Comparing Acute Aerobic and Resistance Exercise (CAARE)

Effects of Age, Fitness Level, and Modality on Physiological Response to Acute Exercise

The purpose of this study is to determine the effect of age and fitness level on the physiological response to an acute bout of resistance or aerobic exercise.

Study Overview

• Status: Terminated
• Conditions: Exercise Physiology
• Intervention / Treatment: Other: Resistance training exercise; Other: Aerobic training exercise

Detailed Description

Young and middle-aged to older adults who are physically active or not physically active will participate in two moderate to vigorous intensity exercise bouts: aerobic exercise and resistance training exercise. Blood will be collected to assess immune function and metabolism.

• Study Type: Interventional
• Enrollment (Actual): 35
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Texas
    • Houston, Texas, United States, 77204
      • University of Houston

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 75 years (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• "Young" Adults: ages 18-30
• "Older" Adults: ages 55-75 and if a female postmenopausal
• Participants classified as "trained" must participate in at least 30 minutes of moderate to vigorous intensity cardiovascular activity at least 3 days per week and engage in resistance training for all of the major muscle groups approximately 2 days / week. They must have upheld this activity for the past 3 months
• Participants classified as "untrained" must be participating in less than 30 minutes of moderate to vigorous intensity cardiovascular activity and less than 2 days of resistance training per week for at least the last 3 months.

Exclusion Criteria:

• Have any contraindications to moderate to vigorous exercise
• Range of motion restrictions that would prevent them from participating in aerobic or resistance training with proper form (they must be ambulatory)
• aking medications (prescription or over the counter) known to influence immune function, including daily NSAID's, beta blockers, statins, bisphosphonates, or steroids.
• Have known cardiovascular, respiratory, metabolic, or renal disease, with the exception of controlled hypertension (as defined by resting BP below 140/90) and/or controlled asthma (self-reported).
• Be pregnant
• Be unable to complete all visits (i.e. must not be planning to leave the Houston area long-term before concluding their participation in the study which will span several weeks)
• Fall outside of a BMI range of (18.5 - 30)
• Consume alcohol or recreational drugs for 24h prior to visits
• Older women cannot be pre-menopausal (must have not had a menses for at least 12 months)
• Unable to speak or read English
• Participants must not have scheduling conflicts that would prevent them from reporting to the laboratory of integrated physiology 4 times over the course of the study

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Supportive Care
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: resistance training exercise; All participants complete one session of resistance training exercise	Other: Resistance training exercise; randomized complete crossover for acute resistance training exercise session
Experimental: aerobic training exercise; All participants complete one session of aerobic training exercise	Other: Aerobic training exercise; randomized complete crossover for acute aerobic exercise training exercise session

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Monocyte subset and function	Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function	Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise
Monocyte subset and function	Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function	Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise
Monocyte subset and function	Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function	compare pre-RT to pre-AT
Monocyte subset and function	Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function	compare immediately post-RT to immediately post-AT
Monocyte subset and function	Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function	compare 1hr post-RT to 1hr post-AT
NK cell function	Flow cytometry to identify NK cells and in vitro activity to assess NK activity	Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise
NK cell function	Flow cytometry to identify NK cells and in vitro activity to assess NK activity	Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise
NK cell function	Flow cytometry to identify NK cells and in vitro activity to assess NK activity	compare pre-RT to pre-AT
NK cell function	Flow cytometry to identify NK cells and in vitro activity to assess NK activity	compare immediately post-RT to immediately post-AT
NK cell function	Flow cytometry to identify NK cells and in vitro activity to assess NK activity	compare 1hr post-RT to 1hr post-AT
Circulating relative and absolute counts of T cell subsets	Flow cytometry to identify T cell subsets	Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise
Circulating relative and absolute counts of T cell subsets	Flow cytometry to identify T cell subsets	Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise
Circulating relative and absolute counts of T cell subsets	Flow cytometry to identify T cell subsets	compare pre-RT to pre-AT
Circulating relative and absolute counts of T cell subsets	Flow cytometry to identify T cell subsets	compare immediately post-RT to immediately post-AT
Circulating relative and absolute counts of T cell subsets	Flow cytometry to identify T cell subsets	compare 1hr post-RT to 1hr post-AT

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Serum cortisol	ELISA to measure circulating cortisol (serum)	Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise
Serum cortisol	ELISA to measure circulating cortisol (serum)	Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise
Serum cortisol	ELISA to measure circulating cortisol (serum)	compare pre-RT to pre-AT
Serum cortisol	ELISA to measure circulating cortisol (serum)	compare immediately post-RT to immediately post-AT
Serum cortisol	ELISA to measure circulating cortisol (serum)	compare 1hr post-RT to 1hr post-AT

Collaborators and Investigators

• Sponsor: University of Houston
• Investigators: Principal Investigator: Melissa Markofski, PhD, University of Houston

Publications and helpful links

General Publications

• Graff RM, Jennings K, LaVoy ECP, Warren VE, Macdonald BW, Park Y, Markofski MM. T-cell counts in response to acute cardiorespiratory or resistance exercise in physically active or physically inactive older adults: a randomized crossover study. J Appl Physiol (1985). 2022 Jul 1;133(1):119-129. doi: 10.1152/japplphysiol.00301.2021. Epub 2022 May 26.

Study record dates

Study Major Dates

• Study Start (Actual): December 18, 2018
• Primary Completion (Actual): March 31, 2020
• Study Completion (Actual): March 31, 2020

Study Registration Dates

• First Submitted: December 17, 2018
• First Submitted That Met QC Criteria: January 2, 2019
• First Posted (Actual): January 4, 2019

Study Record Updates

• Last Update Posted (Actual): March 24, 2023
• Last Update Submitted That Met QC Criteria: March 22, 2023
• Last Verified: March 1, 2023

More Information

Terms related to this study

• Other Study ID Numbers: STUDY00001191

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: UNDECIDED
• IPD Plan Description: We are considering making available IDP to others upon request, but would have to get an IRB change because that is not in our protocol

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No