- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT05659940
Correlation Between a Novel Subset of Neutrophil and Corneal Neovascularization.
Study Overview
Status
Conditions
Detailed Description
Cornea is an avascular transparent tissue providing refractive power for suitable optical performance. But under the condition of injury or infection, the stable microenvironment of ocular surface has been destroyed. Vessels sprout from the limbus to form corneal neovascularization, becoming an important pathological feature and leading cause of blindness. However, the underlying mechanism particular the inflammation-mediated mechanism remains unclear.
With the advancement of single-cell RNA sequencing (scRNA-seq), the investigation of immune cell transcriptomes has obtained great process . Using this advanced method, we identified different immune cell types in the ocular surface microenvironment in corneal neovascularization mouse model. Particularly, we found a specific subset of neutrophils played important role during the corneal angiogenesis. Using the loss-of-function and gain-of-function experiment, we clarified that this novel subset of neutrophils is the major factor that promoting corneal angiogenesis in mouse model. To further explore the relationship between this subset of neutrophils and angiogenesis in human, we plan to detect whether the neutrophil subset is increased in the circulation of chemical burned corneal neovascularization patients.
The main purpose of this study is to assess whether the novel subset of neutrophils has correlation with chemical burned corneal neovascularization. Approximately 33 patients and 33 age matched healthy controls will be recruited. The clinical characteristics, 4ml peripheral blood and the images of slit lamp photography will be obtained.
Study Type
Enrollment (Anticipated)
Contacts and Locations
Study Contact
- Name: Ziqi Yang
- Phone Number: 8618826227484
- Email: yangzq6@mail2.sysu.edu.cn
Study Locations
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Guangdong
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Guangzhou, Guangdong, China, 510000
- Recruiting
- Zhongshan Ophthalmic Center
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Contact:
- Ziqi Yang, PhD
- Phone Number: 8618826227484
- Email: yangzq6@mail2.sysu.edu.cn
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Principal Investigator:
- Xialin Liu, Prof.
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Sampling Method
Study Population
Description
Inclusion Criteria:
Diagnosed ocular chemical injury with or without corneal neovascularization.
Exclusion Criteria:
- Those who are diagnosed ocular diseases except for chemical ocular injury, such as uveitis, retinopathy.
- Those who are diagnosed allergic disease, autoimmune diseases or hematological diseases, infectious disease, such as eczema, systemic lupus erythematosus (SLE), agranulocytosis and viral hepatitis.
- Those who took immunosuppressive treatments in the past 3 months.
- Pregnant or nursing women.
- Those who have serious heart, lung, liver or kidney diseases.
- Those who have serious systemic diseases.
- Those who performed ocular surgery (including cataract surgery) in recent three months.
- Those who have been enrolled in other interventional clinical studies at the same time.
- Those who are unable to complete the study according to the investigators' requirements.
Study Plan
How is the study designed?
Design Details
- Observational Models: Case-Control
- Time Perspectives: Cross-Sectional
Cohorts and Interventions
Group / Cohort |
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Healthy people
Healthy participants whose age and gender are consistent with CNV patients
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CNV patients
Patients who are diagnosed ocular chemical injury coming to Zhongshan Eye Center, Sun Yat-sen University for medical care
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What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Changes of angiogenic neutrophils ratio
Time Frame: Inclusion (Day1) and 1 week
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Peripheral blood is collected in EDTA coated tubes and the red blood cells are lysed.
The cells are firstly blocked and then stained the neutrophil subset markers.
Flow cytometric analysis is performed at BD LSRFortessa™ Cell Analyzer and cells are analyzed by FlowJo v10 software.
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Inclusion (Day1) and 1 week
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Corneal Neovascularization Score
Time Frame: Inclusion (Day1) and 1 week
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The neovascularization is scored on a scale of 0-3 as previously reported.
0 = no vessels; 1 = neovessels at the corneal limbus; 2 = neovessels spanning approaching the corneal center; 3 = neovessels spanning the central cornea.
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Inclusion (Day1) and 1 week
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Collaborators and Investigators
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Anticipated)
Study Completion (Anticipated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Estimate)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- 2022KYPJ226
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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