Two-day Bed Rest, Insulin Sensitivity and Muscle Protein Synthesis (2d-BR)

The Impact of Two Days of Bed Rest Versus Two Days of Habitual Activity on Insulin Sensitivity and Cumulative Muscle Protein Synthesis in Healthy Young Males

The impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males

Study Overview

• Status: Withdrawn
• Conditions: Healthy
• Intervention / Treatment: Behavioral: Habitual activity; Behavioral: Bed rest

Detailed Description

To study the mechanisms underlying inactivity-induced muscle atrophy, the impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males

• Study Type: Interventional
• Phase: Not Applicable

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Male
• Aged 18-40

Exclusion Criteria:

• Smoker
• Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes)
• Any diagnosed cardiovascular disease (e.g. deep vein thrombosis or hypertension)
• Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism).
• A personal or family history of thrombosis, epilepsy, seizures or schizophrenia.
• Any known disorders in muscle metabolism
• Regular use of nutritional supplements (i.e. ingestion more than twice per week during the last month)
• Metallic implants (including heart pacemaker, cochlear implants, medication pumps, surgical clips, plates or screws).
• Allergy to lidocaine
• Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture)

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Habitual activity and 2 days bed rest; Participants will undergo 2 days of habitual activity and 2 days bed rest	Behavioral: Habitual activity; Two days of habitual physical activity, under normal free-living conditions; Behavioral: Bed rest; Two days of bed rest, to induce whole-body physical inactivity

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in whole-body insulin sensitivity	Change in whole-body insulin sensitivity via meal tolerance test	During 2 days of habitual physical activity and 2 days of bed rest. Blood glucose and insulin are monitored for 2 hours following ingestion of a standardized meal

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in cumulative muscle protein synthesis	Cumulative muscle protein synthesis via deuterium oxide ingestion. The incorporation of deuterated alanine in muscle is expressed relative to body water enrichments, to obtain muscle protein synthesis in %/day	During 2 days of habitual physical activity and 2 days of bed rest
Change in continuous glucose	Change in continuous glucose via continuous glucose monitoring.	Continuous glucose, during the 2 days of habitual activity and 2 days of bed rest
Change in muscle volume	Change in muscle volume via MRI	Immediately before and immediately after the 2-day bed rest period

Collaborators and Investigators

• Sponsor: University of Exeter
• Investigators: Principal Investigator: Marlou L Dirks, PhD, University of Exeter

Study record dates

Study Major Dates

• Study Start (Actual): September 1, 2021
• Primary Completion (Actual): September 1, 2021
• Study Completion (Actual): September 1, 2021

Study Registration Dates

• First Submitted: February 28, 2019
• First Submitted That Met QC Criteria: March 14, 2019
• First Posted (Actual): March 18, 2019

Study Record Updates

• Last Update Posted (Actual): November 17, 2021
• Last Update Submitted That Met QC Criteria: November 9, 2021
• Last Verified: November 1, 2021

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Glucose Metabolism Disorders; Metabolic Diseases; Hyperinsulinism; Insulin Resistance
• Other Study ID Numbers: 190206/B/05

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No