- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04194567
Effect of Polyphenols on Iron Absorption From Finger Millet
An Absorption Study in Young Women to Evaluate the Effect of Polyphenols on Iron Absorption From Ragi (Finger Millet) Using the Iron Isotopic Method
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
Finger Millet (Eleusine coracana), more commonly known as Ragi, is a type of a millet crop grown in India, including other countries especially in East Africa. Ragi has been perceived as a potential "super cereal" by the United States National Academies being one of the most nutritious among all major cereals. From the nutritional perspective, finger millet is considerably rich in minerals and its micronutrient density is higher than that of the world's major cereal grains: rice and wheat .
Millets (Ragi or finger millet and Jowar or sorghum) were consumed by 59% of rural and 28% of urban population in Karnataka. Ragi consumption was seen predominantly in south Karnataka with 27% of rural and 17% of urban population reporting consumption.
Ragi is the richest source of calcium among cereals with up to 10-fold higher calcium content than brown rice, wheat or maize and three times than that of milk. It is also rich in iron and fiber, making this crop more nutritive as compared to other most commonly used cereals. Finger millet is enriched in the essential amino acids like lysine and methionine which are important in human health and growth but remain absent from most other plant foods . In addition, it also contains useful amounts of the two polyunsaturated fatty acids- linoleic acid and α-linolenic acid , metabolized products of which facilitate normal development of the central nervous system. Despite the well-documented health benefits of finger millet, only a limited progress has been made in context to its nutraceutical applications as functional food. Although, it may have remained underutilized by the general population due to either unawareness or apprehension, its immense potential for enhancing the nutritional and therapeutic attributes by acting as functional food cannot be ignored.
Ragi also contains different antinutrients such as phytates and polyphenols, which have been known to have an inhibiting effect on the absorption of iron. On an average, white finger millet contains 0.04-0.09% polyphenols and brown varieties have 0.08-3.47% . It was found that found proto-catechuic acid (45.0 mg/100 g) is the major free phenolic acid in ragi. In another study of total phenolic compounds in finger millet, it was found to have 85% benzoic acid derivatives (gallic acid, protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, syringic acid) while rest of the fraction were either cinnamic acid derivatives (ferulic acid, trans-cinnamic acid, p-coumaric acid, caffeic acid, sinapic acid) or flavonoids (quercetin, Proanthocyanidins like condensed tannins) . Among bound phenloic acids, ferulic and p-coumaric acid are the major fractions and account for 64-96 and 50-99% of total ferulic and p-coumaric acid content of finger millet grains, respectively.
The dark variety of ragi is the most widely consumed type. The white ragi is the lesser consumed type. However, the levels of polyphenols, which inhibit the absorption of iron, are ten times lower in the white ragi as compared to the dark ragi. Thus, it is important to evaluate the extent of inhibition of the polyphenols on the iron absorption. This information would be beneficial for policy making and promotion of cultivation of the type of ragi that could be most effective for the consumers
The study duration will be 26 days from the first day of the first meal administration till the last blood withdrawal . For each appointment, the participants will spend about 30 minutes at the Human Nutrition Laboratory at ETH Zurich, between 7 and 9 am for breakfast meal consisting of pancakes made out of the ragi. the white ragi meal and the black ragi meal will be extrinsically labelled with isotopes 57 Fe and 58 Fe respectively. The participants will consume the ragi meal as breakfast. The first type of ragi meal will be given for 5 consecutive days,. They will have a weekend break of two days (during which they will resume their usual eating habits). After that, they will consume the second test meal as breakfast for another five consecutive days. Thus, there will be 10 meals in total. The breakfast meals will be consumed between 7 and 9 am together with a glass of 300 mL bottled water after which they have to wait another 3 hours before they can consume anything. The procedure will remain the same for all the days. Participants will always be asked to fast the day before coming for the feeding, that is, no food intake after 8 pm and no beverage intake after midnight the evening before. On day of screening, day 1 and the end point day 26 of the study, blood samples will be collected after an overnight fast for the determination of Hb, PF, CRP and isotopic ratio
Study Type
Enrollment (Actual)
Phase
- Not Applicable
Contacts and Locations
Study Locations
-
-
-
Zurich, Switzerland, 8092
- ETH Zurich, Laboratory of Human Nutrition
-
-
ZH
-
Zürich, ZH, Switzerland, 8092
- ETH Zurich, Laboratory of Human Nutrition
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- Female, 18 to 40 years old
- Body weight up to 65 kg
- Normal Body Mass Index (18.5 - 25 kg/m2)
- Signed informed consent
- Plasma ferritin < 25 ng/ml
- Able to communicate in and comprehend English language
- Tasted and found the Ragi to be of acceptable taste and confirmed will to consume the test meals if enrolled in the study
Exclusion Criteria:
- Pregnancy (assessed by a urinary pregnancy test) / intention to become pregnant
- Lactating up to 6 weeks before study initiation
- Moderate or severe anaemia (Hb < 9.0 g/dL)
- Elevated CRP (> 5.0 mg/L)
- Any metabolic, gastrointestinal, kidney or chronic disease such as celiac disease, diabetes, hepatitis, hypertension, cancer or cardiovascular diseases (according to the participants own statement)
- Continuous/long-term use of medication during the whole study (except for contraceptives)
- Consumption of mineral and vitamin supplements within 2 weeks prior to first meal administration
- Blood transfusion, blood donation or significant blood loss (accident, surgery) within 4 months prior to first meal administration
- Earlier participation in a study using iron stable isotopes or participation in any clinical study within the last 30 days
- Participant who cannot be expected to comply with study protocol (e.g. not available on certain study appointments or difficulties with blood withdrawal)
- Inability to understand the information sheet and the informed consent form due to cognitive or language reasons
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Other
- Allocation: Randomized
- Interventional Model: Crossover Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: Black Ragi
During one week of the study, participants are to consume pancakes made from the dark variety of ragi.
|
Pancakes made out of the dark ragi
|
Experimental: White Ragi
During the second week of the study, participants are to consume pancakes made from the white variety of ragi
|
Pancakes made out of the white ragi
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Fractional Iron absorption
Time Frame: 26th day of the study
|
The fractional iron absrption will be compared in predefined comparisons from the two interventions products that are administered to the study participants
|
26th day of the study
|
Total Iron Absorption
Time Frame: 26th day of the study
|
Total iron absorption from the two ragi types will be compared by measuring the total iron absorbed from all the meals in the study
|
26th day of the study
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Hemoglobin
Time Frame: screening (-1), baseline (day 1), last test meal consumption (day 26)
|
Iron status marker
|
screening (-1), baseline (day 1), last test meal consumption (day 26)
|
Plasma ferritin
Time Frame: screening (-1), baseline (day 1), last test meal consumption (day 26)
|
Iron status marker
|
screening (-1), baseline (day 1), last test meal consumption (day 26)
|
C-Reactive Protein
Time Frame: screening (-1), baseline (day 1), last test meal consumption (day 26)
|
Inflammation status
|
screening (-1), baseline (day 1), last test meal consumption (day 26)
|
Body Iron stores
Time Frame: last test meal consumption (day 26)
|
Iron status marker
|
last test meal consumption (day 26)
|
Soluble transferrin receptor
Time Frame: last test meal consumption (day 26)
|
Iron status marker
|
last test meal consumption (day 26)
|
Collaborators and Investigators
Collaborators
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- Ragi
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
Clinical Trials on Iron-deficiency
-
Pennington Biomedical Research CenterRecruitingIron-deficiency | Iron Deficiency Anemia | Iron Deficiency Anemia TreatmentUnited States
-
King's CollegeCompletedIron-deficiency | Iron Deficiency Anemia | Iron Deficiency (Without Anemia)United States
-
Children's Hospital Los AngelesNot yet recruitingAnemia | Iron Deficiency Anemia | Anemia, Iron Deficiency | IDA - Iron Deficiency AnemiaUnited States
-
Swiss Federal Institute of TechnologyUnited States Agency for International Development (USAID); Quadram Institute... and other collaboratorsCompletedIron-deficiency | Iron Deficiency Anemia | Iron Deficiency (Without Anemia)Peru
-
Arrowhead Regional Medical CenterRecruitingIron Deficiency Anemia of PregnancyUnited States
-
Société des Produits Nestlé (SPN)CompletedIron-deficiency | Anemia | Iron Deficiency AnemiaPhilippines
-
Swiss Federal Institute of TechnologyUniversity of MalawiCompletedIron-deficiency | Iron Deficiency AnemiaMalawi, Switzerland
-
Johann Wolfgang Goethe University HospitalLudwig-Maximilians - University of MunichCompletedNon-invasive Diagnostics of Iron Deficiency in Surgical Patients by Measuring Zinc Protoporphyrin-IXIron-deficiency | Anemia | Iron Deficiency AnemiaGermany
-
Kansas State UniversityUnited States Department of Agriculture Foreign Agricultural Service; American...CompletedIron-deficiency | Iron Deficiency AnemiaUnited States
-
Baylor College of MedicineNational Heart, Lung, and Blood Institute (NHLBI)CompletedIron-deficiency | Iron Deficiency AnemiaUnited States
Clinical Trials on Black Ragi
-
Weill Medical College of Cornell UniversityNational Institute on Minority Health and Health Disparities (NIMHD); Cornell...RecruitingObesity, Childhood | Type2DiabetesUnited States
-
Ramsar international campusCompleted
-
University of Massachusetts, AmherstCompletedAbsorption | Black Pepper | CarotenoidUnited States
-
Rush University Medical CenterNational Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); University... and other collaboratorsRecruitingDiabetes Mellitus | Adolescent ObesityUnited States
-
University of California, San FranciscoNational Institute of Mental Health (NIMH)Completed
-
The Center for Applied Health Sciences, LLCBrightseed, IncCompletedInflammation | Triglycerides | Appetite | Lipids | Waist CircumferenceUnited States
-
University of Medicine and Pharmacy "Victor Babes...ASCAR Cardiology Clinic, Timisoara City HospitalActive, not recruitingEvaluation of Black Chokeberry Bio Juice on Blood Pressure Levels and Endothelial Damage (AROBLENDO)Inflammation | Essential Hypertension | Endothelial DysfunctionRomania
-
Natural Immune Systems IncNew Chapter Inc.CompletedHyperglycemia | HyperlipidemiasUnited States
-
The Ottawa HospitalWithdrawnBreast Cancer | Axillary LymphadenitisCanada
-
Ohio State UniversityAmerican Heart AssociationRecruitingCardiovascular Diseases | Smoking | Hypertension | Obesity | Sleep | PreDiabetes | Type 2 Diabetes | Blood Pressure | Hyperlipidemias | Diet, Healthy | Physical Inactivity | Cardiometabolic SyndromeUnited States