Immunological Characterization of Blood of Normal Individuals
研究概览
详细说明
Blood will be obtained via peripheral venipuncture. The blood will be divided into components (e.g. plasma, serum, microvesicles, and cells) then analyzed. Flow cytometry will be used to determine cell and microvesicle subsets. RNA will be extracted from the various components of blood and will be subjected to gene and microRNA profiling to determine gene expression. DNA will be isolated from the cells to be used in studies investigating epigenetic regulation of genes identified in the screens. Protein will also be isolated from the multiple components to examine the impact of genetic regulation by epigenetic mechanisms or microRNA on protein expression. Peripheral blood mononuclear cells will be placed in tissue culture for study.
Saliva and urine will be obtained the same day as blood for testing.
研究类型
注册 (实际的)
联系人和位置
学习地点
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Ohio
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Columbus、Ohio、美国、43210
- The Ohio State University
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
描述
Inclusion Criteria:
- Healthy with no chronic or acute illnesses, or taking medication which in the opinion of the PI would impact the analyses being conducted.
- At least 18 years or older.
- Are capable of reading, understanding and providing written informed consent.
Exclusion Criteria:
- Non-English speaking.
- Individuals under 18 years old.
- Woman who believe they are or may be pregnant. (Based on self reporting. Pregnancy testing will not be conducted for the purposes of this study)
- Individuals experiencing acute or chronic illnesses which in the opinion of the PI would impact the analyses being conducted.
- Currently taking prescription or over the counter medication which in the opinion of the PI would impact the analyses being conducted.
- Individual has donated blood more than twice in the last week or the amount to be drawn will exceed the maximum allowance in the last 8 weeks.
学习计划
研究是如何设计的?
设计细节
队列和干预
团体/队列 |
干预/治疗 |
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Healthy Volunteer
Healthy Volunteer without lung disease
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这项研究没有干预
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研究衡量的是什么?
主要结果指标
结果测量 |
措施说明 |
大体时间 |
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Blood content differences between the lung disease and healthy control populations
大体时间:Day 1
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Test for differences in the blood of individuals with lung disease and normal healthy controls.
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Day 1
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
---|---|---|
Genetic expression of relevant genes between the lung disease and healthy control populations
大体时间:Day 1
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Test for genetic differences in the blood of individuals with lung disease and normal healthy controls.
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Day 1
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Saliva and urine analysis results compared to blood content results between the lung disease and healthy control populations.
大体时间:Day 1
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Analyze saliva and urine along with the blood sample in order to compare the information in the blood with the saliva and urine.
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Day 1
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Identify diagnostic and prognostic indicators for lung disease
大体时间:December 2020
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Develop and test possible diagnostic and prognostic indicators for various lung diseases.
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December 2020
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Develop possible treatment for lung disease
大体时间:December 2020
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Develop and test possible reprogramming of blood cells to promote vessel formation as a way to prevent tumor metastases and modify the progression of specific lung diseases.
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December 2020
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合作者和调查者
调查人员
- 首席研究员:Clay Marsh, M.D.、Ohio State University
研究记录日期
研究主要日期
学习开始 (实际的)
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (估计)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
与本研究相关的术语
其他研究编号
- 2011H0007
此信息直接从 clinicaltrials.gov 网站检索,没有任何更改。如果您有任何更改、删除或更新研究详细信息的请求,请联系 register@clinicaltrials.gov. clinicaltrials.gov 上实施更改,我们的网站上也会自动更新.
无干预的临床试验
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University of Illinois at ChicagoShirley Ryan AbilityLab; Oakland University; Access Living主动,不招人
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University of Wisconsin, MadisonNational Cancer Institute (NCI); Northwestern University主动,不招人
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Otsuka Pharmaceutical Factory, Inc.Celerion完全的