant capacity of two mango base beverages (AM1, AM2), against exercise-induced oxidative stress.

Study Overview

• Status: Completed
• Conditions: Exercise-induced Oxidative Stress
• Intervention / Treatment: Dietary supplement: Mango pulp beverage; Dietary supplement: Mango pulp and peel beverage; Dietary supplement: Control beverage

Detailed Description

Introduction. The post-consumption physiological effect of mango cv. "Ataulfo" pulp (MP) and peel (MC) in humans before exercise has been poorly evaluated.

Objective. To evaluate the physiological response, to consumption of two mango base beverages, AM1 (200g MP/ 600mL water) and AM2 (160g MP; 40g MC / 600mL water), before and after a submaximal exercise test.

Methods. The antioxidant profile and physicochemical characteristics of AM1 and AM2 were evaluated. Several cardiorespiratory, anthropometric and body composition parameters of 19 university students, prior to the consumption of AM1, AM2 and water, were evaluated in three non-consecutive sessions (self-control cases). The glycemic, lactic and antioxidant (FRAP), uric acid (AU), reduced glutathione (GSH) and lipid oxidation (TBARS) and protein carbonyls (PC) responses were evaluated in plasma before and after an incremental cycle ergometer exercise test.

• Study Type: Interventional
• Enrollment (Actual): 19
• Phase: Not Applicable

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 17 years to 28 years (Child, Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• healthy male
• university student
• no alcohol, cigar or drugs consumption

Exclusion Criteria:

• no antioxidant supplement or ergogenic consumption
• health issues with performing physical activity

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: AM1; Mango pulp beverage	Dietary supplement: Mango pulp beverage; The subject consumes mango pulp beverage (200 g mango pulp + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.; Other Names: AM1; 200 g mango pulp + 600 mL water
Experimental: AM2; Mango pulp and peel beverage	Dietary supplement: Mango pulp and peel beverage; The subject consumes mango pulp and peel beverage (160 g mango pulp + 40 g mango peel + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.; Other Names: AM2; 160 g mango pulp + 40 g mango peel + 600 mL water
Placebo Comparator: Control; Control beverage	Dietary supplement: Control beverage; The subject consumes control beverage (600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.; Other Names: 600 mL water

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Antioxidant capacity change	Assessed by FRAP change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 min (immediately after exercise test)
Antioxidant capacity change	Assessed by uric acid concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 (immediately after exercise test)
Antioxidant capacity change	Assessed by reduced glutathione concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 (immediately after exercise test)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Oxidative stress change	Assessed by TBARS concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 (immediately after exercise test)
Oxidative stress change	Assessed by protein carbonyls concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 (immediately after exercise test)
Lactic acid concentration change	Assessed by lactic acid concentration change. Capillary blood samples were taken from the fingertip, using a lancet and analyzed (YSI modelo 1500, Yellow Springs, USA)	0 min (before performing exercise test), 3 min, 6 min, 9 min, 12 min, 15 min (during exercise test)
Postprandial glycemic change	Assessed by blood glucose concentration change. Capillary blood samples were taken from the fingertip, using a lancet, and analyzed (ReliOn Confirm/micro Test Strips ARKRAY, USA)	0 min (before beverage intake), 30 min, 60 min, 90 min, 120 min (after beverage intake)

Collaborators and Investigators

• Sponsor: Universidad Autonoma de Ciudad Juarez
• Investigators: Study Director: Abraham Wall-Medrano, PhD, Universidad Autonoma de Ciudad Juarez; Principal Investigator: Gregorio Chavez-Treviño, MS, Universidad Autonoma de Ciudad Juarez

Publications and helpful links

General Publications

• Alvarez-Parrilla E, De La Rosa LA, Legarreta P, Saenz L, Rodrigo-Garcia J, Gonzalez-Aguilar GA. Daily consumption of apple, pear and orange juice differently affects plasma lipids and antioxidant capacity of smoking and non-smoking adults. Int J Food Sci Nutr. 2010 Jun;61(4):369-80. doi: 10.3109/09637480903514041.
• Ramos-Jimenez A, Hernandez-Torres RP, Wall-Medrano A, Torres-Duran PV, Juarez-Oropeza MA, Solis Ceballos JA. Acute physiological response to indoor cycling with and without hydration; case and self-control study. Nutr Hosp. 2013 Sep-Oct;28(5):1487-93. doi: 10.3305/nh.2013.28.5.6698.
• Spirlandeli AL, Deminice R, Jordao AA. Plasma malondialdehyde as biomarker of lipid peroxidation: effects of acute exercise. Int J Sports Med. 2014 Jan;35(1):14-8. doi: 10.1055/s-0033-1345132. Epub 2013 Jun 14.
• Wang CY, Haskell WL, Farrell SW, Lamonte MJ, Blair SN, Curtin LR, Hughes JP, Burt VL. Cardiorespiratory fitness levels among US adults 20-49 years of age: findings from the 1999-2004 National Health and Nutrition Examination Survey. Am J Epidemiol. 2010 Feb 15;171(4):426-35. doi: 10.1093/aje/kwp412. Epub 2010 Jan 15.
• Wall-Medrano A, Olivas-Aguirre FJ, Velderrain-Rodriguez GR, Gonzalez-Aguilar A, de la Rosa LA, Lopez-Diaz JA, Alvarez-Parrilla E. [Mango: agroindustrial aspects, nutritional/functional value and health effects]. Nutr Hosp. 2014 Nov 1;31(1):67-75. doi: 10.3305/nh.2015.31.1.7701. Spanish.

Study record dates

Study Major Dates

• Study Start (Actual): January 1, 2017
• Primary Completion (Actual): February 28, 2017
• Study Completion (Actual): April 30, 2017

Study Registration Dates

• First Submitted: May 29, 2017
• First Submitted That Met QC Criteria: June 7, 2017
• First Posted (Actual): June 9, 2017

Study Record Updates

• Last Update Posted (Actual): June 9, 2017
• Last Update Submitted That Met QC Criteria: June 7, 2017
• Last Verified: June 1, 2017

More Information

Terms related to this study

• Keywords: Oxidative stress; Exercise; Antioxidant
• Other Study ID Numbers: UACJ-ICB-2017-01

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No