Molecular Signature of Inactivity Induced Exercise Responsiveness

Fitness is one of the best predictors for heart and brain disease. To increase ones fitness, the American Heart Association (AHA) says to exercise at least 150 minutes per week or 75 minutes per week if really hard. These exercise guides are pretty effective, however not everyone will get the same results. What individuals do outside of the exercise bout can influence the effectiveness of exercise. One of these factors is our time sitting, which has caused the phrase "sitting is the new smoking". Other studies have said that the metabolic benefits of exercise are decreased when you exercise after a few days of low activity (less than 5,000 steps per day). This is important in that exercise may not be able to fully offset these times of inactivity. However, these studies were only looking at different fats in the blood. As exercise increases fat burn up to 10 times in the muscle, more research is needed to understand how inactivity affects the muscle during exercise and after exercise. This study will help answer two questions: 1) How does a day of sitting a lot affect the muscle's ability to respond to exercise? and 2) How does a day of sitting a lot affect carbohydrate and fat burn during and after a bout of exercise? The investigators will answer these questions by having people complete one day of inactivity (less than 5,000 steps) or normal activity (more than 8,500 steps). Subjects will then come in the next day to bike somewhat hard for 1 hour. The investigators will take blood samples before, during, and after exercise to measure energy sources. The investigators will also collect pieces of skeletal muscle before and after exercise to see how the muscle responded to exercise. This study is significant for the publication of exercise guidelines to minimize risk of heart and metabolic diseases.

Study Overview

• Status: Recruiting
• Conditions: Metabolic Diseases; Aerobic Exercise
• Intervention / Treatment: Other: Inactivity

• Study Type: Interventional
• Enrollment (Estimated): 10
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Kevin J Gries, PhD; Phone Number: 262-243-4293; Email: kevin.gries@cuw.edu

Study Locations

• United States
  • Wisconsin
    • Mequon, Wisconsin, United States, 53092
      • Recruiting
      • Kevin J Gries
      • Contact: Kevin J Gries, PhD; Phone Number: 262-243-4293; Email: kevin.gries@cuw.edu

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• free from acute or chronic illness (cardiac, pulmonary, liver, or kidney abnormalities, cancer, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other known metabolic disorders)
• free from orthopedic limitations (including any artificial joints) no known lidocaine allergy
• do not currently smoke or participate in other forms of tobacco use.
• not currently in a structured exercise program

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Inactive; Subjects will be asked to be sedentary prior to an acute exercise bout (<5,000 steps as measured by a physical activity monitor).	Other: Inactivity; Low physical activity level prior to the exercise intervention.; Other Names: Sedentary
Placebo Comparator: Normal activity; Subjects will be asked to have normal activity prior to an acute exercise bout (>8,500 steps as measured by a physical activity monitor).	Other: Inactivity; Low physical activity level prior to the exercise intervention.; Other Names: Sedentary

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Circulating lipid and carbohydrate metabolism	Investigate the role of sex and inactivity on lipid and carbohydrate metabolism following a bout of aerobic exercise.	Baseline; 20 and 40, and 60 minutes into exercise; and every 30 minutes after exercise up to 4 hours
Basal and post-exercise gene expression with inactivity	Study the role of inactivity on basal and exercise-induced gene expression in skeletal muscle.	Baseline; 4 hours after exercise

Collaborators and Investigators

• Sponsor: Concordia University Wisconsin

Study record dates

Study Major Dates

• Study Start (Actual): January 1, 2024
• Primary Completion (Estimated): December 31, 2025
• Study Completion (Estimated): December 31, 2025

Study Registration Dates

• First Submitted: February 29, 2024
• First Submitted That Met QC Criteria: March 6, 2024
• First Posted (Actual): March 8, 2024

Study Record Updates

• Last Update Posted (Actual): March 8, 2024
• Last Update Submitted That Met QC Criteria: March 6, 2024
• Last Verified: March 1, 2024

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Metabolic Diseases
• Other Study ID Numbers: FY24-62

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: YES

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No