Natural Antioxidant Intake After Exercise Can Oxidative Stress in the Third Trimester of Pragnancy (MDA)

January 14, 2020 updated by: Dr. Ocktariyana, SST, M.Kes, Indonesia University

Natural Antioxidant Intake After Exercise Can Reduce Malondialdehyde (MDA) Level in the Third Trimester of Pragnancy

Pregnancy is a condition that increased requirements of energy and oxygen, and if this condition is not maximally that can lead oxidative stress and produce free radicals which is affect in the pregnancy process. Malondialdehyde (MDA) level in pregnant women is higher than non-pregnant women. Exercise is one way to neutralize the free radical. The aim of this study was analyzed effect of treatment natural antioxidants after exercise which was pregnancy workout to MDA level in the third trimester of pregnancy.

This study was a true experimental study. There were 24 prenatal pregnant women and separated to 3 groups, which were 8 prenatal pregnant women without pregnancy workout as a control group, 8 prenatal pregnant women with pregnancy workout but without natural antioxidant consume as a positive control group, and 8 prenatal pregnant women with natural antioxidant consume after pregnancy workout as a treatment group. The antioxidant compounds given in this study were guava juice that contains vitamin C. MDA levels was analyzed by spectrophotometric. Comparison between groups was done by one way ANOVA test.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Detailed Description

Pregnancy is a condition that is vulnerable to all kinds - stress resulting in changes in physiology and metabolic function. In pregnancy there is also an increase in energy and oxygen requirements.

The metabolic process can increase require of the oxygen and if it is not available as well as unmaximal used can causes the formation of oxidative stress and produces excessive free radicals which ultimately, that affected in the saferity of a pregnancy process. The imbalance between antioxidants and free radicals in pregnancy prosses are causes pathological and complications changes in pregnancy. This kind of biochemical marker of antioxidants and oxidants is very useful in observing complications that may arise in pregnancy.

Treatment given is expected to eliminate complaints that arise while exercise during pregnancy can prevent the formation of free radicals, but exercise must be given in accordance with the ability of pregnant women.

Physical exercise or exercise in pregnancy can maintain excess weight gain, prevent diabetes, hypertension, and shorten labor time. Sports is one way to neutralize the formation of free radicals because by doing physical activity, excessive oxygen can be used to produce energy.

Increased free radicals are characterised by an increase of malondialdehyde (MDA) as a biochemical markers. Malondialdehyde (MDA) which is the output of lipid peroxidation prosses that measured to determine the presence of oxidative stress due to damage by free radicals. The resent study was found that MDA levels in pregnant women were higher compared to non-pregnant women. Increased MDA levels are in line with the increase in gestational age, from the first, second, and third trimesters. Study was also found that there was a significant increase in MDA levels accompanied by a decrease in non-enzymatic antioxidants, such as Vitamin-E, Vitamin-C, and Vitamin-A in pregnant women with hypertension. Moreover this study assums that was "Giving Antioxidant to decrease Malondialdehyde (MDA) in the third trimester of pregnancy". futher more the aims of this study was analyzed effect of natural antioxidants treatment after exercise to MDA level in the third trimester of pregnancy.

This study was a experimental study. This study was located in the several clinics midwife practice, Community Health Centers (Puskesmas) in Palembang and Bio Sains Riset Laboratory Palembang from August to October 2016.

the study was approved informed consent from all participans as well as ethical clearance approval number is 007/UP-ETIK/I/2016. This study was obtained 24 prenatal pregnant women that separated to 3 groups, which were 8 prenatal pregnant women without pregnancy workout exercise as a control group, 8 prenatal pregnant women with pregnancy workout exercise as a positive control group and 8 prenatal pregnant women with consume natural antioxidants after pregnancy workout exercise as a treatment group.

The antioxidant compounds given in this study were natural vitamin C from 100 ml guava juice without additionaly sugar, 5 cc syringe, cotton, alcohol, syringe, hanscoon, EDTA tube, serum, TCA 5%, Na thiobarbiturat 0,65%, MDA standart, and aquades.

Spectrophotometric assay used to measure the MDA levels in the Bio Sains Riset Laboratory, Palembang and followed the essay of protocol kit from manufacture.

Comparison between groups used by one way ANOVA test, that was considered significant if p < 0.05. All statistical analyses were done by SPPS for Windows version 16.0.

Study Type

Interventional

Enrollment (Actual)

24

Phase

  • Not Applicable

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

20 years to 30 years (Adult)

Accepts Healthy Volunteers

No

Genders Eligible for Study

Female

Description

Inclusion Criteria:

  • Approve as a subject of review and sign in an informed consent
  • Approve to take 3 ml of whole blood.
  • Ages 20-30 years
  • Hemoglobin levels higher than 11 gr%
  • Third trimester of pregnancy
  • Healthy condition during reseach

Exclusion Criteria:

  • Anemia
  • Pathologies condition during pregnant
  • Allergic
  • Disability

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Treatment
  • Allocation: Randomized
  • Interventional Model: Parallel Assignment
  • Masking: Double

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
No Intervention: control group
pregnant women without pregnancy workout and consume guava juice
Experimental: positive control group
prenatal pregnant women with pregnancy workout but not consume guava juice
Other: pregnancy workout

Procedure of pregnancy workout has been legalized by the Ministry of Health of Indonesia act 2014 No.97.

100 ml fresh guava juice without sugar

Other Names:
  • fresh Guava juice
Experimental: treatment group
prenatal pregnant women with pregnancy workout and consume guava juice
Other: pregnancy workout

Procedure of pregnancy workout has been legalized by the Ministry of Health of Indonesia act 2014 No.97.

100 ml fresh guava juice without sugar

Other Names:
  • fresh Guava juice

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
MDA level
Time Frame: 7 days.
Malondialdehyde (MDA) levels is stress oxidative marker. MDA level messured with spectrophotometric assay. at Bio Sains Riset Laboratory, Palembang. Firstly, Mix 0.5ml blank + 0.5 ml standard and 0.5 ml serum using vortex mixer. Centrifuge for 10 minutes, with a speed of 2000 rpm, and Pipette 1 ml each filtrate and after that put it in boiling water bath for 30 minutes, then chill and read the absorbance with a spectrophotometer with a wavelength of 550 nm.
7 days.

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Indonesia University

Collaborators

Indonesia-MoH

Investigators

  • Principal Investigator: Ocktariyana Ocktariyana, Ministry of health, Health Polytechnic, Palembang

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

February 3, 2016

Primary Completion (Actual)

August 26, 2016

Study Completion (Actual)

October 17, 2016

Study Registration Dates

First Submitted

January 9, 2020

First Submitted That Met QC Criteria

January 9, 2020

First Posted (Actual)

January 13, 2020

Study Record Updates

Last Update Posted (Actual)

January 18, 2020

Last Update Submitted That Met QC Criteria

January 14, 2020

Last Verified

January 1, 2020

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • qwery123

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

No

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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