- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT06331481
The Effect of Over Weight on Oocytes Quality in IVF Patients (IVF)
Obesity is an increasing worldwide problem and a risk factor for many diseases. The effect of obesity on female fertility has been extensively tested. Obese women have increased time to pregnancy and remain subfertile even in the absence of ovulatory dysfunction. They present with higher incidence of infertility compared to normal weight women.
This study is prospective observational cohort study performed between February 2022 until June 2022 in a single university-affiliated hospital.
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
The effect of obesity on female fertility has been extensively tested. Obese women have increased time to pregnancy and remain subfertile even in the absence of ovulatory dysfunction. They present with higher incidence of infertility compared to normal weight women. Furthermore, obesity affects the outcome of fertility treatments, resulting in decreased ovarian response to stimulation, lower quantity and quality of oocytes, and adverse effects on the quality of embryos including lower blastocyst formation and on the chances of conception.
Subfertility and infertility research are aiming to find different markers that may shed light about oocyte quality. It is known that follicular fluid is the microenvironment of the oocyte. Both cumulus cells (CCs) and follicular fluid (FF) are critical determinants for oocyte and reflect its quality.
The oocyte in the ovary develops with the support of cumulus and granulosa cells, which are in close contact with her by gap junction. The oocytes are known to be unable to synthesize their own cholesterol and have very poor glucose metabolism to produce energy. The oocytes are supported for this purpose by cumulus and granulosa cells, and they basically reflect the quality of the oocytes. Evaluating different markers in the follicular fluid and cumulus cells including mitochondrial-DNA and protein that are known to be biomarkers of quality may help us to understand the impact of obesity on oocyte quality without harming the valuable resource.
In this study, the investigators aimed to evaluate the impact of obesity on oocyte quality using 2 main factors reflecting oocyte quality mtDNA levels in CCs and levels of proteins BMP-15 and HSPG2 in FFs.
Women younger than age 41 years, undergoing IVF/IVF-ICSI cycles, participated in the study. Patients were allocated into two BMI groups (<25 and >30) based on WHO criteria to evaluate distinctly different groups. The attending physician determined each patient's care plan without any relation to study recruitment. At the time of ultrasound-guided transvaginal ovum pick-up (OPU), only patients with four or more follicles were recruited. Demographic information was recorded from the electronic medical records, including demographics and obstetrical history (age, parity, BMI, number of previous pregnancies and abortions, prior IVF/ICSI cycles, and biochemical and basal hormonal profile), cycle characteristics (the stimulation protocol, endometrial thickness, and E2, LH and progesterone levels on hCG administration day), cycle outcomes (fertilization rate, number of oocytes, transferred and frozen embryos) and treatment outcomes (chemical and clinical pregnancy rate). Women who had undergone fertility preservation or had comorbidities, such as endocrine disorders, cancer, or other chronic diseases, were excluded.
Study Type
Enrollment (Actual)
Contacts and Locations
Study Locations
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-
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Hadera, Israel, 38100
- Hillel Yaffe Medical Center - IVF Unit
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
- Adult
Accepts Healthy Volunteers
Sampling Method
Study Population
Description
Inclusion Criteria:
- All women divided by BMI below 25 and above 25 who underwent IVF-ICSI treatment.
- Consent to participate in the study.
Exclusion Criteria:
- Patients older than 40 years
- Fertility preservation
- Unbalanced endocrine disorders
- Less than 4 follicles at the time of oocyte pick-up
Study Plan
How is the study designed?
Design Details
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Impact of obesity on oocyte's mtDNA expression in Cumulus Cells
Time Frame: 6 months
|
How obesity impacts oocyte quality through analysis of mtDNA expression in Cumulus Cells
|
6 months
|
Impact of obesity on oocyte quality through levels of protein BMP-15
Time Frame: 6 months
|
How obesity impacts oocyte quality through analysis of levels of protein BMP-15 in Follicular fluid.
|
6 months
|
Impact of obesity on oocyte quality through levels of protein HSPG2
Time Frame: 6 months
|
How obesity impacts oocyte quality through analysis of levels of protein HSPG2 in Follicular fluid.
|
6 months
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Impact of obesity on fertilization and pregnancy rates through analysis of mtDNA expression
Time Frame: 18 months
|
How obesity impacts fertilization and pregnancy rates through analysis of mtDNA expression in Cumulus Cells
|
18 months
|
Impact of obesity on fertilization and pregnancy rates through levels of protein BMP-15
Time Frame: 18 months
|
How obesity impacts fertilization and pregnancy rates through analysis of levels of protein BMP-15 in Follicular fluid.
|
18 months
|
Impact of obesity on fertilization and pregnancy rates through levels of protein HSPG2
Time Frame: 18 months
|
How obesity impacts fertilization and pregnancy rates through analysis of levels of protein HSPG2 in Follicular fluid.
|
18 months
|
Collaborators and Investigators
Sponsor
Investigators
- Principal Investigator: Einat Shalom-Paz, p, Hillel Yaffe Medical Center
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Estimated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- 0005-22-HYMC
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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