Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men (PECS)

June 9, 2023 updated by: Cameron Mitchell, University of British Columbia

The Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men: a Randomized Controlled Trial

The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Detailed Description

This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.

Study Type

Interventional

Enrollment (Actual)

17

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Contact

Study Contact Backup

Study Locations

  • Canada
    • British Columbia
      • Vancouver, British Columbia, Canada, V6T 1Z3
        • Chan Gunn Pavilion

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

18 years to 35 years (Adult)

Accepts Healthy Volunteers

Yes

Description

Inclusion Criteria:

  • Can fluently read and write in English
  • Male
  • 18 - 35 years

Exclusion Criteria:

  • Diabetes, cardiovascular disease, or current pharmaceutical therapy
  • Medical conditions impacting ability to undertake strenuous physical activity
  • Lower body resistance training in the previous 6 months
  • BMI > 30
  • Currently smoking with cigarettes
  • Allergy to any study supplements
  • Currently taking any protein, amino acid, or collagen supplement
  • Participate in greater than 2 hours of weekly vigorous exercise

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Basic Science
  • Allocation: Randomized
  • Interventional Model: Parallel Assignment
  • Masking: Triple

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Active Comparator: Essential Amino Acid
Participants will consume 1 dose of: 15 g crystalline essential amino acid supplement (Pure Encapsulation Essential Aminos 180) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water
Dietary supplement: Essential Amino Acid
Immediately following the exercise intervention participants will consume their assigned essential amino acid supplement
Active Comparator: Collagen Peptide
Participants will consume 1 dose of: 15g collagen peptide (Gelita TENDOFORTE) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water
Dietary supplement: Collagen Peptide
Immediately following the exercise intervention participants will consume their assigned collagen peptide supplement
Placebo Comparator: Maltodextrin Placebo
Participants will consume 1 dose of:15 g maltodextrin (Canadian Protein Maltodextrin) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water
Dietary supplement: Maltodextrin Placebo
Immediately following the exercise intervention participants will consume their assigned maltodextrin supplement

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
Time Frame: 0 - 4 hours post-exercise
Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.
0 - 4 hours post-exercise
Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
Time Frame: 0 - 2 hours post-exercise
Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.
0 - 2 hours post-exercise
Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
Time Frame: 2 - 4 hours post-exercise
Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.
2 - 4 hours post-exercise
Fractional synthetic rate (change over time) Muscle Collagen Synthesis
Time Frame: 0 - 4 hours post-exercise
Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.
0 - 4 hours post-exercise
Fractional synthetic rate (change over time) Muscle Collagen Synthesis
Time Frame: 0 - 2 hours post-exercise
Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.
0 - 2 hours post-exercise
Fractional synthetic rate (change over time) Muscle Collagen Synthesis
Time Frame: 2 - 4 hours post-exercise
Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.
2 - 4 hours post-exercise
Pro-Collagen Type I N-Propeptide (PINP)
Time Frame: 1 hour prior to exercise
Osteoblastic marker of bone collagen synthesis
1 hour prior to exercise
Pro-Collagen Type I N-Propeptide (PINP)
Time Frame: 4 hours post exercise
Osteoblastic marker of bone collagen synthesis
4 hours post exercise

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Plasma Amino Acids Response to Supplementation
Time Frame: 0 - 4 hours post-exercise
Amino acid plasma concentrations
0 - 4 hours post-exercise
Muscle mechanistic target of rapamycin Pathway Activation
Time Frame: 0 - 4 hours post-exercise
Measuring the fold change in enzymes associated with the anabolic signalling of the mTOR pathway (e.g. phosphoinositide 3-kinases (PI3k), protein kinase B (Akt))
0 - 4 hours post-exercise
PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV)
Time Frame: 0 - 4 hours post-exercise
Measuring the fold change when the collagen genes of interest become detectable in the PCR analysis
0 - 4 hours post-exercise
Pro-Collagen Type III N-Propeptide (PIIINP)
Time Frame: 1 hour prior to exercise
Marker of collagen synthesis
1 hour prior to exercise
Pro-Collagen Type III N-Propeptide (PIIINP)
Time Frame: 4 hours post exercise
Marker of collagen synthesis
4 hours post exercise

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

University of British Columbia

Investigators

  • Principal Investigator: Cameron Mitchell, PhD, University of British Columbia

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

January 25, 2021

Primary Completion (Actual)

February 13, 2022

Study Completion (Actual)

February 13, 2022

Study Registration Dates

First Submitted

September 28, 2020

First Submitted That Met QC Criteria

October 15, 2020

First Posted (Actual)

October 22, 2020

Study Record Updates

Last Update Posted (Actual)

June 13, 2023

Last Update Submitted That Met QC Criteria

June 9, 2023

Last Verified

June 1, 2023

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • H20-00943

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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