Prevalence of Bacteria Following Endodontic Treatment

Prevalence and Phenotype of Oral Enterococcus Faecalis

Sampling of accessed root canals with endodontic paper points during treatment and immediately pre-obturation of endodontic root canals was carried out.

Study Overview

• Status: Unknown
• Conditions: Root Canal Infection
• Intervention / Treatment: Diagnostic test: Detection of residual vital bacteria in the root canal space

Detailed Description

Following ethical approval (REC ref: 05/Q0705/051), informed consent was obtained from patients undergoing root canal treatment.The teeth included in the study had been referred to a specialist centre for the treatment of endodontic infection. They all responded negatively to vitality tests and presented pre-operative apical radiolucencies. The source population were referred by their general dental practitioner into specialist endodontic clinics of Guy's Hospital, King's College London Dental Institute.Treatments on the teeth were carried out by a total of 19 postgraduate endodontic students.

• Study Type: Observational
• Enrollment (Anticipated): 200

Contacts and Locations

• Study Contact: Name: Federico Foschi, Dr; Phone Number: +44 (0)020 7188 7188; Email: federico.foschi@kcl.ac.uk
• Study Contact Backup: Name: Dylan Herzog, Mr; Phone Number: +44 (0)207 188 1789; Email: dylan.b.herzog@kcl.ac.uk

Study Locations

• United Kingdom
  • London, United Kingdom, SE1 9RT
    • Recruiting
    • King's College London, Dental Institute, Floor 22, Endodontics
    • Contact: Francesco Mannocci, Prof; Phone Number: +44 (0)20 7188 7188; Email: francesco.mannocci@kcl.ac.uk

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 65 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

The source population are adults aged 18-65, referred by their general dental practitioner into specialist endodontic clinics of Guy's Hospital, King's College London Dental Institute. Both single- and multi-rooted non-vital teeth are included in the study

Description

Inclusion Criteria:

• Patients with single or multi-rooted teeth undergoing root canal treatment.

Exclusion Criteria:

• For certain aspects of the study secondary treatments and vital teeth are excluded.

Study Plan

How is the study designed?

Design Details

• Observational Models: Cohort
• Time Perspectives: Cross-Sectional

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Primary treatments; Patients undergoing primary root canal treatments. Only teeth which have not previously been accessed are included in this cohort.The source population are adults aged 18-65, referred by their general dental practitioner into specialist endodontic clinics of Guy's Hospital	Diagnostic test: Detection of residual vital bacteria in the root canal space; Detection of residual vital bacteria in the root canal space. No intervention, as it is an observational study. Vital bacteria are looked for in the samples taken from the root canal space.; Other Names: Detection of root canal infection
Re-treatments; Patients undergoing re-treatments. Only teeth which had a previous root canal treatment which failed are included in this cohort.The source population are adults aged 18-65, referred by their general dental practitioner into specialist endodontic clinics of Guy's Hospital	Diagnostic test: Detection of residual vital bacteria in the root canal space; Detection of residual vital bacteria in the root canal space. No intervention, as it is an observational study. Vital bacteria are looked for in the samples taken from the root canal space.; Other Names: Detection of root canal infection

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Detection of residual bacteria during and post root canal treatment	Using a vital fluorescent stain bacteria sampled from the root canal space were detected during and post root canal treatment.	June 2016

Collaborators and Investigators

• Sponsor: King's College London
• Collaborators: Guy's and St Thomas' NHS Foundation Trust
• Investigators: Principal Investigator: Federico Foschi, Dr, King's College London

Publications and helpful links

General Publications

• Herzog DB, Hosny NA, Niazi SA, Koller G, Cook RJ, Foschi F, Watson TF, Mannocci F, Festy F. Rapid Bacterial Detection during Endodontic Treatment. J Dent Res. 2017 Jun;96(6):626-632. doi: 10.1177/0022034517691723. Epub 2017 Feb 1.

Study record dates

Study Major Dates

• Study Start (Actual): January 30, 2013
• Primary Completion (Anticipated): May 30, 2017
• Study Completion (Anticipated): October 30, 2018

Study Registration Dates

• First Submitted: February 14, 2017
• First Submitted That Met QC Criteria: February 14, 2017
• First Posted (Actual): February 16, 2017

Study Record Updates

• Last Update Posted (Actual): February 17, 2017
• Last Update Submitted That Met QC Criteria: February 16, 2017
• Last Verified: February 1, 2017

More Information

Terms related to this study

• Keywords: Bacteria; Endodontics; Root canal treatment
• Other Study ID Numbers: 05/Q0705/051

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: Undecided
• IPD Plan Description: IPD is not planned to be made available to other researchers as of yet

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No